puriFlash® 5.250 High-Pressure Preparative Purification System
| Brand | Advion Interchim Scientific |
|---|---|
| Origin | France |
| Model | puriFlash® 5.250 |
| Maximum Flow Rate | 250 mL/min |
| Maximum Pressure | 250 bar |
| Import Category | Imported Instrument |
| Distributor Type | Authorized Distributor |
| Pricing | Available Upon Request |
Overview
The puriFlash® 5.250 is a high-performance, modular preparative chromatography system engineered by Advion Interchim Scientific for robust, scalable purification of complex organic, pharmaceutical, and natural product mixtures. Built on a dual-pump high-pressure liquid chromatography (HPLC) architecture, it operates under precise gradient control and delivers stable flow rates up to 250 mL/min at pressures up to 250 bar—enabling efficient separation across a broad range of stationary phases, including silica, C18, amino, cyano, and specialized chiral or polymer-based media. Its design adheres to core principles of flash chromatography scalability and HPLC-grade resolution, bridging the gap between analytical method development and multi-gram preparative isolation. The system is fully compatible with both UV/Vis and mass-directed fraction collection workflows, supporting seamless transition from scouting runs to process-scale purification in R&D and early-stage API manufacturing environments.
Key Features
- High-pressure dual-gradient pump system delivering pulse-free flow from 0.1 to 250 mL/min with <±0.5% flow accuracy and <0.1% RSD over extended operation.
- Integrated 250 bar pressure rating with real-time pressure monitoring, automatic overpressure shutdown, and solvent compressibility compensation algorithms.
- Modular architecture supporting up to four independent UV/Vis wavelength channels (200–400 nm), optional ELSD or MS-triggered fraction collection, and interchangeable column cartridges (4–100 mm i.d., up to 300 mm length).
- Intuitive touchscreen interface with preloaded method templates, customizable gradient profiles, and embedded SOP-driven workflow guidance compliant with GLP documentation requirements.
- Corrosion-resistant fluidic path constructed from PEEK, stainless steel (316L), and sapphire components—validated for use with aggressive solvents including DCM, THF, DMF, and TFA-containing mobile phases.
Sample Compatibility & Compliance
The puriFlash® 5.250 accommodates crude reaction mixtures, natural extract fractions, peptide libraries, and small-molecule APIs without prior filtration or extensive sample cleanup—provided dissolved solids remain below 5% w/v. It supports standard flash columns (glass, polypropylene, stainless steel) and certified prepacked cartridges meeting ASTM D7527 and ISO 17025 traceability criteria. All hardware and firmware comply with IEC 61010-1 safety standards and CE marking requirements. Data acquisition and audit trails meet FDA 21 CFR Part 11 readiness when paired with Interchim’s puriFlash® Control Software v5.x, including electronic signatures, user access levels, and immutable method versioning.
Software & Data Management
puriFlash® Control Software provides full instrument control, real-time chromatogram visualization, peak integration, and fraction decision logic—including UV threshold, slope, and mass-triggered collection modes. Raw data are stored in vendor-neutral .csv and .cdf formats; method files include complete mobile phase composition history, pump calibration logs, and column usage tracking. The software supports LIMS integration via OPC UA and exports metadata compliant with ISA-88 batch record structures. Audit trails record all user actions, parameter changes, and system alerts with timestamped, digitally signed entries—fully retrievable for internal audits or regulatory inspections.
Applications
- Rapid purification of synthetic intermediates and final compounds in medicinal chemistry campaigns (1–50 g scale).
- Isolation of bioactive constituents from plant extracts, fermentation broths, or marine-derived samples.
- Chiral separation of enantiomers using immobilized polysaccharide or Pirkle-type columns under high-flow conditions.
- Desalting and buffer exchange of peptides and oligonucleotides prior to lyophilization or characterization.
- Parallel purification of compound libraries using automated column switching and fraction pooling strategies.
FAQ
What column dimensions and chemistries are supported?
Standard compatibility includes columns from 4 mm to 100 mm i.d. and lengths up to 300 mm, with validated performance on silica, reversed-phase C18/C8, diol, NH₂, CN, and chiral stationary phases (e.g., Chiralpak AD-H, IC).
Can the system be integrated with a mass spectrometer for real-time fraction triggering?
Yes—via analog TTL output or Ethernet-based API communication with Advion expression® CMS or third-party LC-MS platforms supporting Mass Directed Collection protocols.
Is method transfer from analytical HPLC to puriFlash® 5.250 straightforward?
Scalable method translation is supported through built-in retention time normalization, linear velocity matching, and automatic flow/gradient scaling tools within the software.
What maintenance intervals are recommended for routine operation?
Pump seal replacement every 6 months under continuous use; UV lamp recalibration quarterly; system backflush and leak testing performed before each campaign per SOP-PUF-003.
Does the system support GMP-compliant operation out of the box?
While the base configuration meets GLP requirements, full 21 CFR Part 11 compliance requires activation of software audit trail modules, role-based access control setup, and documented IQ/OQ protocols provided by Interchim’s Validation Support Package.
