ROCKER ROCKER300C-1L Vacuum Liquid Aspiration System
| Brand | ROCKER |
|---|---|
| Origin | Taiwan |
| Model | ROCKER300C-1L |
| Ultimate Vacuum | 99 mbar |
| Maximum Flow Rate | 17 mL/sec |
| Dimensions | 285 × 135 × 204 mm |
| Bottle Capacity | 1 L (GL45) |
| Pump Type | Oil-Free Diaphragm |
| Wetted Materials | PTFE-Coated Housing, Borosilicate Glass Bottle, PTFE Hydrophobic Filter, Platinum-Cured Silicone Tubing |
| Noise Level | <50 dB(A) |
| Power Supply | 220 V / 50 Hz |
Overview
The ROCKER ROCKER300C-1L Vacuum Liquid Aspiration System is an oil-free, diaphragm-based vacuum pump engineered for precise, safe, and chemically resistant liquid handling in academic, clinical, and industrial laboratories. It operates on the principle of positive displacement via a corrosion-resistant PTFE-coated diaphragm mechanism—eliminating the need for lubricating oil, minimizing maintenance, and ensuring consistent vacuum delivery across variable load conditions. Designed specifically for aspiration of supernatants, cell culture media, spent reagents, acidic or basic aqueous solutions, and volatile organic solvents, the system integrates intrinsic chemical compatibility with functional modularity. Its ultimate vacuum of 99 mbar supports efficient removal of low-viscosity liquids while maintaining operational stability under intermittent or continuous duty cycles. The system’s architecture prioritizes user safety and equipment longevity through integrated hydrophobic barrier protection, adjustable vacuum regulation, and full wet-path material compliance with ISO 8573-1 (Class 3 compressed air purity) and laboratory-grade chemical resistance standards.
Key Features
- Oil-free diaphragm pump design ensures zero oil contamination risk, eliminates oil replenishment, and reduces long-term ownership cost.
- PTFE-coated pump housing and internal components provide broad-spectrum resistance to halogenated solvents (e.g., chloroform, DCM), strong acids (HCl, HNO₃), caustics (NaOH), and oxidizing agents—validated per ASTM D543 and ISO 175.
- Integrated hydrophobic PTFE filter (0.2 µm pore rating) prevents liquid ingress into the pump chamber during accidental siphon reversal or overfilling; filter is replaceable and autoclavable (up to 121°C, 20 min).
- Adjustable vacuum regulator enables fine-tuned control of suction force (99–300 mbar range) and flow velocity—critical for delicate cell pellet recovery or viscous sample transfer.
- Modular GL45-threaded bottle interface accommodates standard borosilicate glass containers (e.g., Schott Duran, Kimax); users may interchange 500 mL, 1 L, or 2 L bottles without adapter modification.
- Low-noise operation (<50 dB[A] at 1 m distance) complies with ISO 7779 and supports use in shared lab environments, biosafety cabinets, and cleanroom-adjacent workflows.
Sample Compatibility & Compliance
The ROCKER300C-1L is validated for aspiration of aqueous buffers (PBS, Tris-HCl), ethanol/water mixtures, acetone, ethyl acetate, isopropanol, dilute HCl (≤1 M), NaOH (≤1 M), and cell culture supernatants containing serum proteins or nucleic acid fragments. It is not recommended for concentrated oxidizers (e.g., >30% H₂O₂), hydrofluoric acid, or molten salts. All fluid-contact materials meet USP Class VI biocompatibility requirements. The system conforms to IEC 61010-1:2010 (Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use) and CE marking directives. Optional footswitch integration supports hands-free operation under ISO/IEC 17025-accredited quality management systems.
Software & Data Management
As a standalone analog vacuum aspiration platform, the ROCKER300C-1L does not incorporate digital controllers or embedded software. Vacuum setpoint adjustment is performed manually via calibrated rotary valve; no firmware, data logging, or electronic audit trail is provided. This architecture aligns with laboratories operating under GLP or GMP frameworks where mechanical simplicity, traceable manual calibration, and absence of electronic validation burdens are operationally advantageous. For facilities requiring 21 CFR Part 11-compliant recordkeeping, external pressure transducers (e.g., Validyne DP15) may be connected via the auxiliary vacuum port for third-party data acquisition.
Applications
- Centrifuge tube and microplate supernatant removal in molecular biology and immunology workflows.
- Decanting spent media from adherent or suspension cell cultures prior to trypsinization or lysis.
- Vacuum-assisted filtration using GL45-mounted filter funnels (e.g., Whatman GD/X, Sartorius Minisart).
- Waste collection during electrophoresis buffer exchange or chromatography system priming.
- Controlled aspiration of diagnostic specimens (urine, CSF, pleural effusions) in clinical pathology labs.
- Reagent recovery and solvent recycling in synthetic chemistry fume hood setups.
FAQ
What is the maximum allowable temperature of liquids aspirated by the ROCKER300C-1L?
The system is rated for liquids up to 40°C ambient temperature; prolonged exposure to >50°C may degrade the platinum-cured silicone tubing and silicone O-rings.
Can the unit be used with non-GL45 bottles?
No—only GL45-threaded containers ensure proper sealing and pressure integrity; adapters for other thread types (e.g., GL32, GL25) are not supported or validated.
Is the PTFE hydrophobic filter sterilizable?
Yes—the filter cartridge can be autoclaved at 121°C for 20 minutes; however, repeated sterilization cycles may reduce hydrophobicity after ≥10 cycles.
Does the pump require periodic calibration?
No formal calibration is required; the vacuum regulator is factory-set and mechanically stable—users may verify performance using a certified digital vacuum gauge traceable to NIST standards.
What maintenance intervals are recommended?
Inspect tubing and filter monthly; replace PTFE filter every 6 months under continuous use or after 500 L total throughput; diaphragm service life exceeds 10,000 hours per ISO 13849-1 MTBF estimation.
