Ruhua RH-LHP-400L Artificial Climate Incubator

Overview

The Ruhua RH-LHP-400L Artificial Climate Incubator is a precision-engineered environmental simulation system designed for controlled plant physiology studies, seed germination trials, photoperiodic growth experiments, and long-term biological sample conditioning under reproducible temperature, humidity, and illumination regimes. Built upon a dual-wall insulated chamber with microprocessor-based PID control architecture, the unit maintains stable setpoints across its full operational envelope using independent refrigeration, heating, humidification, and multi-directional LED lighting subsystems. Unlike standard incubators limited to thermal regulation, this model integrates synchronized climatic parameter programming—enabling users to define up to 24 discrete time segments per day, each with unique combinations of temperature, relative humidity, and light intensity. Its triple-side illumination array ensures uniform photon flux distribution across the entire 400 L working volume, minimizing edge effects critical in morphological and photomorphogenic assays.

Key Features

• Programmable multi-segment climate profiles (1–24 segments/day) supporting diurnal cycling, vernalization protocols, and stress acclimation studies
• Triple-side high-efficiency LED lighting system delivering 0–10,000 lx with six-step intensity adjustment and independent on/off scheduling
• High-fidelity temperature control: ±0.5 °C accuracy and ±0.1 °C uniformity across the chamber interior, validated per ASTM E2874-13 guidelines for environmental chamber performance
• Humidity regulation from 40–95 %RH via ultrasonic humidifier and integrated condensation recovery; accuracy maintained within ±3–5 %RH under steady-state conditions
• Stainless-steel interior chamber with seamless welds and rounded corners for easy decontamination and compliance with GLP cleaning validation requirements
• Intuitive touchscreen interface with real-time graphing, data logging (internal SD card backup), and USB export capability for audit-ready records
• Robust mechanical design featuring double-glazed observation window, overtemperature/overhumidity safety cutoffs, and door-open alarm with automatic shutdown

Sample Compatibility & Compliance

The RH-LHP-400L accommodates standard plant growth containers—including Petri dishes, Magenta boxes, growth pouches, and hydroponic trays—across three adjustable stainless-steel shelves. Its internal dimensions (550 × 580 × 1350 mm) support vertical stacking of multi-tiered culture racks without compromising airflow or light penetration. The unit conforms to ISO 9001:2008 quality management standards throughout design, production, and calibration. While not intrinsically 21 CFR Part 11 compliant, its data logging architecture supports traceable electronic records when deployed with external validated software and user-defined access controls—making it suitable for preclinical botanical research environments operating under GLP or ISO/IEC 17025 frameworks.

Software & Data Management

The embedded controller firmware provides local data storage for up to 30 days of continuous 1-minute interval monitoring (temperature, humidity, light status). Logged parameters are timestamped and exportable in CSV format via USB interface for integration into LIMS or statistical analysis platforms (e.g., R, Python pandas, JMP). Optional Ethernet connectivity enables remote monitoring through secure HTTP(S) endpoints compatible with enterprise IT infrastructure. Audit trail functionality—recording operator ID, parameter changes, and alarm events—is configurable to meet institutional SOPs for instrument qualification (IQ/OQ/PQ) documentation.

Applications

• Controlled-environment plant phenotyping and ecophysiology studies requiring precise photoperiod and thermoperiod modulation
• Seed dormancy breaking, stratification, and germination rate optimization under standardized humidity and light spectra
• Cultivation of phototrophic microalgae and cyanobacteria under tunable PAR (Photosynthetically Active Radiation) conditions
• Pre-acclimation of tissue-cultured explants prior to ex vitro transfer
• Long-term stability testing of biologics, botanical extracts, and formulated agricultural adjuvants under accelerated aging protocols
• Supporting ISO 14644-1 classified cleanroom adjacent workflows where ambient environmental drift must be excluded

FAQ

Does the RH-LHP-400L support external sensor inputs for custom probe integration?

Yes—the rear panel includes analog input terminals (0–5 V DC) compatible with third-party temperature, CO₂, or PAR sensors, allowing expansion beyond factory-calibrated parameters.
Can humidity be maintained below 40% RH?

No—the minimum specified humidity range is 40%RH due to physical limitations of the ultrasonic humidification system and chamber sealing integrity.
Is the lighting spectrum customizable (e.g., red/blue ratio)?

No—the integrated LED array emits broad-spectrum white light (400–700 nm); spectral tuning requires external lamp systems.
What is the recommended calibration frequency for routine use?

Annual verification against NIST-traceable reference standards is advised; internal self-diagnostics run at startup and during idle cycles.
Are replacement trays and gaskets available as spare parts?

Yes—stainless-steel trays (P/N RH-T400-TRAY), door gaskets (P/N RH-GSKT-400), and humidifier cartridges (P/N RH-HUM-CAR) are stocked and ship globally.