Shen’an LDZX-30L Vertical Steam Autoclave

Overview

The Shen’an LDZX-30L Vertical Steam Autoclave is a CE-compliant, Class I pressure vessel engineered for reliable, repeatable moist-heat sterilization in academic, clinical, and industrial laboratory settings. It operates on the fundamental principle of saturated steam under controlled pressure and temperature—leveraging the thermodynamic properties of water vapor at elevated pressures to achieve microbial inactivation across bacterial spores, viruses, and prions. With a rated working pressure of 0.142 MPa and a maximum sterilization temperature of 126 °C, the unit conforms to internationally recognized sterilization cycles defined in ISO 17665-1 and EN 285 for steam sterilizers. Its vertical orientation optimizes floor space utilization while maintaining full accessibility to the 30 L chamber—ideal for routine processing of culture media, surgical instruments, glassware, pipette tips, and biohazard waste containers. The chamber’s AISI 304 stainless steel construction (≥2 mm wall thickness) ensures long-term corrosion resistance and compliance with FDA-recommended material standards for reusable sterilization equipment.

Key Features

• Microprocessor-controlled sterilization cycle with dynamic LCD interface displaying real-time temperature, pressure, elapsed time, and phase status (preheat, sterilize, exhaust, cooling)
• One-touch program selection for common load types: liquid media, solid instruments, wrapped goods, and pre-vacuum-compatible configurations
• Forced-air rapid cooling system with integrated axial fan reduces post-cycle cooldown time by up to 40%, minimizing operator wait time and improving throughput
• Programmable delayed start function supports unattended operation—ideal for overnight or off-shift sterilization scheduling
• Pre-melt mode enables precise temperature ramping (50–126 °C) and dwell control for agar-based media preparation without boil-over or thermal degradation
• Dual safety architecture: mechanical interlock prevents lid opening during pressurized operation; redundant overpressure protection via spring-loaded safety valve (0.142–0.165 MPa range) and electronic pressure cutoff
• Automatic cold air purge eliminates non-condensable gases prior to sterilization, ensuring uniform steam penetration and validated lethality (F₀) delivery
• Self-diagnostic firmware continuously monitors sensor integrity, heater performance, water level, and electrical grounding—logging fault codes for traceable maintenance
• Standard validation port supports third-party temperature mapping (e.g., with calibrated thermocouples or wireless data loggers) per ISO 13485 and EU Annex 1 requirements

Sample Compatibility & Compliance

The LDZX-30L accommodates a wide range of sterilizable materials—including aqueous solutions, dry solids, porous loads, and heat-stable plastics—within its Ø350 × 400 mm cylindrical chamber. Its design adheres to national and international regulatory frameworks governing sterilization equipment: GB/T 30690–2014 (China), ISO 17665-1:2017 (sterilization by moist heat), and EN 61010-1 (safety requirements for electrical lab equipment). While not certified to ASME BPVC Section VIII Div. 1, its manufacturing license issued under China’s Special Equipment Safety Law confirms compliance with mandatory pressure vessel design, fabrication, and inspection protocols. The unit meets baseline requirements for GLP and GMP environments where audit-ready documentation, calibration traceability, and process reproducibility are essential. Optional printer and USB data export support 21 CFR Part 11–aligned record retention when configured with time-stamped, tamper-evident logs.

Software & Data Management

The embedded control system implements deterministic real-time logic without reliance on external operating systems. All cycle parameters—including setpoints, actual profiles, alarms, and timestamps—are stored internally in non-volatile memory. When equipped with the optional USB module, users can export CSV-formatted sterilization records directly to a standard USB flash drive, enabling offline analysis in Excel or LIMS integration. The dot-matrix printer interface outputs hard-copy reports containing date/time stamp, chamber temperature curve summary, peak pressure, total exposure duration, and operator ID field (if enabled via password-protected login). Audit trails include automatic logging of parameter changes, door open/close events, and fault resets—supporting internal quality reviews and regulatory inspections under ISO 9001 and ISO/IEC 17025.

Applications

• Routine sterilization of microbiological growth media (e.g., nutrient broth, LB agar, Sabouraud dextrose agar)
• Decontamination of reusable labware: Petri dishes, test tubes, flasks, forceps, and scissors
• Preparation of sterile saline, buffer solutions, and reagent-grade water
• Processing of biohazardous waste in BSL-2 laboratories prior to autoclave validation or incineration
• Validation of sterilization protocols using biological indicators (e.g., Geobacillus stearothermophilus spore strips)
• Supporting ISO 11138-3 and ISO 11140-1 compliant sterility assurance level (SAL) verification

FAQ

What is the maximum allowable load volume for optimal sterilization efficacy?
For consistent steam penetration and F₀ delivery, the recommended maximum load is 70% of chamber volume (≤21 L), especially for wrapped or porous items. Overloading may impede air removal and cause cold spots.
Does the LDZX-30L support vacuum-assisted air removal?
No—it relies on gravity displacement for air purge. For vacuum-assisted cycles (e.g., wrapped instruments or lumened devices), a pre-vacuum autoclave model is required.
Is calibration documentation provided with the unit?
Yes: Each unit ships with a factory calibration certificate for the PT100 temperature sensor and Bourdon-tube pressure gauge, traceable to national metrology institutes.
Can the device be integrated into a centralized lab monitoring network?
Not natively—the LDZX-30L lacks Ethernet or RS-485 ports. However, USB data exports can be ingested into enterprise LIMS or SCADA systems via secondary gateways.
What maintenance intervals are recommended?
Daily: Check water level, clean chamber gasket and drain filter. Quarterly: Inspect safety valve function and verify pressure sensor drift (<±0.5 kPa). Annually: Full third-party validation including temperature mapping and Bowie-Dick testing (if applicable).