Sineo SPE100/200/400 Fully Automated Solid Phase Extraction System
| Brand | Sineo |
|---|---|
| Origin | Shandong, China |
| Manufacturer Type | Direct Manufacturer |
| Product Category | Domestic |
| Model | SPE100 / SPE200 / SPE400 |
| Automation Level | Fully Automated |
| Channel Count | 1 / 2 / 4 Independent Channels |
| Extraction Mode | Cartridge-Based (Column) SPE |
| Flow Rate Control | 0.1–120 mL/min |
| Solvent Capacity | 8 Solvent Types, Each in 500 mL Independent Bottles |
| Sample Loading Volume | 0.1 mL – 20 L |
| Column Compatibility | 1 mL, 3 mL, 6 mL, 12 mL Standard SPE Cartridges |
| Sealing Technology | Patented Pan-Seal Cap Coupling with H-Axis Compression |
| Pressure Range | 0–145 psi (with Adjustable Pressure Threshold & Overpressure Alarm) |
| Mechanical Arm | X/Y/Z/H Four-Axis Robotic Arm (Positioning Accuracy: ±0.01 mm |
| Sample Needle | 316L Stainless Steel with Liquid-Level Tracking & Puncture Sampling |
| Integrated Functions | On-line Drying (N₂ Blow-Down + Anhydrous Na₂SO₄), On-line Concentration (N₂-Assisted), Waste Segregation (3-Channel Effluent Routing), Multi-Mode Needle Cleaning (N₂ Purge, Solvent Rinse, Agitated Rinse) |
| Software | Cloud-Enabled Platform with Remote Monitoring, Scheduled Run Initiation, Audit Trail, Parameter Validation & GLP-Compliant Logging |
| Power Supply | AC 220 V / 110 V ±10%, 50–60 Hz |
| Operating Environment | 0–40 °C, 10–85% RH |
| Dimensions (Main Unit) | 680 × 580 × 670 mm (H×W×D) |
Overview
The Sineo SPE100/200/400 Fully Automated Solid Phase Extraction System is an engineered solution for high-throughput, reproducible sample preparation in environmental, pharmaceutical, food safety, and clinical laboratories. It implements positive-pressure-driven solid-phase extraction using standardized cartridge formats, delivering precise solvent delivery, column conditioning, sample loading, washing, and elution—all under programmable, closed-system control. Unlike gravity- or vacuum-based manual SPE systems, the Sineo platform employs a four-axis (X/Y/Z/H) robotic arm with sub-0.01 mm positioning accuracy and patented pan-seal cap coupling to ensure consistent column compression and pressure integrity up to 145 psi. This architecture enables robust handling of viscous, particulate-laden, or large-volume samples (up to 20 L) without clogging or seal failure—critical for wastewater, serum, or juice matrix applications where filtration or pre-centrifugation is impractical.
Key Features
- Fully modular channel architecture: Supports field-upgradable configurations from 1 to 4 independent processing channels (SPE100 = 1 ch, SPE200 = 2 ch, SPE400 = 4 ch), each with dedicated pressure sensing, flow regulation, and solvent routing.
- High-fidelity fluid handling: Dual-mode sample pump (continuous precision peristaltic + pressure-compensated syringe-assist) delivers stable flow rates from 0.1 to 120 mL/min across wide viscosity ranges; compatible with aggressive solvents including dichloromethane, acetonitrile, and ethyl acetate.
- Intelligent sealing & pressure management: H-axis compression module applies uniform force to SPE cartridges while integrated pressure sensors enable real-time closed-loop control—ensuring consistent bed packing, minimized channeling, and validated method transfer between instruments.
- Integrated on-line sample conditioning: Built-in anhydrous sodium sulfate drying column and N₂ blow-down module allow automated water removal and post-elution concentration without manual transfer—reducing analyte loss, contamination risk, and hands-on time.
- Multi-layer contamination prevention: Three-stage needle cleaning (N₂ purge → solvent rinse → agitated solvent wash), puncture-sampling capability to prevent volatilization, and segregated 3-channel waste collection for acidic, basic, and organic effluents support ISO/IEC 17025-compliant workflow integrity.
Sample Compatibility & Compliance
The system accommodates standard 1 mL, 3 mL, 6 mL, and 12 mL SPE cartridges—including bonded silica, C18, SCX, SAX, and mixed-mode phases—from major vendors (e.g., Waters, Agilent, Thermo Fisher). Custom cartridge adapters are available upon request. It supports EPA Methods 508.1, 525.3, and 8270, as well as ISO 17025 Annex A.2 requirements for documented method validation, instrument qualification (IQ/OQ/PQ), and traceable calibration. All operational parameters—including flow rate, pressure setpoints, dwell times, and solvent selection—are logged with timestamped audit trails compliant with FDA 21 CFR Part 11 (electronic signatures, user access controls, and immutable data archiving). The system meets IEC 61000-6-3 (EMC) and IEC 61010-1 (safety) standards.
Software & Data Management
The embedded Linux-based control software provides intuitive graphical method building, drag-and-drop protocol sequencing, and real-time status visualization (solvent levels, pressure curves, arm position, temperature/humidity). Cloud connectivity enables remote parameter upload/download, firmware updates, and secure technical support via encrypted TLS 1.2 channels. All run logs—including error codes, alarm events, and maintenance timestamps—are exportable in CSV or PDF format. The platform supports scheduled execution (time-triggered or instrument-triggered via TTL handshake), automatic logic validation prior to run initiation, and configurable alert escalation (email/SMS) for critical faults (e.g., low solvent, overpressure, motor stall). Audit trail records include operator ID, action type, timestamp, and pre-/post-value changes—fully aligned with GLP/GMP documentation expectations.
Applications
- Environmental analysis: Extraction of PAHs, PCBs, organochlorine pesticides, and pharmaceutical residues from surface water, wastewater, and soil extracts (EPA 1694, ISO 21676).
- Pharmaceutical QC: Clean-up of plasma, urine, or tissue homogenates prior to LC-MS/MS quantification of APIs and metabolites (USP & Ph. Eur. compliant workflows).
- Food safety testing: Isolation of mycotoxins (aflatoxins, ochratoxin A), veterinary drug residues (tetracyclines, sulfonamides), and pesticide multiresidues from milk, honey, and fruit matrices.
- Clinical toxicology: High-recovery extraction of opioids, benzodiazepines, and stimulants from whole blood or oral fluid with minimal matrix interference.
- Regulatory compliance labs: Method-locked protocols with version-controlled parameters ensure inter-laboratory reproducibility required by CNAS, UKAS, and DAkkS accreditation bodies.
FAQ
What cartridge formats does the Sineo SPE system support?
Standard 1 mL, 3 mL, 6 mL, and 12 mL polypropylene SPE cartridges with fritted beds; custom adapters available for non-standard geometries.
Can the system handle large-volume aqueous samples without clogging?
Yes—the positive-pressure design, abrasion-resistant 316L needle, and high-tolerance pump mechanism enable uninterrupted loading of up to 20 L of turbid or particulate-containing samples (e.g., raw wastewater, centrifuged serum).
Is the software compliant with 21 CFR Part 11 requirements?
Yes—full electronic signature support, role-based access control, immutable audit trails, and automatic backup ensure regulatory readiness for FDA-inspected laboratories.
How is cross-contamination prevented during multi-sample runs?
Through triple-stage needle cleaning (N₂ purge + solvent rinse + agitation), puncture-sampling to avoid headspace exposure, and physically segregated waste routing for incompatible solvents.
Does the system support method development or only routine analysis?
Both—the flexible scripting interface allows full customization of dwell times, flow gradients, solvent sequences, and pressure profiles, enabling optimization of novel SPE chemistries or complex multi-analyte extractions.
