SSI Series III Preparative HPLC Pump

Overview

The SSI Series III Preparative HPLC Pump is a precision-engineered isocratic high-pressure liquid delivery system designed for robust, long-duration operation in preparative chromatography, synthetic chemistry workflows, and process-scale solvent delivery applications. Built upon the proven mechanical architecture of the Series II platform, the Series III incorporates critical enhancements—including a high-speed cam-driven internal priming mechanism and a compact, low-volume diaphragm-type pulse damper—to achieve exceptionally low baseline noise (< 0.1% pressure ripple) and minimal flow pulsation. Its operating principle relies on dual-piston reciprocating motion synchronized via microstepping motor control and digital logic circuitry, ensuring stable volumetric displacement across the full flow range. The integrated piezoresistive pressure transducer provides real-time, calibrated pressure feedback directly to the front-panel LED display—enabling immediate detection of column blockage, filter clogging, or system overpressure events. Designed for continuous-duty cycles under demanding chemical environments, the Series III meets essential operational requirements for GLP-compliant method development, scale-up studies, and automated reaction monitoring.

Key Features

• Dual-material fluid path options: Selectable PEEK or 316L stainless steel pump heads to ensure compatibility with aggressive organic solvents (e.g., DMF, DMSO, dichloromethane) and biocompatible aqueous mobile phases
• Front-panel programmable pressure limits: Independent upper/lower pressure thresholds with automatic shutdown and audible alert to protect columns and system integrity
• On-line self-cleaning function: Integrated bypass valve and reverse-flush capability reduces maintenance frequency and extends seal life without disassembly
• High-resolution flow control: 0.001 mL/min minimum increment with <0.2% RSD repeatability at constant setpoint, validated per ASTM D7892-15 for precision liquid metering
• RS232 serial interface: Enables remote parameter setting, real-time data logging, and integration into centralized lab automation frameworks (e.g., LabVantage, Thermo Fisher SampleManager)
• Modular design: Compatible with SSI gradient modules (e.g., Series III Dual Solvent Selector) for future expansion to binary or ternary solvent systems without hardware replacement

Sample Compatibility & Compliance

The Series III accommodates a broad spectrum of sample matrices and mobile phase chemistries common in pharmaceutical purification, natural product isolation, and catalyst screening. Its wetted components comply with USP Class VI biocompatibility standards when configured with PEEK, and meet FDA 21 CFR Part 11 requirements for electronic record integrity when paired with compliant data acquisition software. Pressure and flow validation protocols align with ISO 17025-accredited calibration practices. The pump supports operation within ICH Q2(R2)-defined analytical method robustness parameters and is routinely deployed in laboratories maintaining GMP Annex 11 and EU GMP Chapter 4 compliance for preparative process documentation.

Software & Data Management

While the Series III operates autonomously via its embedded firmware and front-panel interface, it fully supports external control through ASCII-based RS232 command sets (SSI Protocol v3.2). Third-party chromatography data systems—including Chromeleon CDS, Empower 3, and OpenLab CDS—can initiate run sequences, log pressure/flow timestamps, and trigger alarms via standard SCPI-like syntax. Audit trails capture all parameter changes, including user ID (when authenticated via external login), timestamp, and pre/post-change values—satisfying ALCOA+ data integrity principles. Optional firmware upgrade enables time-stamped event logging to removable USB storage for offline GLP audit review.

Applications

• Preparative HPLC fraction collection under fixed-flow conditions for compound library purification
• Controlled reagent delivery in continuous-flow organic synthesis reactors (e.g., photochemical, cross-coupling, hydrogenation)
• Calibration standard delivery in gravimetric and volumetric verification protocols per ISO/IEC 17025
• Mobile phase supply for multi-column switching systems in 2D-LC and heart-cutting configurations
• Long-term stability testing of chromatographic columns under accelerated pressure cycling (e.g., 10,000+ cycles at 4000 psi)
• Teaching laboratory use for hands-on instruction in pump mechanics, fluid dynamics, and HPLC system troubleshooting

FAQ

What is the maximum recommended operating pressure for PEEK-head configurations?
The PEEK pump head is rated for continuous operation up to 5000 psi (345 bar) at 25°C; pressure derating applies above 40°C per SSI Technical Bulletin TB-047.
Can the Series III be integrated into a 21 CFR Part 11-compliant workflow?
Yes—when used with validated CDS software that implements electronic signatures, audit trail generation, and role-based access control, the Series III satisfies predicate rule requirements for electronic records.
Is pulse damping adjustable or user-serviceable?
The diaphragm-type damper is factory-tuned and sealed; no field adjustment is required or supported. Replacement kits (P/N DAMP-III-PEEK / DAMP-III-SS) are available with recalibration certificate.
Does the pump support gradient formation natively?
No—the Series III is an isocratic pump. Gradient capability requires external integration with SSI Series III Solvent Selection Valves or third-party low-dead-volume mixing modules.
What maintenance intervals are recommended for routine operation?
Seal replacement every 6 months under continuous use; inlet filter cleaning every 2 weeks; annual pressure sensor calibration traceable to NIST standards.