TH-YB416 CloudJing Tianhe Real-Time Fluorescence Quantitative PCR System
| Brand | Tianhe (Shandong Tianhe Instrument Co., Ltd.) |
|---|---|
| Origin | Shandong, China |
| Manufacturer Type | Direct Manufacturer |
| Region of Origin | Domestic (China) |
| Model | TH-YB416 |
| Price | USD 6,700 (approx. based on ¥48,000 at 1 USD ≈ ¥7.15) |
| Sample Capacity | 8 × 0.2 mL tubes |
| Reaction Volume | 5–120 µL |
| Temperature Range | 4–99 °C |
| Max Heating/Cooling Rate | ≥2 °C/s |
| Temperature Uniformity | ≤±0.2 °C |
| Temperature Accuracy | ≤±0.1 °C |
| Excitation Wavelength Range | 400–700 nm |
| Detection Channels | 1 channel (FAM/SYBR Green I) standard |
| Optical Detection | Imported photodetector array |
| Thermal Module | High-performance Peltier (Marlow) with PT1000 sensor and resistive edge compensation |
| Thermal Gradient | None |
| Real-time Data Acquisition | 3-second full-plate read |
| Display | 7-inch HD capacitive touchscreen |
| Noise Level | <45 dB |
| Dimensions (W×L×H) | 290 × 308 × 130 mm |
| Net Weight | ~3 kg |
Overview
The TH-YB416 CloudJing Tianhe Real-Time Fluorescence Quantitative PCR System is a compact, field-deployable nucleic acid amplification platform engineered for rapid, reliable detection of aquatic viral pathogens in aquaculture settings. It operates on the principle of real-time quantitative polymerase chain reaction (qPCR), utilizing sequence-specific primers and hydrolysis probes (e.g., TaqMan) or intercalating dyes (e.g., SYBR Green I) to monitor target DNA amplification in real time via fluorescence emission. The system employs a solid-state thermocycling architecture based on Marlow Peltier modules, coupled with high-precision PT1000 temperature sensing and dynamic resistive edge compensation—enabling accurate thermal control across the entire reaction block. Designed for low-infrastructure environments, it delivers laboratory-grade performance without dependency on centralized infrastructure, making it suitable for on-site diagnostics at hatcheries, processing facilities, and regional disease surveillance units.
Key Features
- Compact, portable form factor (290 × 308 × 130 mm; ~3 kg) optimized for mobile lab deployment and field use.
- Integrated 7-inch HD capacitive touchscreen with intuitive, icon-driven UI—no external PC required for basic operation.
- High-fidelity thermal control: ±0.1 °C accuracy and ≤±0.2 °C uniformity across all 8 wells, validated per ISO 13485-aligned internal calibration protocols.
- Fast thermal ramping: ≥2 °C/s average heating/cooling rate, reducing typical 40-cycle qPCR run time to under 65 minutes.
- Optimized optical path: Maintenance-free LED excitation source (400–700 nm) paired with imported photodetectors ensures stable signal acquisition and low background noise (<45 dB).
- Full-plate fluorescence reading in 3 seconds, minimizing inter-well variability and supporting robust intra-run reproducibility (CV < 2.5% for Cq values).
Sample Compatibility & Compliance
The TH-YB416 accepts standard 0.2 mL transparent PCR tubes and 8-tube strips, compatible with common extraction kits for viral RNA/DNA from gill, hepatopancreas, lymphoid tissue, and water column samples. It supports detection of key aquaculture pathogens including Infectious Hematopoietic Necrosis Virus (IHNV), White Spot Syndrome Virus (WSSV), Taura Syndrome Virus (TSV), and Yellow Head Virus (YHV). The system’s firmware and data handling comply with GLP-relevant audit trail requirements, including timestamped run logs, user authentication, and immutable result export (CSV/PDF). While not FDA 21 CFR Part 11 certified out-of-the-box, its software architecture supports traceable user roles and electronic signature readiness for GMP-aligned validation workflows.
Software & Data Management
Preloaded CloudJing qPCR Analysis Software provides real-time amplification curve visualization, automatic baseline and threshold setting, Cq calculation, and binary positive/negative classification per well. It includes built-in templates for common aquatic virus assays and allows custom assay configuration (melting curve analysis, multi-step cycling, passive reference normalization). Data export is supported via USB 2.0 interface in CSV, Excel, and PDF formats. Raw fluorescence intensity files (.fam, .vic) are stored with metadata (operator ID, date/time, protocol name, instrument serial), enabling retrospective reanalysis and integration into LIMS environments.
Applications
- Routine surveillance of viral pathogens in shrimp, tilapia, salmonid, and crustacean farms.
- Emergency response diagnostics during disease outbreaks, supporting rapid containment decisions.
- Validation of biosecurity protocols and post-treatment clearance testing.
- Research applications in viral load quantification, host-pathogen interaction studies, and antiviral efficacy assessment.
- Regulatory compliance testing for national aquaculture health certification programs (e.g., OIE-listed disease reporting).
FAQ
Is the TH-YB416 suitable for RNA virus detection?
Yes—the system supports reverse transcription qPCR (RT-qPCR) when used with commercially available RT master mixes and appropriate controls.
Can the instrument be validated for regulatory submission?
Yes—its thermal and optical performance parameters meet ISO 20387:2018 Annex B criteria for molecular diagnostic devices; IQ/OQ documentation packages are available upon request.
Does it support multiplex detection?
The base configuration includes one detection channel (FAM/SYBR Green I); dual-channel capability (FAM + VIC/HEX) is available as a factory-configured option.
What maintenance is required?
No routine optical alignment or lamp replacement is needed; only periodic verification of thermal calibration using NIST-traceable reference standards is recommended annually.
Is remote monitoring or cloud connectivity supported?
Local Wi-Fi and Bluetooth are not integrated; however, exported data files are compatible with third-party cloud-based analytics platforms via secure USB transfer.
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