Thermo Fisher TriPlus 100 LS Multifunctional Autosampler

Overview

The Thermo Fisher TriPlus 100 LS Multifunctional Autosampler is an engineered solution for high-integrity, unattended liquid sample introduction in gas chromatography (GC) workflows. Designed as a modular extension of the TriPlus RSH platform architecture, it implements precise syringe-based liquid injection with programmable positioning, pressure-assisted needle penetration, and integrated solvent management—enabling robust operation across diverse matrix types, including viscous, particulate-laden, or thermally labile samples. Its core mechanism relies on stepper-motor-driven precision mechanics combined with electronic pneumatic control (EPC) for reproducible injection volume delivery (typically 0.1–100 µL), minimizing carryover and maximizing method transferability between instruments. The system is not a standalone analyzer but a validated front-end automation component intended for integration with Thermo Scientific™ Trace™ GC, ISQ™ mass spectrometers, or third-party GC systems compliant with standard RS-232/USB/ethernet command protocols.

Key Features

• Modular architecture supporting field-upgradable configurations: Double Pro dual-GC mode, Confirmation mode, and optional hardware add-ons including vortexer, stirrer, refrigerated sample tray (4–40 °C), and temperature-controlled drawer (ambient to 60 °C)
• High-capacity sample storage: Up to 162 positions (12 × 13.5 mL vials) or 324 positions (24 × 2 mL vials); expandable via stackable racks
• Extended solvent management: Dual solvent stations (up to 4 solvents total) with configurable wash cycles, waste reservoir capacity up to 1 L per station
• Method-driven liquid injection: Eight fully customizable, instrument-method-linked injection sequences accessible directly from Chromeleon CDS software—no manual macro scripting required
• Needle path optimization: Adaptive vertical/horizontal motion control minimizes vial septum damage and ensures consistent penetration depth across varying vial geometries and septum hardness
• Compliance-ready design: Supports audit trail logging, electronic signatures, and user access levels per FDA 21 CFR Part 11 requirements when operated with Chromeleon 7.3 or later

Sample Compatibility & Compliance

The TriPlus 100 LS accommodates standard GC vial formats (12 mm × 32 mm, 18 mm × 70 mm, crimp-top, screw-cap) and supports derivatized, aqueous, organic, and high-salt matrices without requiring hardware modification. It meets ISO/IEC 17025:2017 recommendations for automated sampling integrity and is routinely deployed in GLP-compliant environmental testing labs (e.g., EPA Method 502.2, 8021B), pharmaceutical QC (USP , ICH Q2(R2)), and food safety applications (AOAC 2007.01). All firmware and driver updates are traceable through Thermo Fisher’s validated release documentation package.

Software & Data Management

Fully native integration with Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) v7.3+ enables end-to-end method definition, sequence building, real-time status monitoring, and post-run reporting. Chrom-Card functionality provides one-click launch of preconfigured analytical workflows—including calibration curves, internal standard correction, and peak purity assessment—without modifying instrument methods. Audit trails record all user actions, parameter changes, and system events with timestamped, non-erasable entries. Data export conforms to ASTM E1998-22 (Standard Practice for Chromatographic Data Exchange) and supports .cdf, .axd, and .csv formats for LIMS interfacing.

Applications

• Environmental analysis: VOCs in groundwater (EPA 8260D), pesticides in soil extracts, hydrocarbon fingerprinting in crude oil
• Pharmaceutical residual solvents: Class 1–3 solvent quantification per ICH Q3C guidelines using headspace-GC/FID or GC/MS
• Food & beverage: Ethanol profiling in wines, flavor compound screening in dairy products, mycotoxin detection in cereals
• Polymer additives: Antioxidants, plasticizers, and UV stabilizers in polyolefin extracts analyzed by GC-ECD or GC-MS/MS
• Forensic toxicology: Quantitative determination of volatile drugs-of-abuse (e.g., ethanol, methanol, isopropanol) in blood headspace samples

FAQ

Is the TriPlus 100 LS compatible with non-Thermo GC systems?
Yes—it supports industry-standard ASCII command sets over RS-232, USB virtual COM, or Ethernet TCP/IP, enabling integration with Agilent, Shimadzu, and PerkinElmer GC platforms via documented protocol mapping.
What is the maximum allowable syringe volume?
The standard configuration uses a 10 µL or 50 µL fixed-loop or variable-volume syringe; custom 100 µL syringes are available under OEM agreement with mechanical recalibration.
Does it support cold injection techniques such as PTV or CIS?
No—the TriPlus 100 LS is optimized for split/splitless liquid injection only; cold-on-column or programmed temperature vaporizer (PTV) capabilities require the TriPlus RSH with dedicated PTV module.
Can wash solvent selection be triggered dynamically within a sequence?
Yes—Chromeleon allows per-injection solvent assignment (e.g., rinse A after polar analytes, rinse B after lipid-rich extracts) with automatic valve switching and dwell time control.
Is preventive maintenance documentation included?
Yes—Thermo Fisher provides a complete PM kit (PN 08 020251) with calibrated tools, replacement septa, syringe seals, and step-by-step SOPs aligned with ISO 9001:2015 service verification requirements.