TSIKER Syn-CHY-192 Automated Single-Stranded Nucleic Acid Purification System for DNA/RNA Synthesis
| Brand | TSIKER |
|---|---|
| Origin | Beijing, China |
| Manufacturer | Yes |
| Type | Domestic |
| Model | Syn-CHY-192 |
| Pricing | Upon Request |
Overview
The TSIKER Syn-CHY-192 is an automated, high-throughput purification system engineered specifically for post-synthesis processing of synthetic oligonucleotides—including DNA primers, probes, and RNA sequences—generated on parallel 192-channel DNA synthesizers (e.g., Syn-192P/B). It implements a precisely timed, multi-step solid-phase purification workflow comprising DMT deblocking, desalting, and post-ammonolysis deprotection, all executed under controlled fluidic conditions. The system operates on the principle of sequential reagent delivery through chemically inert PTFE fluidic pathways and precision-engineered PEEK/316L stainless steel dispensing manifolds, enabling reproducible column-based elution and waste evacuation. Designed for integration into GMP-aligned oligonucleotide manufacturing workflows or high-volume academic core facilities, the Syn-CHY-192 delivers deterministic purification performance without manual intervention, reducing operator variability and contamination risk.
Key Features
- High-throughput capacity: Processes two full 96-well plates (192 columns) in ≤12 minutes—optimized for compatibility with TSIKER’s 192-channel synthesizers.
- 8-channel parallel reagent dispensing: Electromagnetically actuated 1-to-8 valve manifold ensures accurate, synchronized delivery of up to five distinct reagents (e.g., deblocking acid, wash solvents, ammonia-compatible deprotection buffers).
- Chemically resistant fluidics: All wetted components—including tubing, needle plates, and column adapters—are constructed from PTFE and medical-grade PEEK; dispensing needles are seamless 316L stainless steel capillaries with positional accuracy <±0.15 mm and laminar, columnar liquid ejection.
- Dual independent chamber control: Left and right plate chambers operate with isolated vacuum and pressure regulation, preventing cross-contamination and enabling differential protocol execution per plate.
- Integrated industrial tablet HMI: Preloaded with TSIKER PurifyControl™ firmware; supports protocol import/export, real-time status monitoring, event logging, and audit-ready operation records.
- Compact benchtop footprint: Dimensions 900 × 480 × 430 mm (L×W×H); weight 55 kg; designed for standard laboratory environments with AC220V power supply.
Sample Compatibility & Compliance
The Syn-CHY-192 accommodates standard 96-well synthesis plates with CPG- or polystyrene-based solid supports (pore sizes 500–2000 Å), compatible with phosphoramidite chemistry using common protecting groups (e.g., DMT, β-cyanoethyl, acetyl, phenoxyacetyl). It supports purification of DNA (15–120 nt), modified DNA (e.g., biotin-, fluorescein-, or amino-modified), and unmodified RNA (up to 60 nt) following standard ammonolysis protocols. While not certified to ISO 13485 or FDA 21 CFR Part 11 out-of-the-box, the system’s firmware architecture supports optional validation packages—including electronic signature capability, user role-based access control, and immutable audit trails—for laboratories operating under GLP, GMP, or ISO/IEC 17025 frameworks. All reagent contact materials comply with USP Class VI biocompatibility standards.
Software & Data Management
The embedded PurifyControl™ software provides intuitive protocol configuration via drag-and-drop step sequencing, with adjustable parameters for dwell time, vacuum level, flow rate, and rinse cycles. Each run generates a timestamped log file (.csv) containing instrument status, valve actuation events, pressure readings, and error flags—exportable via USB or network interface. Optional TSIKER LabLink™ middleware enables direct integration with LIMS platforms (e.g., Thermo Fisher SampleManager, LabVantage) and supports ASTM E1384-compliant data exchange. Firmware updates are delivered via signed OTA packages; configuration backups adhere to ICH M5 archival requirements.
Applications
- High-volume primer production for qPCR, NGS library prep, and CRISPR gRNA synthesis.
- Post-synthesis clean-up of modified oligonucleotides used in diagnostic probe manufacturing.
- Automated purification in academic core facilities supporting >500 synthesis runs/month.
- Process development and scale-down studies for cGMP oligonucleotide API manufacturing.
- Integration into end-to-end synthetic biology workflows—from gene fragment assembly to functional validation.
FAQ
What synthesis instruments is the Syn-CHY-192 designed to interface with?
It is mechanically and protocol-optimized for TSIKER Syn-192P/B synthesizers but accepts standard 96-well plates compatible with ABI, BioAutomation, or other third-party 192-channel platforms via adapter kits.
Can the system handle RNA synthesis purification after methylamine/ammonia cleavage?
Yes—it supports post-cleavage purification of RNA oligos when used with compatible deprotection buffers and low-temperature protocols (≤25°C ambient).
Is remote monitoring or cloud-based data backup supported?
Local network connectivity enables SNMP-based health monitoring; cloud backup requires on-premise IT infrastructure integration via TSIKER LabLink™ gateway.
Does the system include validation documentation for regulated environments?
Factory-installed IQ/OQ documentation is available upon request; PQ support and 21 CFR Part 11 compliance modules require site-specific qualification services.
What maintenance intervals are recommended for fluidic components?
PTFE tubing and PEEK manifolds are rated for ≥10,000 cycles; 316L needles require visual inspection every 500 runs and replacement every 2,000 runs under typical usage.
