UV-Vis Detector by Agela Technologies
| Brand | Agela Technologies |
|---|---|
| Origin | Tianjin, China |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Domestic (China) |
| Model | UV-Vis Detector |
| Pricing | Available Upon Request |
Overview
The Agela UV-Vis Detector is a high-stability, dual-wavelength absorbance detector engineered for integration into liquid chromatography (LC) and preparative HPLC systems. It operates on the principle of Beer–Lambert absorption spectroscopy, measuring analyte concentration via optical density at user-selectable wavelengths across the 190–800 nm spectral range. Designed for routine QC/QA laboratories and method development environments, this detector delivers consistent baseline stability and low drift—critical for quantitative accuracy in gradient elution and fraction collection workflows. Its optical path incorporates a precision quartz flow cell with optimized light path geometry to minimize scattering artifacts and enhance signal-to-noise ratio.
Key Features
- Dual-channel photometric architecture enabling simultaneous detection at two independent wavelengths (e.g., 254 nm and 280 nm), supporting ratiometric analysis and selective compound tracking in complex matrices.
- Contamination-resistant flow cell design featuring a self-draining chamber geometry and bubble-tolerant optical window alignment—reducing maintenance frequency and downtime during extended unattended operation.
- High-intensity deuterium/tungsten dual-lamp source with automatic lamp switching and real-time intensity monitoring to ensure wavelength accuracy and photometric reproducibility over time.
- Integrated analog and digital output interfaces (0–1 V, RS-232, USB) compatible with major LC system controllers and third-party data acquisition platforms.
- Front-panel keypad and LCD display for local parameter configuration—including wavelength selection, bandwidth, response time, and zero/baseline adjustment—without requiring external software.
Sample Compatibility & Compliance
This detector is compatible with standard stainless-steel and PEEK capillary tubing (ID 0.13–0.25 mm), common LC solvents (including acetonitrile, methanol, water, and buffered mobile phases), and typical analytical and semi-preparative column dimensions (2.1–21.2 mm I.D.). It meets mechanical and electrical safety requirements per GB/T 18268.1–2010 (equivalent to IEC 61326-1) for laboratory equipment. While not certified for GMP production environments, its design supports GLP-compliant documentation practices when paired with validated chromatography data systems (CDS) that provide audit trails, electronic signatures, and 21 CFR Part 11–compliant data handling.
Software & Data Management
The detector operates as a standalone analog signal generator but is fully interoperable with industry-standard CDS platforms including Agilent OpenLab, Waters Empower, and Shimadzu LabSolutions. Raw absorbance signals are acquired at up to 20 Hz sampling rate and digitized with 16-bit resolution. Time-stamped analog outputs support synchronized fraction triggering in preparative systems. For method transfer and instrument qualification, Agela provides IQ/OQ documentation templates aligned with ASTM E2500 and USP guidelines. Firmware updates are delivered via USB flash drive and include version-controlled release notes traceable to internal change control records.
Applications
- Quantitative determination of pharmaceutical actives and impurities in HPLC assays per USP monographs.
- Monitoring peptide synthesis purity and cleavage efficiency in process analytical technology (PAT) setups.
- Real-time UV-triggered fraction collection in natural product isolation workflows.
- Stability-indicating method development for degradation product profiling under ICH Q1/Q5 conditions.
- Teaching and research applications in university analytical chemistry labs where dual-wavelength validation enhances student understanding of chromophore behavior.
FAQ
Is this detector compatible with UHPLC systems?
Yes—it supports maximum flow rates up to 10 mL/min and pressure ratings up to 40 MPa when used with appropriate flow cells; however, optimal performance at sub-2-µm particle columns requires optional low-dead-volume cell configurations.
Does it support wavelength scanning or spectral acquisition?
No—this is a fixed-wavelength or dual-fixed-wavelength detector; it does not perform full-spectrum scanning. For spectral characterization, a diode array detector (DAD) is recommended.
Can the analog output trigger external fraction collectors?
Yes—both channel A and channel B analog outputs can be configured to generate TTL-compatible trigger pulses based on user-defined absorbance thresholds.
What is the typical baseline noise specification?
RMS noise is ≤0.5 × 10⁻⁵ AU at 254 nm with a 1-second time constant using a 10-mm pathlength flow cell and deionized water as mobile phase.
Is firmware upgrade support available after purchase?
Yes—Agela provides free firmware updates for the lifetime of the hardware platform, distributed through authorized service partners in North America, EMEA, and APAC regions.
