Varian Cary 400 UV-Vis Spectrophotometer (Refurbished)
| Brand | Varian |
|---|---|
| Origin | USA |
| Model | Cary 400 |
| Product Category | Spectroscopy |
| Instrument Age | 3–4 years |
| Warranty | 6 months |
| Configuration | Complete system with standard accessories |
| Compliance | Fully tested per ASTM E275 and ISO 6223 standards for photometric accuracy and wavelength calibration |
Overview
The Varian Cary 400 UV-Vis Spectrophotometer is a high-performance, double-beam scanning spectrophotometer engineered for precision absorbance, transmittance, and reflectance measurements across the ultraviolet and visible spectral range (190–1100 nm). Based on a Czerny-Turner monochromator architecture with a high-efficiency holographic grating and low-noise silicon photodiode detector, the instrument delivers excellent photometric linearity (< ±0.002 A), wavelength accuracy (±0.3 nm), and baseline stability (< 0.0005 A/h). This refurbished unit has undergone full functional validation at SpectraLab Scientific’s ISO/IEC 17025-accredited calibration facility, including wavelength verification using holmium oxide and didymium filters, photometric accuracy assessment with neutral density filters, and stray light evaluation per ASTM E275. All optical alignment, lamp intensity profiling, and electronic diagnostics were completed to meet original Varian factory specifications.
Key Features
- Double-beam optical design with real-time reference beam correction for enhanced stability against source drift and environmental fluctuations
- High-intensity deuterium and tungsten-halogen lamps with automatic lamp switching and optimized lifetime management
- Scan speed adjustable from 10 to 2400 nm/min, supporting both rapid kinetic profiling and high-resolution spectral acquisition
- Integrated 8-position sample changer compatible with 10 mm pathlength cuvettes (standard) and optional micro-volume adapters
- Robust mechanical construction with temperature-stabilized optics housing to minimize thermal drift during extended measurements
- Comprehensive diagnostic suite accessible via front-panel interface, including lamp energy monitoring, slit width verification, and grating positioning feedback
Sample Compatibility & Compliance
The Cary 400 accommodates standard quartz, glass, or plastic cuvettes (10 × 10 mm cross-section), as well as flow cells, integrating sphere attachments, and solid-sample holders for diffuse reflectance analysis. It supports liquid, solution-based, thin-film, and powdered samples in routine QC, R&D, and academic applications. The system complies with key regulatory requirements for analytical instrumentation: wavelength and photometric performance validated per ASTM E275 (Standard Test Methods for Absorption Spectrophotometers) and ISO 6223 (Molecular absorption spectrometry — Calibration and verification of spectrophotometers). All refurbished units include documented traceable calibration records aligned with ISO/IEC 17025 principles and are suitable for GLP environments where audit-ready documentation is required.
Software & Data Management
The instrument operates with Cary WinUV software (v6.1 or later), providing full control over acquisition parameters, quantitative analysis (single/multi-wavelength, kinetics, scanning), and method storage. Data export supports CSV, ASCII, and JCAMP-DX formats for seamless integration into LIMS or third-party statistical platforms (e.g., JMP, MATLAB, Python pandas). Audit trail functionality meets FDA 21 CFR Part 11 requirements when deployed with appropriate network configuration and user access controls. Software licenses are included and fully transferable; no dongles or hardware keys are required. Raw spectral data files retain full metadata (date/time stamp, operator ID, instrument serial number, lamp hours, and calibration status flags).
Applications
- Quantitative determination of nucleic acids and proteins (A260/A280 ratios, Bradford/Lowry assays)
- Pharmaceutical dissolution testing and content uniformity analysis per USP <711>
- Water quality monitoring (nitrate, phosphate, COD, heavy metal complexes)
- Polymer characterization (conjugation degree, degradation kinetics)
- Enzyme kinetics (NADH/NADPH oxidation/reduction at 340 nm)
- Colorimetric assay development and validation in clinical and food safety laboratories
FAQ
Is this a factory-refurbished unit or dealer-refurbished?
This Cary 400 has been professionally refurbished by SpectraLab Scientific, an ISO/IEC 17025-accredited service provider specializing in analytical instrumentation. Refurbishment includes full optical recalibration, mechanical inspection, firmware update, and comprehensive functional testing.
Does the 6-month warranty cover parts and labor?
Yes—the warranty includes all parts, labor, and return shipping for covered failures. Extended service plans (12–24 months) with priority response and remote diagnostics support are available upon request.
Can the instrument be configured for GMP-compliant environments?
Yes—when paired with validated Cary WinUV software, user access controls, and documented calibration history, the system supports 21 CFR Part 11 compliance. Full IQ/OQ documentation packages can be provided for installation qualification.
Are original manuals and software installation media included?
Yes—digital copies of the Varian Cary 400 User Manual, Service Manual, and WinUV Installation Guide are supplied. Physical media is not included, but licensed software installers are provided via secure download link with activation credentials.
What is the typical lead time for delivery after order confirmation?
Most units ship within 3–5 business days from order confirmation, as all refurbished Cary 400 systems are held in ready-to-ship inventory with pre-installed and verified configurations.
