Yoke L5 IoT-Enabled Dual-Beam UV-Vis Spectrophotometer
| Brand | Yoke |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Regional Classification | Domestic (China) |
| Model | L5 |
| Instrument Architecture | Dual-Beam |
| Detector Type | Photodiode Array (PDA) |
| Wavelength Range | 190–1100 nm (UV-Vis) |
| Wavelength Accuracy | ±1 nm |
| Wavelength Repeatability | ≤0.5 nm |
| Spectral Bandwidth | 2 nm |
| Stray Light | ≤0.5% T at 420 nm (NaNO₂) |
| Automation Level | Automatic Wavelength Selection & Lamp Switching |
| Data Storage Capacity | 500 spectra, 30 calibration curves |
| Connectivity | USB 2.0, Wi-Fi 802.11 b/g/n, Bluetooth 4.2 |
| Software Compatibility | Yokelab PC Suite (optional), Excel-compatible CSV export |
Overview
The Yoke L5 IoT-Enabled Dual-Beam UV-Vis Spectrophotometer is an engineered solution for routine and research-grade absorbance measurements across the ultraviolet, visible, and near-infrared spectral regions (190–1100 nm). Based on dual-beam optical architecture, it continuously compares sample and reference beam intensities in real time—minimizing errors from source drift, detector instability, or environmental fluctuations. The integrated photodiode array (PDA) detector enables rapid full-spectrum acquisition without mechanical scanning, supporting high-throughput applications while maintaining consistent photometric linearity and signal-to-noise ratio. Designed for laboratory environments requiring regulatory traceability and operational continuity, the L5 complies with core performance benchmarks aligned with ISO 9001 quality management systems and supports audit-ready data handling under GLP-aligned workflows.
Key Features
- High-resolution 7-inch capacitive touchscreen interface with intuitive icon-driven navigation—no keyboard or mouse required.
- Dual-beam optical path with automatic deuterium/tungsten lamp switching ensures seamless coverage across UV and Vis ranges without manual intervention.
- IoT-ready connectivity stack: embedded Wi-Fi (802.11 b/g/n) and Bluetooth 4.2 enable secure instrument pairing with mobile devices, cloud-based instrument status monitoring, and wireless thermal printing.
- Onboard storage capacity accommodates up to 500 spectral scans and 30 user-defined calibration curves—retained even after power cycling.
- USB 2.0 host port supports direct data export to FAT32-formatted USB drives; exported files are saved as UTF-8 encoded CSV, fully compatible with Microsoft Excel, MATLAB, Python (pandas), and third-party LIMS platforms.
- Real-time wavelength calibration verification using internal reference peaks ensures long-term spectral fidelity without external standards.
Sample Compatibility & Compliance
The L5 accepts standard 10 mm square cuvettes (glass, quartz, or plastic) and accommodates optional accessories including micro-volume adapters (down to 50 µL), temperature-controlled holders (±0.1 °C stability), and fiber-optic probes for remote or in-line measurements. Its optical design meets ASTM E275–22 (Standard Practices for Describing and Measuring Performance of UV-Vis Spectrophotometers) for photometric accuracy and stray light assessment. While not certified for FDA 21 CFR Part 11 out-of-the-box, the Yokelab PC Suite (v3.2+) provides optional electronic signature support, audit trail logging, and role-based access control—enabling compliance with GxP environments when deployed with validated procedures.
Software & Data Management
The optional Yokelab PC Suite offers comprehensive spectral analysis capabilities: kinetic time-series plotting, multi-wavelength quantitation, nucleic acid/protein concentration calculation (A260/A280 ratios), derivative spectroscopy, spectral overlay with baseline correction, and integration of peak areas. All instrument settings, measurement logs, and raw absorbance/transmittance data are timestamped and stored with metadata (operator ID, date/time, method name, cuvette pathlength). Exported CSV files include column headers compliant with ASTM E1421–21 (Standard Guide for Data Exchange in Spectroscopy), facilitating interoperability with enterprise analytics tools.
Applications
- Routine QC testing of pharmaceutical excipients and active ingredients per USP and EP 2.2.25.
- Environmental water analysis (e.g., nitrate, phosphate, COD estimation) following APHA Standard Methods 4500.
- Food and beverage quality control: color index evaluation (CIE L*a*b* conversion), antioxidant capacity assays (FRAP, DPPH).
- Academic teaching labs: Beer–Lambert law validation, enzyme kinetics (e.g., LDH, ALP), and equilibrium constant determination.
- Materials science: bandgap estimation of semiconductor nanoparticles via Tauc plot analysis.
FAQ
Does the L5 support GLP-compliant data integrity requirements?
Yes—when used with Yokelab PC Suite v3.2+ and configured with password-protected user roles, session logging, and immutable audit trails, it satisfies core GLP documentation criteria for instrument-generated data.
Can I perform kinetic measurements without connecting to a computer?
No—kinetic mode requires Yokelab PC Suite for time-resolved data acquisition and curve fitting; onboard functionality supports only single-point, fixed-wavelength, or full-spectrum snapshot measurements.
Is quartz cuvette compatibility confirmed across the full 190–1100 nm range?
Yes—standard 10 mm quartz cuvettes transmit effectively from 190 nm through 2500 nm; the L5’s optical path is optimized for use with such cells in both UV and NIR sub-ranges.
What is the maximum sampling rate during wavelength scanning?
At 2 nm spectral bandwidth and 1 nm step resolution, the PDA enables full 190–1100 nm scans in ≤1.8 seconds with <0.5% photometric noise at 1.0 A.
How is wavelength accuracy verified during routine operation?
The system performs automated wavelength calibration checks using built-in holmium oxide and didymium glass filters (optional accessory); verification reports are generated and stored with each calibration event.
