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| Brand | LifeCanvas Technologies |
|---|---|
| Origin | USA |
| Model | SmartSPIM |
| Illumination | Dual-sided scanned light sheet (US Patent No. US10989661B2) |
| Sample Chamber Dimensions (Standard) | 25 mm × 25 mm × 20 mm (W × L × H) |
| Extended Chamber | 40 mm × 65 mm × 20 mm |
| Objective NA (Illumination) | 0.125 |
| Tube Lens | 180 mm EFL, achromatic wide-spectrum correction |
| Standard Detection Objective | 3.6×, NA = 0.3, WD = 18 mm, pixel size = 1.8 µm × 1.8 µm (with 50 mm EFL tube lens) |
| Optional Objectives | 1.63×, 9×, 15×, 22× |
| Axial Resolution (3.6×) | PSF<sub>z</sub> = 3.2–4.0 µm |
| Field of View (3.6×) | 3650 µm |
| Lateral Sampling (3.6×) | 1.8 µm/pixel |
| Z-step | 4 µm |
| Max Frame Rate | 20 FPS |
| Whole Mouse Brain Imaging Time (Single Channel) | ≤30 min |
| Camera | 2048 × 2048 sCMOS, rolling shutter synchronized with light sheet scan, QE = 82%, max 80 fps |
| Laser Lines (Standard) | 488 nm, 561 nm, 639 nm (3 lines) |
| Optional Lasers | 405 nm, 445 nm, 690 nm, 785 nm (up to 6 total) |
| Acquisition Workstation | Intel Core i7-10700K (3.8 GHz), 32 GB DDR4 quad-channel RAM, onboard 1 GbE/10 GbE NIC, Windows 10 Pro, 4K display |
| Software | SmartSPIM Acquisition & Post-processing Suite (real-time tile correction, block alignment, stripe artifact removal, GPU-accelerated 3D reconstruction) |
| Analysis Workstation | AMD Ryzen Threadripper 3960X (24-core), 128 GB DDR4 quad-channel RAM, 32 TB RAID 0 storage (4 × 8 TB, 7200 RPM), Windows 10 Pro |
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