Beijing Liuyi DYCZ-MINI2 Dual-Plate Vertical Electrophoresis Apparatus

Overview

The Beijing Liuyi DYCZ-MINI2 Dual-Plate Vertical Electrophoresis Apparatus is a compact, benchtop-compatible system engineered for high-reproducibility vertical slab gel electrophoresis—including SDS-PAGE, native PAGE, and isoelectric focusing (IEF). Its dual-plate architecture enables simultaneous casting and electrophoretic separation of one or two gels in a single run, supporting both hand-poured and pre-cast polyacrylamide formats. The apparatus operates on the principle of electrophoretic mobility under a controlled electric field, where charged biomolecules (e.g., proteins or nucleic acids) migrate through a porous polyacrylamide matrix at rates determined by molecular weight, charge, and conformation. Designed for routine laboratory use in academic research, quality control, and teaching environments, the DYCZ-MINI2 delivers consistent field homogeneity and thermal stability across the gel surface—critical for sharp band resolution and inter-run comparability.

Key Features

• High-purity platinum wire electrodes ensure low-resistance current transmission and long-term corrosion resistance, minimizing voltage drop and electrochemical side reactions during extended runs.
• Integrated edge-sealing spacers are permanently affixed to the long glass plate, eliminating lateral misalignment and significantly reducing gel leakage during polymerization and electrophoresis.
• Cam-lock gel casting frame provides tactile, repeatable alignment of short and long glass plates on any level surface—no tools or calibration required.
• Proprietary polymer comb design incorporates internal ridges that promote uniform acrylamide polymerization across all wells, preventing “smiling” or uneven gel front formation.
• Reinforced long glass plates (10.1 × 8.2 cm) feature increased thickness for enhanced mechanical rigidity, reducing breakage risk during handling, washing, and staining procedures.
• Integrated sample loading guide ensures precise pipette tip placement over each well, minimizing skipped lanes or double-loading errors—especially critical for low-volume, high-value samples.

Sample Compatibility & Compliance

The DYCZ-MINI2 supports standard vertical electrophoresis workflows compliant with widely adopted life science protocols, including those referenced in ISO 13843:2017 (molecular biology—electrophoresis methods), ASTM E2917-22 (standard guide for electrophoretic separation of proteins), and common USP recommendations for gel-based analytical method validation. It accommodates both conventional hand-cast gels (8.3 × 7.3 cm, 1.0 mm thick) and commercially available pre-cast mini-gels (8.6 × 6.8 cm), enabling seamless integration into existing lab workflows. The system’s 200 V maximum output is compatible with standard power supplies used in IEF and SDS-PAGE applications requiring moderate field strength (typically 10–20 V/cm). All wetted components—including glass plates, combs, and sealing gaskets—are autoclavable or chemically resistant to common electrophoresis reagents (e.g., SDS, TEMED, APS, acetic acid, methanol).

Software & Data Management

As a standalone electrophoresis apparatus without integrated imaging or digital readout, the DYCZ-MINI2 does not include proprietary software or data acquisition modules. However, its standardized gel dimensions and compatibility with third-party documentation systems—including GelDoc™ platforms (Bio-Rad), Azure Sapphire™ scanners, and ImageLab™ or AlphaView™ analysis software—facilitate traceable image capture, densitometric quantification, and GLP-aligned reporting. When used in regulated environments (e.g., QC labs operating under GMP or ISO/IEC 17025), users may implement manual logbooks or LIMS-integrated run records specifying voltage, run time, gel composition, and ambient temperature—satisfying audit requirements for instrument usage history and method adherence.

Applications

• SDS-PAGE for protein molecular weight estimation and purity assessment in recombinant expression studies.
• Native PAGE for analyzing protein oligomeric states, enzyme activity profiling, and complex assembly under non-denaturing conditions.
• Isoelectric focusing (IEF) for high-resolution pI determination of protein isoforms, post-translational modifications, and charge heterogeneity screening.
• Two-dimensional electrophoresis (2-DE) first-dimension IEF followed by second-dimension SDS-PAGE, using compatible IPG strips and transfer protocols.
• Teaching laboratories requiring robust, intuitive hardware for undergraduate biochemistry and molecular biology instruction.

FAQ

Is the DYCZ-MINI2 compatible with common pre-cast gels from major suppliers?

Yes—the chamber accommodates standard mini-gel dimensions (8.6 × 6.8 cm), aligning with formats from Bio-Rad, Thermo Fisher, and Lonza.
Can I perform IEF with immobilized pH gradients (IPG) using this system?

Yes—the vertical orientation, stable electrode contact, and uniform buffer circulation support IPG strip rehydration and focusing runs when paired with appropriate IEF buffers and power supply settings.
What safety certifications does the apparatus carry?

The unit complies with GB/T 14710–2009 (Environmental Adaptability of Medical Electrical Equipment) and meets CE-marking requirements for electrical safety (EN 61010-1) when used with certified external power supplies.
Are replacement parts (e.g., combs, glass plates, spacers) available separately?

Yes—Beijing Liuyi offers OEM-matched consumables and accessories, including 10- and 15-tooth combs, 0.75/1.0/1.5 mm spacers, and reinforced glass plate sets.
Does the system support temperature-controlled electrophoresis?

No—the DYCZ-MINI2 lacks active cooling or heating elements; however, its low-profile design and acrylic chamber minimize Joule heating, and ambient temperature control (e.g., cold room operation) is recommended for high-voltage or prolonged runs.