Anyeep Cassitrap 120K Quadrupole–Fourier Transform Electrostatic Trap Gas Chromatography Mass Spectrometer

Overview

The Anyeep Cassitrap 120K is a hybrid gas chromatography mass spectrometry (GC-MS) system integrating a robust quadrupole mass filter with a high-field electrostatic ion trap operating in Fourier transform (FT) detection mode. Unlike conventional time-of-flight (TOF) or orbitrap-based platforms, the Cassitrap architecture employs a static electric field to radially confine ions within a three-dimensional trap, enabling long transient acquisition times and ultra-high mass resolving power without magnetic components or RF-driven orbital dynamics. This design delivers simultaneous high sensitivity, sub-ppm mass accuracy, and structural elucidation capability—particularly valuable for non-targeted screening, metabolite identification, and trace-level environmental contaminant analysis. The system is engineered for seamless integration with standard capillary GC inlets and supports both electron ionization (EI) and optional chemical ionization (CI) sources.

Key Features

• Hybrid dual-mode acquisition: Switchable quadrupole scanning (for rapid quantitation) and FT-electrostatic trap acquisition (for high-resolution, high-mass-accuracy identification)
• Mass resolution exceeding 120,000 FWHM at m/z 131 under routine 1.5 Hz spectral acquisition—enabling baseline separation of isobaric interferences in complex matrices
• Sub-2 ppm mass accuracy in FT mode with internal calibration, supporting confident elemental composition assignment per IUPAC guidelines
• Ultra-fast GC oven ramp rates up to 250 °C/min (with fast-heating oven option), reducing total run time without compromising peak capacity or resolution
• Gas-phase pressure control stability of ±0.001 psi across the entire ion path—critical for maintaining consistent ion transmission and minimizing mass drift during extended acquisitions
• Dual-detector architecture: Electron multiplier for quadrupole mode; dedicated FT detection electronics with phase-sensitive signal processing for coherent ion signal demodulation

Sample Compatibility & Compliance

The Cassitrap 120K accepts volatile and semi-volatile organic compounds eluting from standard 0.18–0.32 mm ID fused-silica capillary columns. It is compatible with common derivatization protocols (e.g., silylation, acylation) for polar analytes such as organic acids, sugars, and amino acids. The system meets essential regulatory requirements for laboratory use in regulated environments: hardware and firmware support audit-trail-enabled data acquisition (aligned with FDA 21 CFR Part 11 principles), and software architecture allows configuration for GLP/GMP-compliant workflows. While not pre-certified to ISO/IEC 17025, its performance specifications align with ASTM E2967 (Standard Guide for High-Resolution GC-MS) and USP validation parameters for identification and quantitation.

Software & Data Management

The proprietary Anyeep MassAnalyzer Suite provides integrated instrument control, real-time spectral visualization, and post-acquisition processing—including automated peak deconvolution, library matching (NIST/EPA/WHO databases), and formula generator tools leveraging exact mass and isotopic pattern fidelity. Raw data are stored in open-format .d directories compliant with mzML 1.1.0 standards, ensuring interoperability with third-party platforms such as Skyline, XCMS Online, and OpenMS. All processing steps—including calibration application, background subtraction, and peak integration—are logged with timestamped metadata, supporting full traceability in quality-controlled laboratories.

Applications

• Non-targeted environmental analysis: Identification of emerging contaminants (e.g., PFAS precursors, halogenated flame retardants) in water, soil, and biota extracts
• Food safety screening: Detection of pesticide residues, mycotoxins, and adulterants at sub-ppb levels with confirmatory HRMS spectra
• Pharmaceutical impurity profiling: Structural characterization of low-abundance genotoxic impurities per ICH M7 guidelines
• Forensic toxicology: Differentiation of isomeric drugs of abuse (e.g., synthetic cannabinoids, fentanyl analogues) via accurate mass and retention index correlation
• Metabolomics: Retrospective analysis of archived GC-MS datasets using reprocessed FT spectra for novel biomarker discovery

FAQ

Does the Cassitrap 120K support tandem MS (MS/MS) fragmentation?
No—this system does not incorporate collision-induced dissociation (CID) or higher-energy C-trap dissociation (HCD) capabilities. Fragmentation is limited to electron ionization (EI) source conditions.
Can the FT-electrostatic trap mode operate simultaneously with quadrupole scanning?
No—acquisition modes are mutually exclusive and require manual or method-triggered switching; concurrent operation is not supported due to fundamental differences in ion duty cycle and detection electronics.
Is external calibration required for high-mass-accuracy measurements?
Internal calibration using co-eluting lock-mass compounds (e.g., perfluorotributylamine, PFTBA) is recommended for optimal long-term stability; external calibration is possible but not necessary for routine compliance-grade work.
What GC inlet types are supported?
Split/splitless, programmed temperature vaporizing (PTV), and cold-on-column inlets are fully supported via standardized API interfaces and pressure-controlled carrier gas delivery.
How is data integrity ensured during extended overnight runs?
The system implements cyclic checksum verification of raw transient data blocks, automatic recovery from transient communication loss, and write-locked .d directory structures to prevent accidental overwrites or partial saves.