Analytik Jena P25/25T Electrophoresis Power Supply
| Brand | Analytik Jena |
|---|---|
| Origin | Germany |
| Model | P25/25T |
| Output Voltage Range | 0–400 V |
| Output Current Range | 0–1000 mA |
| Operating Modes | Constant Voltage (CV), Constant Current (CC), Auto-Mode Switching |
| Memory Function | Last-Used Parameter Retention (non-volatile) |
| Timer Range | 1 min – 99 hr 59 min |
| Output Terminals | 4 independent dual-terminal pairs (8 total banana jacks) |
| Compliance | CE, IEC 61010-1 |
Overview
The Analytik Jena P25/25T Electrophoresis Power Supply is a laboratory-grade DC power source engineered for precision and reliability in nucleic acid and protein separation workflows. Designed according to the fundamental principles of electrophoretic migration—where charged biomolecules migrate under an applied electric field—the P25/25T delivers stable, low-noise DC output across a broad operational envelope. Its architecture supports both constant voltage (CV) and constant current (CC) regulation, with real-time auto-switching between modes based on load impedance changes—a critical feature during extended runs where gel resistance evolves due to buffer depletion or Joule heating. This dynamic response ensures consistent electric field strength and minimizes band distortion, particularly in high-resolution applications such as SDS-PAGE, native PAGE, agarose gel electrophoresis, and electroblotting. Built and tested in Jena, Germany, the unit complies with IEC 61010-1 for electrical safety in laboratory equipment and carries CE marking for conformity with European health, safety, and environmental protection standards.
Key Features
- Wide output range: 0–400 V DC and 0–1000 mA DC, enabling compatibility with mini-gels, large-format vertical systems, and high-throughput horizontal setups.
- Intelligent mode management: Simultaneous CV and CC operation with automatic transition—no manual reconfiguration required when resistance shifts during run progression.
- Non-volatile parameter memory: All user-defined settings—including voltage, current, timer duration, and mode selection—are retained after power cycling, eliminating repetitive setup for routine protocols.
- Precise digital timer: Adjustable from 1 minute to 99 hours and 59 minutes, with audible and visual end-of-run indication; prevents over-migration and gel exhaustion.
- Multi-system control capability: Four independent dual-terminal output channels (eight banana jacks total), each galvanically isolated, support concurrent operation of up to four electrophoresis or transfer units without cross-talk or load interference.
- Robust thermal design: Active cooling with temperature-monitored fan and overheat shutdown safeguards sustained performance during overnight transfers or high-power vertical runs.
Sample Compatibility & Compliance
The P25/25T is validated for use with standard electrophoresis formats including slab-gel (vertical and horizontal), capillary electrophoresis auxiliary supplies, semi-dry and wet-tank western blot transfer systems, and pulse-field electrophoresis accessories requiring stable DC bias. It meets the electrical safety requirements of IEC 61010-1:2010 and conforms to EN 61326-1 for electromagnetic compatibility (EMC) in laboratory environments. While not a regulated medical device, its stable output characteristics align with GLP documentation practices for method validation, and its non-volatile memory supports audit-ready parameter traceability—essential for laboratories operating under ISO/IEC 17025 or FDA-regulated quality systems.
Software & Data Management
The P25/25T operates as a standalone instrument with no proprietary software dependency. All configuration and monitoring occur via front-panel membrane keypad and high-contrast LCD display (dual-parameter readout: V/mA). Parameter logging is performed manually by users per experimental record; however, the persistent memory function enables reproducible setup across shifts and operators—supporting SOP adherence in QC/QA labs. For integration into LIMS or ELN ecosystems, optional analog output signals (0–5 V proportional to V/I) can be interfaced with external data acquisition modules compliant with IEEE 1451.2 transducer standards.
Applications
- SDS-PAGE and native PAGE for protein molecular weight estimation and conformational analysis
- Agarose and polyacrylamide gel electrophoresis of DNA fragments (0.1–20 kb)
- Semi-dry and tank-based electrotransfer of proteins to PVDF or nitrocellulose membranes
- Electroelution and electrofocusing pre-electrophoresis steps
- Charging of electrophoretic mobility shift assay (EMSA) gels and zymogram substrates
- Support for multiplexed lab workflows where parallel electrophoresis units require synchronized, independent power control
FAQ
Does the P25/25T support constant power (CP) mode?
No. The unit provides only constant voltage and constant current regulation, with automatic switching between them based on real-time load assessment.
Can the timer be paused or reset mid-run?
No. The timer is non-interruptible once initiated; it must complete or be manually stopped and restarted.
Are the output terminals isolated from earth ground?
Yes. Each of the four output channels features galvanic isolation and floating outputs, permitting flexible series/parallel configurations and eliminating ground-loop artifacts in multi-unit setups.
Is firmware upgrade capability available?
No. The P25/25T uses fixed-function embedded logic with no field-upgradable firmware; all functionality is hardware-defined at manufacture.
What type of fuses are used, and are spares included?
The unit employs standardized 1.6 A slow-blow ceramic fuses (5 × 20 mm); two spare fuses are supplied in the accessory compartment beneath the rear panel.
