CZGY SHY-2 Water-Bath Constant-Temperature Orbital Shaker

Overview

The CZGY SHY-2 Water-Bath Constant-Temperature Orbital Shaker integrates precise thermal regulation with controlled orbital agitation in a single compact unit. Engineered for reproducible incubation of biological and biochemical samples under defined hydrodynamic and thermal conditions, it operates on the principle of orbital shaking—where the platform rotates in a horizontal circular motion at adjustable speeds—while maintaining uniform water-bath temperature across the entire sample zone. This configuration minimizes shear stress compared to reciprocating or linear shakers, making it particularly suitable for sensitive cell cultures, microbial growth studies, solubility assays, and enzyme kinetics where consistent mixing and stable thermal environment are critical. The unit features a fully enclosed stainless steel water chamber with integrated heating elements and PID-controlled digital thermostatic regulation, ensuring rapid thermal equilibration and minimal drift over extended operation periods.

Key Features

• Digital LED display for simultaneous real-time monitoring of setpoint and actual temperature, as well as rotational speed (0–300 rpm)
• Continuously variable speed control via precision potentiometer—enabling fine-tuned optimization of mixing intensity without mechanical gear shifts
• Stainless steel (AISI 304) inner bath chamber and platform—resistant to corrosion from aqueous buffers, saline solutions, and mild organic solvents
• Universal spring-loaded clamp system—accommodates a wide range of vessel geometries including test tubes (10–50 mL), Erlenmeyer flasks (50–1000 mL), and multi-well plates with optional adapters
• Orbital motion profile (20 mm amplitude) generates gentle, laminar fluid motion—reducing foam formation and preventing splashing while ensuring homogeneous suspension of particulates and cells
• Programmable timer (0–120 minutes) with auto-shutdown functionality—supports unattended overnight protocols and standardized assay workflows
• Robust aluminum-alloy housing with insulated walls and splash-resistant control panel—designed for long-term reliability in shared laboratory environments

Sample Compatibility & Compliance

The SHY-2 is routinely deployed in applications requiring adherence to standard laboratory practices outlined in ISO/IEC 17025, CLSI guidelines, and internal GLP procedures. Its temperature uniformity (±0.5 °C) and stability (±0.5 °C) meet typical requirements for microbiological culture incubation per ISO 8664 and ASTM D4457. The stainless steel construction complies with FDA-recommended material specifications for equipment used in pharmaceutical process development. While not certified for IEC 61000-6-3 EMC compliance or UL/CSA safety listing out-of-the-box, the instrument incorporates grounded chassis design, over-temperature cutoff protection, and dry-run prevention circuitry—aligning with general electrical safety expectations for Class II laboratory appliances operating under 220 V AC supply.

Software & Data Management

The SHY-2 operates as a standalone analog-digital hybrid instrument with no embedded microprocessor or network interface. All operational parameters—including temperature setpoint, speed, and timer duration—are configured manually via front-panel controls. No proprietary software, drivers, or data logging capability is included. However, the device is compatible with external USB-connected thermocouple-based dataloggers (e.g., Omega OM-DAQPRO-5300 series) when paired with appropriate calibration probes placed directly in the water bath. For laboratories implementing 21 CFR Part 11-compliant workflows, manual entry of run parameters into electronic lab notebooks (ELNs) such as LabArchives or Benchling is recommended, supported by timestamped photographic documentation of the digital display prior to initiation.

Applications

• Microbial cultivation (E. coli, yeast, Bacillus spp.) in liquid media under aerobic conditions
• Cell suspension culture of mammalian and insect cell lines during expansion and transfection preparation
• Protein solubilization and refolding assays requiring simultaneous thermal denaturation and gentle agitation
• Extraction efficiency studies for plant metabolites, antibiotics, or environmental contaminants using aqueous or buffered solvent systems
• Standardization of dissolution testing for solid dosage forms in pharmacopeial method development (USP <711>)
• Preparation of uniform colloidal suspensions for nanoparticle synthesis and characterization workflows
• Hybridization and washing steps in molecular biology protocols involving membrane-based blotting techniques

FAQ

What is the maximum load capacity of the SHY-2 platform?
The platform supports up to 15 kg distributed evenly across its 40 × 31 cm surface area. Load imbalance exceeding ±10% should be avoided to maintain bearing longevity and oscillation consistency.
Can the unit operate continuously beyond 120 minutes?
Yes—the timer is bypassable via the “ON” position; continuous operation is permitted provided ambient ventilation meets minimum clearance requirements (≥10 cm on all sides) and water level is monitored regularly.
Is deionized water required for operation?
Distilled or deionized water is strongly recommended to minimize scale accumulation on heating elements and extend service intervals. Tap water may be used only if hardness is below 50 ppm and periodic descaling with 5% acetic acid is performed every 200 operating hours.
Does the SHY-2 support external temperature probe input?
No—it relies solely on built-in PT100 sensor feedback located near the center of the bath chamber. External probe integration requires third-party PID controllers with analog output compatibility.
What maintenance schedule is recommended?
Bi-weekly visual inspection of water level and heater integrity; quarterly cleaning of chamber interior with non-abrasive detergent; annual verification of temperature accuracy using NIST-traceable reference thermometer.