HengAo HAS-6A Six-Stage Air Microbial Sampler
| Brand | HengAo |
|---|---|
| Origin | Tianjin, China |
| Manufacturer | HengAo Technology Co., Ltd. |
| Model | HAS-6A |
| Sampling Flow Rate | 30 L/min |
| Flow Measurement Accuracy | ±1% FS |
| Timing Range | 0–99 min |
| Operating Temperature | −30 to +40 °C |
| Operating Relative Humidity | 20–80% RH |
| Weight | 6.5 kg |
| Dimensions (W×D×H) | 240 × 238 × 360 mm |
Overview
The HengAo HAS-6A Six-Stage Air Microbial Sampler is an engineered aerosol collection instrument designed for quantitative assessment of viable airborne microorganisms—including bacteria, fungal spores, and other culturable bioaerosols—across six discrete aerodynamic size fractions. It operates on the principle of inertial impaction, leveraging calibrated jet orifices and stage-specific collection surfaces to replicate the regional deposition patterns observed in the human respiratory tract (nasopharyngeal, tracheobronchial, and alveolar zones). This physiologically relevant fractionation enables researchers and environmental health professionals to evaluate not only total microbial load but also particle-size-resolved viability—a critical parameter for exposure risk assessment, HVAC system validation, cleanroom monitoring, and infection control studies in healthcare and pharmaceutical facilities.
Key Features
- Six-stage cascade impactor architecture with stainless-steel collection stages and standard Petri dish compatibility (90 mm diameter) for direct culture-based enumeration.
- Precise volumetric flow control at 30 L/min (±1% full-scale accuracy), maintained via integrated mass flow sensor feedback and brushless DC motor-driven vacuum system.
- Programmable sampling duration from 0 to 99 minutes with automatic shutdown and audible end-of-cycle alert.
- Delay-start function allows user-defined initiation time post-power-on, supporting unattended operation during off-hours or synchronized multi-point sampling campaigns.
- Robust mechanical design optimized for field deployment: compact footprint (240 × 238 × 360 mm), lightweight aluminum alloy housing, and operational stability across extreme ambient conditions (−30 to +40 °C; 20–80% RH).
- No internal filters or consumables required beyond standard agar plates—minimizing operational cost and cross-contamination risk between samples.
Sample Compatibility & Compliance
The HAS-6A supports standard microbiological media including Tryptic Soy Agar (TSA), Sabouraud Dextrose Agar (SDA), and malt extract agar (MEA), enabling simultaneous recovery of mesophilic bacteria and thermotolerant or xerophilic fungi. Each stage collects particles within defined aerodynamic diameter ranges (Stage 1: >7.0 µm; Stage 2: 4.7–7.0 µm; Stage 3: 3.3–4.7 µm; Stage 4: 2.1–3.3 µm; Stage 5: 1.1–2.1 µm; Stage 6: 0.65–1.1 µm), consistent with ISO 14698-1:2003 guidance on biocontamination control and aligned with USP recommendations for environmental monitoring in aseptic processing. Data generated meets GLP documentation requirements when paired with validated laboratory procedures and chain-of-custody records.
Software & Data Management
While the HAS-6A operates as a standalone hardware platform without embedded software, its analog timing interface and stable flow performance ensure compatibility with external data loggers and laboratory information management systems (LIMS). Users may integrate sampling metadata—including date/time stamps, location identifiers, operator ID, and environmental parameters—into audit-trail-compliant workflows per FDA 21 CFR Part 11 when coupled with electronic lab notebooks (ELNs) or validated spreadsheet templates. Calibration certificates traceable to national standards (e.g., CNAS-accredited labs in China) are provided upon request for regulatory submissions.
Applications
- Environmental monitoring of Grade A–D cleanrooms per ISO 14644 and EU GMP Annex 1.
- Assessment of bioaerosol dispersion dynamics in hospitals, laboratories, and animal facilities.
- Evaluation of air filtration efficiency and HVAC system performance under real-world loading conditions.
- Baseline characterization and routine surveillance of indoor air quality (IAQ) in educational, commercial, and residential buildings.
- Supporting outbreak investigations by correlating airborne microbial profiles with clinical isolates.
- Method development and validation for alternative rapid detection platforms (e.g., qPCR, immunoassays) requiring size-fractionated reference samples.
FAQ
What is the recommended maintenance interval for the HAS-6A?
Routine cleaning of impactor stages and nozzle assemblies with isopropyl alcohol is advised after each use; full calibration verification is recommended annually or after 200 operational hours.
Can the HAS-6A be used for non-viable particle sampling?
No—the HAS-6A is specifically configured for viable microorganism collection onto solid growth media; it does not support filter-based gravimetric or optical counting methods.
Is the flow rate adjustable beyond 30 L/min?
The instrument is factory-calibrated and certified for fixed-flow operation at 30 L/min; modification is not supported and would invalidate metrological traceability.
Does the device comply with international standards for bioaerosol sampling?
Yes—its cascade impactor geometry and performance characteristics align with ISO 14698-1, ASTM D6077, and EN 13098 for standardized environmental microbiological monitoring protocols.
How is sample integrity preserved during transport to the lab?
After sampling, Petri dishes should be sealed with parafilm, stored inverted at 4–8 °C, and processed within 4 hours for bacterial assays or within 24 hours for fungal analysis to maintain colony-forming unit (CFU) viability and minimize overgrowth artifacts.
