HengAo HSE-08C Semi-Automatic Multi-Channel Solid Phase Extraction System

Overview

The HengAo HSE-08C is a semi-automatic, multi-channel solid phase extraction (SPE) system engineered for reproducible, scalable sample preparation in analytical laboratories. It operates on the principle of selective adsorption and elution using disposable SPE cartridges—typically packed with silica-based or polymeric sorbents—to isolate target analytes from complex liquid matrices such as environmental water, biological fluids, food extracts, and pharmaceutical formulations. Unlike fully automated platforms requiring integrated robotics or high-pressure solvent delivery, the HSE-08C employs gravity-assisted and vacuum-assisted flow control with precision-adjustable peristaltic or pressure-regulated manifold systems, enabling consistent flow rates across all eight parallel channels. Its modular architecture supports method development flexibility while maintaining compatibility with widely adopted SPE protocols defined in ASTM D7977, ISO 16703, and EPA Method 502.2, making it suitable for routine QC labs, environmental testing facilities, and academic research groups performing LC-MS/MS or GC-MS sample cleanup prior to instrumental analysis.

Key Features

• Eight independent SPE channels with synchronized vacuum manifold control for parallel processing of up to eight samples per run
• Adjustable flow rate range from 1 to 60 mL/min—optimized for both slow conditioning/elution steps and rapid solvent exchange
• Five dedicated solvent ports supporting sequential or discrete application of loading, washing, and elution solvents without manual reservoir switching
• PTFE-wetted fluid path ensures chemical inertness and resistance to aggressive organic solvents (e.g., dichloromethane, acetonitrile, methanol) and acidic/basic aqueous media
• Modular cartridge holder accommodates standard 1 mL, 3 mL, and 6 mL SPE cartridges from major suppliers (e.g., Waters, Agilent, Thermo Fisher)
• Integrated vacuum gauge and adjustable vacuum regulator enable real-time monitoring and fine-tuning of suction pressure
• Compact benchtop footprint (W × D × H ≈ 420 × 320 × 380 mm) designed for space-constrained laboratory environments

Sample Compatibility & Compliance

The HSE-08C is validated for use with aqueous, alcoholic, and moderately organic liquid samples—including wastewater, plasma, urine, milk extracts, and plant tissue homogenates—provided they are pre-filtered to remove particulates >5 µm. Cartridge selection determines analyte recovery efficiency; common configurations include C18 for non-polar compounds, SCX for basic pharmaceuticals, and mixed-mode sorbents for ionizable analytes. The system complies with GLP documentation requirements when paired with laboratory-controlled SOPs and operator logbooks. While not inherently 21 CFR Part 11 compliant (as it lacks electronic audit trails or user authentication), its operational parameters can be recorded manually or interfaced via external data loggers for regulated environments under FDA or EMA-aligned quality systems.

Software & Data Management

The HSE-08C operates without embedded firmware or proprietary software—functioning as a hardware-only platform to maximize interoperability and reduce validation overhead. All method parameters (flow rate, solvent sequence, dwell time per step) are configured manually via calibrated knobs and labeled solvent lines. This design eliminates software licensing costs, cybersecurity vulnerabilities, and version-control dependencies. Laboratories implementing LIMS integration may document SPE runs using standardized templates aligned with ISO/IEC 17025 clause 7.5.2 (technical records), and traceability is maintained through batch-specific run logs referencing cartridge lot numbers, solvent batches, and analyst identifiers.

Applications

• Environmental analysis: Extraction of PAHs, PCBs, pesticides, and endocrine disruptors from surface water and wastewater
• Clinical toxicology: Isolation of opioids, benzodiazepines, and stimulants from human plasma and urine prior to LC-MS/MS quantification
• Food safety testing: Cleanup of mycotoxins (e.g., aflatoxin B1) and veterinary drug residues (e.g., sulfonamides, tetracyclines) from milk, honey, and meat extracts
• Pharmaceutical R&D: Desalting and buffer exchange of peptide synthesis crude products prior to HPLC purification
• Forensic chemistry: Concentration and matrix removal for hair or oral fluid screening workflows

FAQ

Is the HSE-08C compatible with 12-mL SPE cartridges?
Yes—its universal cartridge holder accepts standard footprints ranging from 1 mL to 12 mL, provided mechanical stability is ensured during vacuum application.
Can I use corrosive solvents like concentrated hydrochloric acid?
No—while PTFE offers broad chemical resistance, prolonged exposure to hot, concentrated mineral acids may compromise seal integrity; consult material compatibility charts before deployment.
Does the system support positive-pressure elution?
Not natively—the HSE-08C relies on vacuum-driven flow; however, external nitrogen manifolds can be coupled via optional PTFE tubing adapters for pressurized elution.
What maintenance is required?
Routine cleaning of the vacuum manifold and solvent lines with methanol followed by air-drying; annual inspection of PTFE gaskets and vacuum hose elasticity is recommended.
Is technical support available outside China?
Yes—HengAo provides remote troubleshooting, English-language operation manuals, and application notes via authorized international distributors; on-site service depends on regional partner coverage.