HengAo HBI-01 Constant-Temperature Aseptic Plate Dispensing System

Overview

The HengAo HBI-01 Constant-Temperature Aseptic Plate Dispensing System is an integrated laboratory automation platform engineered for precise, repeatable, and contamination-controlled liquid handling in life science workflows. Designed specifically for microbiology, cell culture, plant tissue culture, and pharmaceutical process support, the system combines thermostatic fluid conditioning with sterile plate dispensing in a single compact architecture. Its core operational principle relies on closed-loop temperature regulation of liquid media—whether molten agar, pre-warmed growth supplements, or chilled reagents—followed by volumetric delivery into standard Petri dishes, multiwell plates (6–96-well), or custom containers under laminar airflow-compatible conditions. Unlike ambient-temperature dispensers, the HBI-01 maintains fluid viscosity stability during transfer by actively managing thermal history: it supports rapid melt-and-hold of solidified media (e.g., agar-based formulations), controlled cooling of heat-sensitive solutions post-sterilization, and accelerated warming of refrigerated stocks—all while preserving sterility through localized surface decontamination and enclosed dispensing pathways.

Key Features

• Intuitive 7-inch capacitive touchscreen interface with programmable temperature profiles, dispensing volume presets, and real-time status monitoring
• Dual-zone thermal management: independent heating/cooling module for fluid reservoir (range: 4 °C to 95 °C, ±0.3 °C stability) and insulated dispensing chamber (ambient to 45 °C, ±0.5 °C)
• Integrated aseptic maintenance: UV-C irradiation + hot-air sterilization cycle for handheld dispensing gun (validated for ≥3-log reduction of common environmental microbes)
• Mechanically synchronized agitation: orbital shaking (0–300 rpm) and magnetic stirring (0–1,200 rpm) options ensure homogeneous suspension of particulates or viscous components prior to dispensing
• Flexible dispensing modes: continuous flow, single-shot, and multi-step batch dispensing with adjustable pause intervals between wells or plates
• Four-speed plate rotation control (0, 30, 90, 180 rpm) for uniform distribution of viscous or sediment-prone media across dish surfaces
• Active air-cooling system with adjustable fan speed for rapid thermal equilibration of heat-labile samples post-dispensing
• Footswitch-compatible I/O port enabling hands-free start/stop operation during glove-box or biosafety cabinet integration

Sample Compatibility & Compliance

The HBI-01 accommodates aqueous and semi-viscous biological fluids including nutrient broths, agarose gels (0.5–2.0% w/v), antibiotic-supplemented media, enzyme solutions, and cell suspension buffers. It is compatible with standard polystyrene, polypropylene, and glass-bottom Petri dishes (diameters 35–150 mm), as well as ANSI/SLAS-compliant microplates. The system adheres to ISO 13485 design controls for medical device manufacturing environments and supports GLP-compliant documentation via optional audit trail logging (timestamped user actions, parameter changes, and error events). While not certified for GMP production, its sealed fluid path, traceable calibration records, and configurable access levels meet foundational requirements for QC laboratories operating under FDA 21 CFR Part 11–aligned data integrity frameworks.

Software & Data Management

The embedded firmware supports protocol storage for up to 100 user-defined methods, each containing temperature ramp rates, dwell times, dispensing volumes per target, agitation sequences, and rotation profiles. All methods are exportable via USB 2.0 as CSV or XML files for version control and cross-instrument replication. Optional PC-based software (HengAo LabLink v3.2) enables remote monitoring, method synchronization across multiple HBI-01 units, and automated report generation compliant with ISO/IEC 17025 record retention guidelines. Audit trails include operator ID, timestamp, parameter values, and exception flags—retained for ≥12 months on internal flash memory.

Applications

• Preparation of agar plates for microbial isolation, antibiotic susceptibility testing (AST), and colony-forming unit (CFU) enumeration
• High-throughput seeding of mammalian or insect cell lines into multiwell formats under temperature-stabilized conditions
• Dispensing of thermosensitive plant tissue culture media containing phytohormones or antibiotics
• Pharmaceutical QC workflows requiring consistent volume delivery of sterile diluents or reference standards
• Biobanking operations where uniform cryoprotectant distribution into freezing vials is required prior to controlled-rate freezing

FAQ

What is the maximum viscosity the HBI-01 can dispense reliably?
The system is validated for fluids up to 500 mPa·s at 25 °C (e.g., 1.5% low-gelling-point agarose in PBS); higher viscosities require pre-heating and reduced dispensing speed.
Does the HBI-01 support serial dilution protocols?
Yes—via programmable multi-step dispensing with variable volume steps and inter-step mixing; manual pipette tip exchange is required between concentrations.
Can the system be integrated into a Class II biosafety cabinet?
Yes—the footprint (W×D×H: 420 × 380 × 360 mm) and rear-access service panel allow full enclosure; footswitch and USB connections route externally via pass-through ports.
Is calibration traceable to NIST or equivalent national standards?
Temperature sensors are factory-calibrated against PT100 reference probes traceable to CNAS-accredited labs; volumetric accuracy is verified using gravimetric ISO 8655-compliant procedures.
What maintenance intervals are recommended?
Daily wipe-down of dispensing surfaces; quarterly verification of thermal uniformity and dispensing repeatability; annual recalibration of temperature and volume subsystems by authorized service personnel.