DRETOP WYC-110X30R Low-Temperature Water Bath Orbital Shaker

Overview

The DRETOP WYC-110X30R is a precision-engineered low-temperature water bath orbital shaker designed for controlled incubation and agitation of biological and biochemical samples under thermally stable conditions. It integrates a thermostatically regulated water bath with a mechanically driven reciprocating shaking platform, enabling simultaneous temperature control and uniform mechanical stimulation—critical for microbial growth, cell suspension culture, enzyme kinetics, solubility studies, and extraction protocols. Unlike air-jacketed incubators or dry-block shakers, this unit leverages water’s high thermal mass and conductivity to achieve superior temperature homogeneity (±0.2 °C at 25 °C) across the entire bath volume. Its operational range extends from refrigerated 4 °C—enabling cold-sensitive protein handling or psychrophilic organism cultivation—to 100 °C for high-temperature sterilization cycles or accelerated dissolution testing. The system operates on a robust brushless DC motor architecture, eliminating carbon brush wear and ensuring consistent torque delivery across the full 0–220 rpm speed range.

Key Features

• High-fidelity temperature regulation via Pt100 resistance thermometer feedback and adaptive PID algorithm, delivering ±0.1 °C setpoint accuracy and <0.2 °C spatial uniformity at reference conditions;
• Reciprocating oscillation mode with fixed 35 mm amplitude and continuously adjustable frequency (0–220 rpm), optimized for suspension homogenization without foaming or shear-induced cell lysis;
• Electro-polished 304 stainless steel water bath and hinged lid—resistant to corrosion, microbial adhesion, and chemical degradation; facilitates rapid decontamination and compliance with GLP cleaning validation requirements;
• LED-backlit LCD interface displaying real-time temperature, speed, and remaining timer duration; supports parameter locking, offset calibration, and user-defined PID tuning for method transfer across laboratories;
• Power-loss memory function retains all active settings during unexpected outages, resuming operation automatically upon power restoration—ensuring protocol integrity in unattended overnight runs;
• Spring-clamp platform (435 × 260 mm) accommodates standard glassware including 250 mL Erlenmeyer flasks (11 units), test tubes, centrifuge tubes, and multi-well plates via adaptable retention geometry.

Sample Compatibility & Compliance

The WYC-110X30R supports aqueous and buffered liquid-phase samples compatible with stainless steel and borosilicate glass containment. Its 4–100 °C operating envelope meets ASTM E2912–22 (Standard Practice for Calibration of Laboratory Water Baths) and ISO 8570:2017 (Laboratory Equipment — Water Baths — Performance Requirements). The sealed bath design prevents evaporation-related concentration drift during extended assays (>24 h), while the absence of exposed heating elements minimizes thermal gradient artifacts. For regulated environments, the device supports audit-ready documentation workflows when paired with external data loggers compliant with FDA 21 CFR Part 11 (electronic records and signatures), particularly when used in QC microbiology (USP , ) or stability-indicating dissolution (USP ).

Software & Data Management

This model operates as a standalone instrument without embedded PC connectivity; however, its analog-compatible output signals (0–5 V or 4–20 mA optional via OEM module) enable integration into centralized laboratory monitoring systems (e.g., LabVantage, Siemens Desigo, or custom SCADA). All displayed parameters—including actual bath temperature, setpoint deviation, and elapsed time—are accessible via front-panel interface for manual SOP adherence. For traceable recordkeeping, users are advised to pair the unit with validated external thermistors and digital timers meeting ISO/IEC 17025 calibration intervals. Firmware does not store historical data internally, aligning with GMP Annex 11 principles that prioritize deterministic, non-volatile control logic over proprietary software dependencies.

Applications

• Microbial culture expansion (E. coli, yeast, bacilli) under defined thermal and agitation conditions per CLSI M07-A10;
• Suspension-based mammalian cell passaging where gentle mixing prevents sedimentation without inducing shear stress;
• Protein solubilization and refolding assays requiring precise sub-ambient temperatures (4–15 °C) to suppress aggregation;
• Environmental sample extraction (e.g., PAHs, PCBs) per EPA Method 3510C using aqueous/organic biphasic systems;
• Pharmaceutical dissolution testing of immediate-release tablets under USP Apparatus I (basket) or II (paddle) auxiliary agitation support;
• Antibiotic susceptibility testing (AST) involving broth microdilution with continuous shaking to maintain planktonic growth phase.

FAQ

Does the WYC-110X30R support both reciprocating and orbital motion modes?

No—this model is configured exclusively for reciprocating (linear back-and-forth) oscillation. Orbital motion is available in other DRETOP series (e.g., WYC-110X50R with dual-mode option), but not on the X30R variant.
Is the water bath depth sufficient for full immersion of 250 mL flasks?

Yes—the internal bath height of 250 mm allows complete submersion of standard 250 mL Erlenmeyer flasks (height ≈ 170 mm) with adequate headspace for thermal expansion and agitation clearance.
Can the unit be operated continuously for 72+ hours?

Yes—designed for unattended long-term operation, it includes over-temperature cutoff, short-circuit detection, and thermal runaway protection per IEC 61010-1 safety standards.
What maintenance is required for the brushless DC motor?

None—brushless construction eliminates scheduled commutator servicing; only periodic inspection of drive coupling integrity and bath water quality (deionized/distilled recommended) is advised.
Is calibration certificate included with shipment?

No—NIST-traceable calibration documentation is available as an optional add-on service and must be requested at time of order; factory verification reports cover temperature uniformity and timing accuracy per ISO/IEC 17025 guidelines.