PerkinElmer Series 200A Fluorescence Detector

Overview

The PerkinElmer Series 200A Fluorescence Detector is a high-sensitivity, benchtop-compatible HPLC detector engineered for quantitative and qualitative analysis of native or derivatized fluorescent compounds. Based on dual-monochromator optical architecture, it operates on the principle of excitation-emission wavelength selectivity—employing a xenon arc lamp source, adjustable excitation (200–750 nm) and emission (200–750 nm) monochromators, and a photomultiplier tube (PMT) detector with low-noise signal amplification. Designed for integration into legacy and modern LC systems—including PerkinElmer Series 200/400 HPLC platforms—the 200A delivers stable baseline performance, linear dynamic range exceeding 10⁴, and reproducible response across extended run times. Its mechanical and electronic subsystems are fully refurbished to OEM functional specifications, including lamp alignment verification, monochromator wavelength calibration, PMT gain optimization, and leak-tight flow cell validation.

Key Features

• Refurbished and functionally validated by SpectraLab Scientific’s certified instrumentation engineers using NIST-traceable standards
• Dual-grating monochromators enabling independent, precise selection of excitation and emission wavelengths with ≤1.5 nm bandwidth
• High-efficiency fused-silica flow cell (10 µL path length, 1 mm i.d.) compatible with standard 1/16″ stainless steel tubing and common HPLC mobile phases
• Analog output (0–1 V full scale) and TTL-compatible status signals for seamless integration with third-party data systems
• Front-panel controls with LED display for real-time monitoring of lamp status, wavelength settings, and signal intensity
• Robust thermal management ensuring <±0.5 °C temperature stability in the optical compartment during continuous operation
• No software dependency for basic operation—fully operational as a standalone analog detector without proprietary acquisition software

Sample Compatibility & Compliance

The 200A supports detection of analytes exhibiting intrinsic fluorescence (e.g., polycyclic aromatic hydrocarbons, amino acids, vitamins B2/B6, quinolones) or those rendered fluorescent via post-column derivatization (e.g., o-phthalaldehyde for primary amines, dansyl chloride for secondary amines). It complies with core instrumental requirements outlined in ASTM D5503 (fluorescence detection in environmental analysis), USP (analytical instrument qualification), and ICH Q2(R2) guidelines for detector performance verification. All refurbished units undergo IQ/OQ protocol execution per GLP-aligned procedures, including wavelength accuracy verification (±2 nm), signal-to-noise ratio assessment (>1000:1 at 254/365 nm), and linearity testing over five decades of concentration (R² ≥ 0.9998). Documentation includes Certificate of Refurbishment, Test Report, and Traceability Statement.

Software & Data Management

While the Series 200A operates independently of proprietary software, its analog voltage output is fully compatible with industry-standard chromatography data systems (CDS), including Empower™, Chromeleon™, OpenLAB CDS, and custom LabVIEW-based acquisition platforms. SpectraLab provides optional configuration support for time-based event triggering, peak area integration mapping, and baseline drift compensation routines. For audit-trail compliance in regulated environments, the detector’s hardware-level signal integrity ensures compatibility with FDA 21 CFR Part 11–enabled CDS deployments when paired with appropriate system-level electronic signatures and access controls. No embedded firmware updates or cloud connectivity are present—consistent with legacy detector design principles emphasizing deterministic, non-volatile operation.

Applications

• Pharmaceutical QC testing of fluorescent APIs and impurities per USP monographs (e.g., tetracyclines, fluoroquinolones)
• Environmental monitoring of PAHs in soil extracts and wastewater effluents (EPA Method 8310)
• Food safety analysis of mycotoxins (aflatoxin B1, ochratoxin A) following AOAC 999.05 derivatization protocols
• Life science research involving fluorescently labeled peptides, nucleotides, and metabolic intermediates
• Academic teaching labs requiring rugged, serviceable fluorescence detection for HPLC method development courses

FAQ

Is this unit covered under manufacturer warranty?
No—PerkinElmer’s original warranty has expired. SpectraLab Scientific provides a 6-month limited warranty covering defects in materials and workmanship related to refurbishment.
Does the detector include a flow cell and lamp?
Yes—each unit ships with a validated quartz flow cell and a new-per-specification xenon arc lamp installed and optically aligned.
Can it be integrated with non-PerkinElmer HPLC systems?
Yes—via standard analog input (0–1 V) and TTL trigger interfaces; mechanical mounting brackets and tubing adapters are included.
What documentation accompanies the instrument?
Certificate of Refurbishment, Full Test Report (wavelength accuracy, S/N, linearity), NIST-traceable calibration records, and operational manual.
Is remote technical support available after purchase?
Yes—SpectraLab offers email and phone-based application and integration support for 12 months post-delivery at no additional cost.