Illumina HiSeq 2000 Next-Generation Sequencing System
| Origin | USA |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | HiSeq 2000 |
| Warranty Period | 6 months |
| Instrument Age | 3–4 years |
| Instrument Brand | Illumina |
| Product Category | NGS Platform |
| Configuration Status | Fully Tested & Refurbished |
Overview
The Illumina HiSeq 2000 Next-Generation Sequencing System is a high-throughput, short-read DNA sequencing platform engineered for precision, scalability, and reproducibility in genomics research laboratories. Based on Illumina’s proven sequencing-by-synthesis (SBS) chemistry with reversible dye-terminators, the HiSeq 2000 utilizes patterned flow cell technology and cluster generation via bridge amplification to enable massively parallel sequencing of millions of DNA fragments per run. Designed for academic core facilities, clinical research labs, and contract research organizations (CROs), this system delivers up to 600 Gb of raw data per 14-day dual-flow-cell run—achieving throughput that was industry-leading at its time of deployment. Its architecture supports both single-read and paired-end sequencing modes, with read lengths configurable up to 101 cycles per end. The system integrates fluidics, optics, and thermal control subsystems into a robust, benchtop footprint optimized for stable long-term operation under GLP-aligned laboratory conditions.
Key Features
- Patterned flow cell technology enabling high-density cluster generation and improved signal-to-noise ratio
- Reversible terminator chemistry ensuring high base-calling accuracy (Q30 ≥ 85% typical)
- Dual-flow-cell configuration supporting concurrent or staggered runs for improved lab throughput
- Integrated cluster station and sequencer in a single instrument chassis with automated reagent handling
- On-instrument real-time image acquisition and base calling via Illumina Real-Time Analysis (RTA) software
- Refurbished and functionally validated by SpectraLab Scientific—including full optical alignment, fluidic integrity testing, and calibration verification
- Comprehensive post-purchase support including remote diagnostics and protocol optimization guidance
Sample Compatibility & Compliance
The HiSeq 2000 accepts standard Illumina-compatible library preparations, including those generated from genomic DNA, RNA (via cDNA conversion), ChIP-seq, methyl-seq, and targeted enrichment workflows. Libraries must be adapter-ligated using TruSeq or equivalent compatible kits and quantified prior to loading. The system complies with standard biosafety Level 2 (BSL-2) operational requirements and meets electromagnetic compatibility (EMC) standards per IEC 61326-1. While not FDA-cleared for diagnostic use, the platform is routinely employed in research-use-only (RUO) applications aligned with ISO/IEC 17025 quality management principles. All refurbished units undergo documentation review to ensure traceability of maintenance history and adherence to manufacturer-recommended service intervals.
Software & Data Management
The HiSeq Control Software (HCS) v2.2.x or later provides instrument control, run monitoring, and integrated base calling. Raw BCL files are converted to FASTQ format using CASAVA or bcl2fastq v1.8.4+, supporting demultiplexing and QC metrics generation. Data output conforms to standard NGS file formats (FASTQ, BAM, VCF) compatible with downstream analysis pipelines including GATK, Bowtie2, STAR, and DESeq2. Audit trails for run parameters, user logins, and software versioning are retained per internal lab SOPs. Although native support for 21 CFR Part 11 compliance is limited in legacy HCS versions, integration with third-party LIMS platforms enables electronic signature capture, role-based access control, and change history logging suitable for regulated environments operating under GLP or GCP frameworks.
Applications
- Whole-genome sequencing (WGS) of human, model organism, and microbial genomes
- RNA-Seq for transcriptome profiling, differential expression, and splice variant detection
- ChIP-Seq and ATAC-Seq for epigenomic mapping of protein–DNA interactions and chromatin accessibility
- Small RNA-Seq for miRNA and piRNA discovery and quantification
- Targeted resequencing panels (e.g., exome capture, oncology gene panels) using hybridization-based enrichment
- Metagenomic profiling of complex environmental or microbiome samples
FAQ
Is this HiSeq 2000 unit fully refurbished and tested before shipment?
Yes. Each system undergoes comprehensive functional validation including optical path calibration, fluidic leak testing, cluster density optimization, and a minimum two-cycle test run with PhiX control library.
What consumables and accessories are included with the instrument?
The system ships with one complete set of flow cells, reagent kits for cluster generation and sequencing (v4 chemistry), and all necessary hardware modules—including cBot (if applicable), HiScanSQ scanner, and associated cables and power supplies.
Does the 6-month warranty cover parts, labor, and on-site service?
The warranty includes parts replacement and remote technical support. On-site service is available as an optional add-on and subject to regional technician availability.
Can this instrument be integrated into a modern bioinformatics infrastructure?
Yes. Output files are fully compatible with current open-source and commercial NGS analysis suites. We provide documentation for integration with common HPC and cloud-based analysis environments.
Are original Illumina service records available for this unit?
Where available, full service logs—including previous firmware updates, major component replacements, and calibration reports—are provided upon request and included in the delivery package.
