Agilent 1290 Infinity II Liquid Chromatograph (Refurbished, Certified Pre-Owned)

Overview

The Agilent 1290 Infinity II Liquid Chromatograph is a certified pre-owned, factory-refurbished ultra-high-performance liquid chromatography (UHPLC) system engineered for maximum resolution, sensitivity, and method transfer fidelity in regulated and research laboratories. Built upon Agilent’s proprietary dual-piston binary pump architecture with active solvent compressibility compensation, the system delivers precise gradient delivery at pressures up to 1200 bar—enabling full compatibility with sub-2 µm particle columns such as ZORBAX RRHD 1.8 µm. Its core measurement principle relies on high-pressure liquid phase separation coupled with real-time UV-Vis spectral detection via microfluidic optical waveguide technology. The system is designed for seamless integration into existing LC and LC/MS workflows, supporting method portability across Agilent and third-party UHPLC/HPLC platforms without revalidation. All units undergo comprehensive functional testing—including pressure integrity, gradient accuracy, retention time reproducibility (<0.15% RSD, n=6), baseline noise (<±0.25 mAU), and autosampler precision (<0.25% RSD)—per Agilent’s PQ/QC specifications prior to release.

Key Features

• Binary pump with ≤10 µL delay volume and active damping—enables sub-second gradient response for LC/UV and LC/MS applications
• Carbon-silicon piston technology—enhances seal longevity and reduces maintenance frequency
• Integrated high-efficiency degasser with rapid solvent priming capability
• Agilent Max-Light cartridge flow cells—10 mm standard and 60 mm high-sensitivity variants deliver near-100% optical transmission and minimal band broadening
• Programmable slit width (1–8 nm) for dynamic optimization of signal-to-noise ratio, linearity, and spectral resolution
• Peltier-controlled column compartment with dual-zone temperature control (–10 °C to 100 °C, ±0.05 °C stability)
• Flexible Cube module—supports fixed-loop injection, needle seat backflush, and zero-dead-volume valve switching for multi-method development
• Autosampler with overlapping injection cycle, 4–40 °C programmable sample cooling, and inert sealing materials to minimize carryover (<0.005% typical)

Sample Compatibility & Compliance

The 1290 Infinity II accommodates standard and narrow-bore columns (1.0–4.6 mm ID), including 1.8 µm RRHD, sub-2 µm BEH, and porous graphitic carbon phases. It supports aqueous-organic mobile phases, ion-pairing reagents, and high-pH solvents within its chemically resistant fluidic path (titanium, PEEK, and sapphire components). All refurbished systems are verified against ASTM E2689 for HPLC system suitability and aligned with ISO/IEC 17025 technical requirements for calibration traceability. Configuration options include 21 CFR Part 11-compliant audit trails, electronic signatures, and secure user access levels when deployed with OpenLab CDS. Documentation packages include Certificate of Performance Verification, PQ/QC test reports, and full service history logs.

Software & Data Management

Control and data acquisition are managed through Agilent OpenLab CDS (version B.02.10 or later), a validated, scalable chromatography data system supporting GxP environments. The interface provides real-time instrument status monitoring, drag-and-drop method building, automated system suitability assessment, and customizable reporting templates. Raw data files (.D format) are stored with embedded metadata (flow cell serial number, lamp usage hours, column history via RFID tagging), ensuring full analytical traceability. Data export complies with ASTM E1921 and CDISC standards; integration with LIMS and ELN platforms is supported via ODBC and RESTful APIs. All systems ship with licensed software, USB security keys, and installation validation documentation.

Applications

This platform is routinely deployed in pharmaceutical QC (assay, impurity profiling, dissolution testing), biopharmaceutical characterization (mAb aggregation, glycan analysis), environmental contaminant screening (PAHs, pesticides), food safety (mycotoxins, additives), and academic metabolomics. Its high-pressure capability enables rapid method development using kinetic plots and column selectivity screening. The low dispersion design ensures compatibility with mass spectrometry interfaces—including Jet Stream and Dual AJS sources—without compromising peak capacity or sensitivity. Method transfer success rates exceed 95% between Agilent 1100/1200/1260/1290 platforms and selected non-Agilent UHPLC systems under controlled parameter mapping.

FAQ

Is this unit covered by Agilent’s original factory warranty?
No—this is a certified pre-owned instrument with a 6-month limited hardware warranty administered by the refurbishing provider, backed by full functional verification documentation.
Can I use non-Agilent columns and detectors with this system?
Yes—the 1290 Infinity II features industry-standard fittings, analog/digital I/O ports, and open communication protocols (LAN, RS-232) for interoperability with third-party columns, fraction collectors, and mass spectrometers.
What documentation accompanies the instrument?
Each unit ships with a Certificate of Performance Verification, PQ/QC test report, service history log, OpenLab CDS license certificate, and safety/compliance summary aligned with ISO/IEC 17025 practices.
Is method transfer support included?
Yes—technical documentation includes gradient translation worksheets, dwell volume characterization data, and column dead volume correction tables to facilitate cross-platform method migration.
Are consumables and spare parts available post-purchase?
All critical spares—including Max-Light flow cells, pump seals, autosampler rotors, and column oven heaters—are stocked and available with same-day shipping under standard catalog part numbers.