AB SCIEX API 3200 MD Triple Quadrupole Liquid Chromatography Tandem Mass Spectrometer (Used)

Overview

The AB SCIEX API 3200 MD is a high-performance, used triple quadrupole liquid chromatography–tandem mass spectrometer (LC-MS/MS) engineered for robust, regulatory-compliant quantitative analysis of small molecules in complex matrices. Built upon SCIEX’s proven ion optics architecture and LINAC™ collision cell design, the system delivers high sensitivity, excellent selectivity, and exceptional day-to-day reproducibility—critical attributes for laboratories operating under GLP, GMP, or clinical research frameworks. Its core measurement principle relies on electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI), followed by mass filtering in Q1, fragmentation via controlled collision-induced dissociation (CID) in the linear accelerator (LINAC) collision cell, and product ion detection in Q3. This targeted MS/MS workflow enables highly specific quantitation even in challenging biological, environmental, or food safety samples where matrix interferences are prevalent.

Key Features

• Turbo V™ ion source with integrated ceramic heating and optimized gas dynamics—enables stable ionization across LC flow rates from 5 µL/min to 3 mL/min, minimizes carryover, and supports rapid polarity switching between ESI+ and ESI−
• LINAC™ collision cell architecture—provides fast dwell times, high transmission efficiency, and consistent fragmentation kinetics without loss of sensitivity during multi-analyte scheduled MRM acquisition
• Curtain Gas™ interface—reduces neutral and solvent cluster contamination at the orifice, significantly extending source cleaning intervals and improving long-term signal stability
• Modular ion source options—DuoSpray™ (dual ESI/APCI probe), PhotoSpray™ (photoionization for non-polar compounds), and NanoSpray™ (low-flow/nano-LC compatibility) allow method flexibility without hardware reconfiguration
• Integrated Shimadzu Prominence LC-20A system—including LC-20AD binary pumps, SIL-20A HT autosampler (1.5 µL injection precision, <0.5% RSD), CTO-20A column oven (4–85 °C), and DGU-20A3 vacuum degasser—ensures chromatographic reliability and gradient reproducibility
• Analyst® software platform—supports automated method development, scheduled MRM optimization, calibration curve fitting (linear/log-linear with weighting), internal standard ratio calculation, and audit-trail-enabled data review per FDA 21 CFR Part 11 requirements

Sample Compatibility & Compliance

The API 3200 MD is routinely deployed in laboratories performing regulated quantitative bioanalysis (e.g., pharmacokinetics, toxicology), environmental contaminant screening (e.g., pesticides, PFAS), food adulterant testing (e.g., mycotoxins, veterinary drug residues), and clinical diagnostics (e.g., steroid profiling, vitamin D metabolites). It complies with analytical performance criteria outlined in USP , ICH M10, and ISO/IEC 17025:2017 for method validation. The instrument has been verified to meet typical acceptance criteria for carryover (<0.1%), intra-day and inter-day precision (<15% CV), and accuracy (85–115%) when operated with appropriate QC standards and IS normalization. All hardware components—including vacuum system, detector electronics, and RF power supplies—have undergone functional verification at SpectraLab Scientific prior to resale.

Software & Data Management

Data acquisition and processing are fully managed through SCIEX Analyst® software (v1.6.2 or higher), which provides a validated, traceable environment for method setup, instrument control, peak integration, and report generation. The software supports full electronic signature capability, user access level management, and secure audit trail logging for all critical actions—including parameter changes, calibration updates, and result approvals. Raw data files (.wiff) are stored in a structured, vendor-neutral format compatible with third-party reprocessing tools (e.g., Skyline, MultiQuant™). Instrument control firmware is up-to-date and compatible with Windows 10 (64-bit) operating systems. No legacy licensing restrictions apply; full software installation media and activation keys are included.

Applications

• Pharmaceutical bioanalysis: plasma, urine, and tissue extraction workflows for small-molecule drugs and metabolites
• Clinical toxicology: simultaneous quantification of opioids, benzodiazepines, stimulants, and antidepressants in human specimens
• Environmental monitoring: detection of endocrine disruptors, pharmaceuticals in wastewater, and persistent organic pollutants in soil extracts
• Food safety: multi-residue analysis of antibiotics, antifungals, and mycotoxins in milk, meat, and cereal matrices
• Academic research: structural elucidation support via product ion scanning and precursor ion mapping in natural product chemistry

FAQ

Is this unit fully refurbished and tested prior to shipment?
Yes. Each API 3200 MD undergoes full functional validation—including mass calibration, resolution verification, MRM sensitivity assessment (using caffeine and reserpine), source cleanliness inspection, and LC gradient integrity testing—by certified SCIEX-trained engineers at SpectraLab Scientific.
Does the system include original manufacturer documentation and service history?
All units ship with original SCIEX operation manuals (digital and printed), Shimadzu LC system documentation, and a detailed pre-shipment test report outlining performance metrics against factory specifications.
Can the system be configured for 21 CFR Part 11 compliance?
Yes. Analyst® software can be deployed in a validated, audit-trail-enabled configuration with role-based permissions, electronic signatures, and immutable data archiving—subject to customer-specific IT infrastructure validation.
What level of technical support is provided post-purchase?
SpectraLab offers 90 days of remote application and operational support, including method troubleshooting, software configuration assistance, and basic maintenance guidance. Extended service contracts and on-site engineering support are available upon request.
Are consumables and spare parts included or available separately?
Standard consumables (e.g., ESI needles, curtain gas filters, turbo pump oil) are not included but are stocked and shipped within 48 hours globally. Critical spares—including Turbo V source housings, QJet lenses, and detector assemblies—are available ex-stock for urgent replacement needs.