Agilent 6110A Single Quadrupole LC/MS System (Refurbished)

Overview

The Agilent 6110A Single Quadrupole LC/MS System is a robust, benchtop mass spectrometer engineered for reliable quantitative and qualitative analysis in regulated and research laboratories. Coupled with the Agilent 1200 Prep HPLC platform, this refurbished system delivers high reproducibility in liquid chromatography–mass spectrometry workflows. The 6110A operates on quadrupole mass filtering principles—utilizing radiofrequency (RF) and direct current (DC) voltages to selectively transmit ions of defined m/z ratios through a stable mass filter. Its design supports both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI), enabling broad compound coverage across polar, semi-polar, and moderately nonpolar analytes. The system is optimized for routine applications including impurity profiling, method development support, stability-indicating assays, and targeted quantitation in pharmaceutical, environmental, and academic settings.

Key Features

• Single quadrupole mass analyzer with mass range spanning 10–1500 m/z, suitable for small-molecule characterization and verification
• High scan speed of 2500 u/s in full-scan mode, facilitating rapid chromatographic peak detection and spectral fidelity
• Mass accuracy of ±0.13 u across the calibrated range in scan acquisition, supporting confident molecular weight assignment
• Long-term mass stability of ±0.1 u over 12 hours—critical for extended batch runs and unattended operation
• Dual ionization capability: ESI (400 µL/min flow rate compatible) and APCI (1000 µL/min), with automatic source switching and parameter recall
• Integrated Agilent 1200 Prep HPLC platform featuring dual G1361A preparative pumps, G2258A autosampler with 100-position tray capacity, G1364B fraction collector, and G1315B diode array detector
• Pre-validated refurbishment process performed by SpectraLab Scientific—including vacuum integrity verification, ion optic alignment, detector gain calibration, and system performance testing per Agilent-recommended protocols

Sample Compatibility & Compliance

The 6110A accommodates a wide range of sample types—from aqueous extracts and organic solvent-based injections to complex biological matrices (e.g., plasma, urine, tissue homogenates) when paired with appropriate sample preparation and LC separation. Its ESI interface supports reversed-phase, HILIC, and ion-pairing chromatography; APCI complements less polar compounds resistant to protonation. The system meets foundational requirements for GLP-compliant environments and supports audit-ready data handling when operated with compliant software configurations. While the 6110A itself does not natively enforce 21 CFR Part 11 controls, its data files (in .D format) are compatible with Agilent MassHunter Workstation (vB.07 or later), which may be configured for electronic signatures, audit trails, and user access control under validated laboratory IT infrastructure.

Software & Data Management

Data acquisition and processing are performed using Agilent MassHunter Qualitative and Quantitative Analysis software. Raw data are stored in vendor-native .D directories, preserving full spectral metadata, acquisition parameters, and instrument logs. The software enables centroided and profile-mode processing, extracted ion chromatogram (XIC) generation, retention time alignment, and response factor-based quantitation. All system configuration files, method templates, and calibration reports are retained post-refurbishment and delivered with the instrument. SpectraLab provides documented evidence of system suitability tests (SST), including reserpine sensitivity verification (10 pg on-column, m/z 609.3, 100:1 RMS S/N), mass calibration at multiple points, and baseline noise assessment—supporting immediate integration into existing QC/QA workflows.

Applications

• Pharmaceutical quality control: assay validation, degradation product identification, residual solvent analysis
• Academic metabolomics: targeted profiling of endogenous small molecules (e.g., amino acids, organic acids, nucleotides)
• Environmental monitoring: pesticide residue screening in food and water samples
• Natural product isolation support: real-time fraction tracking and compound-triggered collection via UV-MS correlation
• Method transfer and development: compatibility with USP , ICH Q2(R2), and ASTM D7260-19 guidelines for LC-MS system suitability

FAQ

Is this Agilent 6110A system fully refurbished and performance-verified?
Yes. Each unit undergoes comprehensive functional testing, including vacuum leak checks, ion transmission optimization, mass calibration, sensitivity validation (reserpine SIM), and HPLC gradient integrity verification prior to shipment.
What documentation is included with the system?
You receive a complete refurbishment report, system suitability test results, instrument configuration log, Agilent factory service history (where available), and a signed 6-month limited warranty certificate.
Can this system be integrated into an existing LIMS or ELN environment?
Raw .D files and processed reports can be exported in ASCII, CSV, or PDF formats. MassHunter supports ODBC connectivity for structured database ingestion, though custom middleware may be required for direct LIMS interfacing.
Does the system include original Agilent software licenses?
The system ships with legally licensed MassHunter software (Qualitative and Quantitative modules), activated under a perpetual license tied to the instrument’s serial number.
Is on-site installation and qualification support available?
SpectraLab offers optional IQ/OQ services performed by certified field engineers, including URS review, protocol execution, and final sign-off documentation aligned with ISO/IEC 17025 and FDA expectations.