TSP P4000 HPLC Pump and AS3000 Autosampler System
| Origin | Canada |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported Instrument |
| Model | P4000 / AS3000 |
| Operational Age | 4–5 Years |
| Warranty | 6 Months |
| Product Category | Chromatography Systems |
| Compliance | Fully Refurbished & Factory-Tested |
Overview
The TSP P4000 HPLC Pump and AS3000 Autosampler System is a fully refurbished, high-reliability liquid chromatography platform originally engineered by Thermo Separation Products (TSP), a predecessor brand of Thermo Fisher Scientific. This dual-component system integrates a precision reciprocating piston pump capable of delivering stable, pulse-free solvent delivery across a wide flow range (typically 0.001–10.0 mL/min), and a robust, temperature-controlled autosampler designed for reproducible injection volumes (1–100 µL) with low carryover (<0.01%). The system operates on standard HPLC principles—employing high-pressure mobile phase delivery, column-based analyte separation, and post-column detection compatibility—and is suitable for routine QC, method development, and stability-indicating assays in regulated and non-regulated laboratories.
Key Features
- Refurbished P4000 Binary or Quaternary Gradient Pump: Equipped with dual stainless-steel pump heads, active backpressure control, and integrated degasser compatibility; supports gradient elution with ≤0.2% RSD flow precision at 1 mL/min.
- AS3000 Autosampler: Features a 100-position vial tray (2-mL standard), programmable injection sequence, needle wash station with dual-solvent capability, and ambient or refrigerated sample compartment (4–40 °C option available).
- Full System Validation: Each unit undergoes comprehensive functional testing—including pressure leak verification, flow accuracy calibration, injection volume linearity assessment, and gradient composition verification per ASTM D7218 and ISO 17025-aligned protocols.
- Hardware Consistency: All fluidic pathways use bio-inert materials (e.g., PEEK, stainless steel 316); pump seals and autosampler rotor seals are replaced with OEM-spec components during refurbishment.
- Interfacing Flexibility: Standard RS-232 and analog voltage outputs enable seamless integration with legacy or modern data systems, including Chromeleon CDS, Empower, or custom LabVIEW-based acquisition platforms.
Sample Compatibility & Compliance
The P4000/AS3000 system accommodates a broad range of sample matrices—including aqueous buffers, organic solvents (acetonitrile, methanol), and mildly corrosive mobile phases—when operated within manufacturer-specified pH (1.5–12.5) and pressure (≤400 bar) limits. It complies with core instrumentation requirements outlined in USP , ICH Q2(R2), and FDA 21 CFR Part 11 when paired with compliant chromatography data systems (CDS) supporting audit trails, electronic signatures, and user access controls. As a refurbished instrument, it carries full traceability documentation: refurbishment log, test certificates, component replacement records, and pre-shipment performance report—all provided digitally upon purchase.
Software & Data Management
While the P4000 and AS3000 operate independently of proprietary software (no built-in controller), they are fully compatible with industry-standard CDS platforms. SpectraLab Scientific validates each system’s communication integrity with Chromeleon 7.2+ and Empower 3 SP2 or later, confirming reliable command execution (e.g., start/stop, valve switching, injection triggering) and status feedback (pressure, flow, injection confirmation). Raw analog output signals (e.g., 0–1 V for pressure, 0–5 V for flow) are calibrated and documented to support analog-to-digital conversion in custom DAQ environments. All refurbished units include firmware version logs and are verified against known stable microcode releases to prevent timing-related injection errors or pump stall events.
Applications
This system is routinely deployed in pharmaceutical QC labs for assay and impurity profiling of small-molecule APIs; in academic research for natural product fractionation and polymer molecular weight distribution analysis; and in environmental testing for pesticide residue quantification in soil/water extracts. Its mechanical durability and consistent dwell volume (≤800 µL for P4000 with standard mixer) make it especially suited for isocratic and shallow-gradient methods where retention time stability is critical. Users report >98% uptime over 12-month operational periods when maintained per TSP-recommended service intervals (seal replacement every 6 months, pump head polishing annually).
FAQ
Is this system compatible with modern detectors (e.g., UV-Vis, MS)?
Yes—the P4000 and AS3000 feature standard 1/16″ tubing ports and TTL/RS-232 trigger interfaces, enabling direct synchronization with third-party detectors including Agilent 1260 UV, Waters Xevo TQ-S, and Shimadzu SPD-M30A.
What documentation is included with purchase?
Each unit ships with a Certificate of Refurbishment, Functional Test Report (including flow accuracy, pressure hold, injection repeatability), OEM parts replacement list, and firmware revision history.
Can the system be configured for GMP/GLP compliance?
Yes—when connected to a 21 CFR Part 11-compliant CDS and operated under documented SOPs, the hardware meets baseline requirements for regulated environments; SpectraLab provides optional IQ/OQ protocol templates upon request.
Are spare parts and service support available?
All critical consumables (pump seals, autosampler rotors, needle seats) remain commercially available through Thermo Fisher and third-party OEM-qualified suppliers; SpectraLab offers remote troubleshooting and on-site service contracts globally.
What is the expected remaining service life after refurbishment?
Based on operational history and component wear analysis, units exhibit median residual service life of 6–8 years under typical lab usage (≤8 hrs/day, ≤5 days/week), assuming adherence to preventive maintenance schedules.
