PerkinElmer Lambda 40 UV-Vis Spectrophotometer (Refurbished)

Overview

The PerkinElmer Lambda 40 UV-Vis Spectrophotometer is a precision-engineered, double-beam optical instrument designed for high-stability absorbance, transmittance, and reflectance measurements across the ultraviolet, visible, and near-infrared spectral regions (190–1100 nm). Based on a Czerny-Turner monochromator architecture with a fixed-slit grating and low-stray-light optics, the Lambda 40 delivers reproducible photometric performance essential for quantitative analysis in regulated and research environments. Its double-beam design continuously corrects for source drift and detector sensitivity fluctuations, enabling robust baseline stability over extended acquisition periods—critical for kinetic assays, concentration profiling, and method validation workflows. This refurbished unit has undergone full functional recalibration and optical alignment at SpectraLab Scientific’s certified refurbishment facility, including wavelength accuracy verification using NIST-traceable holmium oxide and didymium filters, and photometric linearity assessment per ASTM E275.

Key Features

• Double-beam optical path with real-time reference beam compensation for enhanced long-term stability
• High-efficiency deuterium and tungsten-halogen lamps with automatic source switching at ~350 nm
• Fixed 2-nm spectral bandwidth (SBW), optimized for resolution/sensitivity balance in routine QC applications
• Integrated 128 × 64 pixel LCD display and membrane keypad for standalone operation—no PC required for basic measurements
• USB interface supporting direct data export to CSV or ASCII formats; compatible with legacy PerkinElmer UV WinLab™ v3.x software (Windows 7/10 32-bit)
• Robust mechanical design with temperature-compensated optics and shock-absorbing base—engineered for laboratory longevity
• Pre-installed firmware version 3.10, supporting GLP-compliant audit trail logging when used with compliant software environments

Sample Compatibility & Compliance

The Lambda 40 accommodates standard 10-mm square quartz or glass cuvettes (pathlengths: 1–50 mm via optional holders), as well as integrating spheres (optional accessory) for diffuse reflectance of solids and powders. It supports liquid, solution, thin-film, and suspended particulate samples. The system complies with key international standards for spectrophotometric validation: ASTM E275 (test methods for spectrophotometers), ISO 6223 (performance requirements for UV-Vis instruments), and USP (spectrophotometric absorption measurements). When operated under documented procedures—including regular calibration with certified reference materials (e.g., potassium dichromate in 0.005 M H₂SO₄ for absorbance accuracy)—the instrument meets data integrity expectations aligned with FDA 21 CFR Part 11 principles for non-electronic-record-dependent use cases.

Software & Data Management

While fully functional in stand-alone mode, the Lambda 40 integrates seamlessly with PerkinElmer’s UV WinLab™ software suite for advanced spectral acquisition, multi-component quantitation, kinetics modeling, and report generation. All refurbished units ship with licensed software media and activation keys. Data files are stored in vendor-native .spc format and exportable to universal formats (CSV, TXT, XML) for LIMS integration or third-party analysis (e.g., MATLAB, Python pandas). Audit trail functionality—including operator ID, timestamp, parameter changes, and measurement history—is enabled when UV WinLab™ is configured in “compliance mode” with user access controls and electronic signature prompts. Raw spectral data retain full wavelength-intensity fidelity without interpolation or compression.

Applications

• Quantitative determination of nucleic acids and proteins (A260/A280 ratios, Bradford/Lowry assays)
• Pharmaceutical assay development and release testing per ICH Q2(R2) guidelines
• Water quality analysis (nitrate, phosphate, COD via colorimetric methods)
• Pigment concentration profiling in coatings, inks, and food colorants
• Stability-indicating methods for forced degradation studies
• Educational laboratories requiring reliable, serviceable instrumentation with documented traceability

FAQ

Is this Lambda 40 unit factory-certified or independently refurbished?
This instrument was refurbished by SpectraLab Scientific—a ISO 9001-accredited third-party service provider specializing in analytical instrumentation. Refurbishment includes full optical alignment, lamp replacement (if aged >1,000 h), detector dark-current characterization, and compliance verification against ASTM E275. A detailed Certificate of Refurbishment is provided.
Does the 6-month warranty cover parts, labor, and lamp replacement?
Yes—the warranty covers all components, labor, and consumables including the deuterium and tungsten-halogen lamps, subject to normal laboratory use conditions and adherence to recommended maintenance intervals.
Can this Lambda 40 be upgraded to support scanning kinetics or multi-cell changers?
The Lambda 40 hardware platform does not support automated sample changers or rapid-scanning accessories; those capabilities require Lambda 650/950-series instrumentation. However, manual kinetics can be performed via time-point acquisition in UV WinLab™.
What documentation accompanies the instrument?
Each unit ships with a Refurbishment Report, Photometric Performance Verification Log, NIST-traceable calibration certificate (wavelength and absorbance), USB cable, power cord, operator’s manual (digital PDF), and one set of matched 10-mm quartz cuvettes.
Is remote technical support available post-purchase?
Yes—SpectraLab Scientific provides complimentary email-based technical assistance for 90 days post-delivery, plus optional extended support contracts covering preventive maintenance, software updates, and application troubleshooting.