Alltech Prevail C18 Reconditioned HPLC Column
| Origin | Canada |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | Alltech Prevail C18 |
| Price Range | USD 150–7,500 (FOB) |
| Product Category | Chromatography Column |
| Service Life | 4–5 years |
| Warranty | 6 months |
| Part Number | 99210 |
| Dimensions | 250 mm × 2.0 mm ID |
| Particle Size | 5 µm |
| Pore Size | 120 Å |
| Phase | Octadecylsilane (C18) |
Overview
The Alltech Prevail C18 reconditioned HPLC column is a high-performance reversed-phase chromatographic column engineered for robust separation of small-molecule analytes in pharmaceutical, environmental, and quality control laboratories. Built on fully porous silica particles with a 120 Å pore diameter and 5 µm particle size, this 250 mm × 2.0 mm column delivers consistent retention, peak symmetry, and long-term stability under standard aqueous/organic mobile phase conditions (e.g., water/acetonitrile or water/methanol gradients). Its bonded phase consists of densely grafted octadecylsilane (C18), providing strong hydrophobic interactions while maintaining compatibility with low-pH mobile phases (pH 2–8). As a reconditioned unit, it undergoes rigorous post-use validation—including pressure testing, backpressure profiling, efficiency assessment (N > 8,000 plates/m for uracil), and asymmetry factor evaluation (As < 1.3)—to ensure conformance with ICH Q5A and USP system suitability criteria.
Key Features
- Reconditioned and functionally validated per ISO/IEC 17025-aligned internal protocols
- Consistent batch-to-batch reproducibility verified via retention time RSD ≤ 0.3% (n = 5 injections)
- Stable baseline performance across 1,000+ injections when operated within recommended flow rates (0.2–0.5 mL/min for 2.0 mm ID)
- Low metal content silica support minimizes chelation-related tailing for basic compounds
- Endcapped to reduce silanol activity—critical for amine-containing analytes such as beta-blockers and alkaloids
- Compatible with standard HPLC and UHPLC systems operating up to 400 bar
Sample Compatibility & Compliance
This column supports a broad range of analytes including organic acids, aromatic hydrocarbons, peptides (≤ 2 kDa), nucleosides, and polar pharmaceuticals. It complies with common regulatory frameworks governing analytical method transfer and instrument qualification, including FDA 21 CFR Part 11 requirements for electronic records generated during column qualification runs. While not supplied with full GMP documentation by default, complete IQ/OQ documentation packages—including column logbooks, pressure vs. time curves, and system suitability reports—are available upon request for GLP- and GMP-regulated environments. The column meets ASTM D7213 (for hydrocarbon analysis) and USP Class L1 specifications for C18 columns.
Software & Data Management
No proprietary software is required for operation; the column integrates seamlessly with all major chromatography data systems (CDS), including Thermo Chromeleon, Agilent OpenLab CDS, Waters Empower, and Shimadzu LabSolutions. Raw pressure, retention time, and peak area data exported from these platforms can be imported into Spectralab’s optional Column Health Dashboard—a web-based analytics tool that tracks cumulative usage metrics, identifies early signs of frit blockage or phase collapse, and generates predictive maintenance alerts based on historical injection counts and mobile phase composition logs. Audit trails comply with ALCOA+ principles and support inspection readiness.
Applications
- Pharmaceutical QC testing: assay and related substances analysis per USP monographs (e.g., metformin HCl, atorvastatin calcium)
- Environmental monitoring: detection of PAHs and chlorinated pesticides in soil extracts per EPA Method 8330B
- Food safety: quantification of mycotoxins (aflatoxin B1, ochratoxin A) following AOAC 2005.02
- Academic research: method development for natural product isolation and metabolite profiling
- Bioanalytical support: plasma protein binding studies using ultrafiltration-coupled HPLC workflows
FAQ
Is this column shipped with a certificate of reconditioning?
Yes—each unit includes a traceable Certificate of Reconditioning listing test parameters, pass/fail status, and technician signature.
Can it be used for method transfer from a new Alltech Prevail C18 column?
Yes, provided system suitability criteria are met; we recommend initial bracketing with reference standards and reporting k’ and α values for comparability assessment.
What mobile phase additives are compatible?
0.1% formic acid, 0.1% trifluoroacetic acid, and ammonium acetate buffers (5–50 mM) are fully supported; avoid phosphate buffers above pH 7.5 to prevent silica dissolution.
Does the 6-month warranty cover performance drift?
Yes—the warranty includes replacement or credit if resolution, plate count, or tailing factor deviates beyond ±15% of initial validation values under documented operating conditions.
Is column regeneration possible after contamination?
Standard regeneration protocols (e.g., 20 column volumes each of water → acetonitrile → isopropanol → dichloromethane → acetonitrile → water) are effective for most non-covalent fouling; irreversible damage requires professional refurbishment assessment.
