Agilent 1200 Series HPLC System with G1315B Diode Array Detector

Overview

The Agilent 1200 Series HPLC System is a high-performance, modular liquid chromatography platform engineered for precision, robustness, and method transfer reliability in regulated and research environments. Built upon Agilent’s proven low-dispersion fluidic architecture, this system employs dual-piston reciprocating pumps (G1311A) delivering pulse-free flow across a wide range of solvents and viscosities. The G1315B Diode Array Detector (DAD) provides full-spectrum UV-Vis detection from 190–950 nm with 1 nm resolution, enabling peak purity assessment, spectral library matching, and multi-wavelength quantitation without re-injection. As a pre-owned instrument fully refurbished by certified technicians, this unit retains original Agilent hardware integrity—including stainless-steel flow paths, temperature-stabilized optics, and pressure-rated solvent delivery—and meets functional specifications aligned with ASTM E2657-20 (Standard Practice for Verification of HPLC Systems) and ISO/IEC 17025 traceability requirements for calibration verification.

Key Features

• Modular G1200-series architecture supporting gradient elution, isocratic operation, and method scalability from analytical to semi-preparative workflows
• G1311A Quaternary Pump with active solvent selection, flow accuracy ±0.1% RSD, and pressure operating range up to 400 bar
• G1329A Autosampler featuring 100-position vial capacity, programmable injection volumes (0.1–100 µL), and needle wash capability to minimize carryover
• G1316A Thermostatted Column Compartment maintaining temperature stability ±0.15 °C over 5–80 °C range, critical for retention time reproducibility
• G1315B Diode Array Detector with deuterium/tungsten dual-lamp source, 1024-pixel linear array, and real-time spectral acquisition at up to 80 Hz
• Integrated control via Agilent ChemStation software (included) with audit trail, electronic signatures, and 21 CFR Part 11 compliance options enabled
• Fully refurbished with replaced seals, gaskets, lamp, and detector cell; verified performance using NIST-traceable caffeine and uracil test mixtures

Sample Compatibility & Compliance

This system accommodates standard 3.0–4.6 mm ID analytical columns (including reversed-phase C18, HILIC, and ion-exchange chemistries) and supports mobile phases ranging from aqueous buffers to high-acetonitrile or methanol gradients. It complies with key regulatory frameworks for quality-controlled laboratories: all hardware and firmware revisions are documented per GLP/GMP documentation standards; baseline noise and drift metrics meet USP chromatographic system suitability criteria; and the refurbished configuration includes full service history logs and calibration certificates traceable to NIST standards. Optional IQ/OQ documentation packages are available upon request to support installation qualification and operational qualification in pharmaceutical or clinical testing labs.

Software & Data Management

Controlled exclusively through Agilent ChemStation Rev. A.10.02 or later (licensed and pre-installed), the system supports complete data acquisition, processing, reporting, and archiving workflows. ChemStation implements secure user access levels, electronic signature enforcement, and automated audit trail generation—fully compatible with FDA 21 CFR Part 11 requirements when configured with Windows domain authentication and encrypted database storage. Raw data files (.D files) are stored in vendor-neutral formats suitable for long-term archival and third-party reprocessing. Integration with LIMS platforms (e.g., LabWare, Thermo SampleManager) is supported via standard OLE/COM and ASCII export protocols.

Applications

The Agilent 1200 Series with G1315B DAD is routinely deployed in pharmaceutical QC for assay validation, impurity profiling, and stability-indicating methods per ICH Q2(R2); in environmental labs for PAH, pesticide, and pharmaceutical residue analysis per EPA Method 8330B and ISO 17993; in food safety for mycotoxin and vitamin quantitation; and in academic research for natural product fractionation and metabolite identification. Its spectral fidelity enables post-run peak deconvolution and co-elution resolution—particularly valuable in complex matrices such as plasma extracts, plant hydrolysates, or fermentation broths.

FAQ

Is this system eligible for extended warranty or service contracts?
Yes—optional 12- or 24-month comprehensive service agreements are available, covering parts, labor, preventive maintenance, and priority remote diagnostics.
Does the refurbishment include replacement of the DAD lamp and flow cell?
Yes—both the deuterium and tungsten lamps were replaced, and the quartz flow cell was inspected, cleaned, and pressure-tested prior to system validation.
Can this HPLC be upgraded to include a mass spectrometer interface?
The G1315B DAD module is not directly compatible with MS coupling; however, the G1312B binary pump and G1322A degasser in this series support LC-MS integration when paired with an appropriate interface (e.g., Agilent 6100 Series MSD) and modified plumbing.
What documentation accompanies the system?
You receive a full refurbishment report, calibration certificate, system suitability test results, ChemStation license documentation, and OEM operation manuals in English.
Is remote installation support provided?
Yes—our application engineers offer virtual commissioning assistance, including network configuration, software activation, and initial system suitability testing guidance.