Agilent 1200 Series Used High-Performance Liquid Chromatograph (HPLC) System
| Brand | Agilent Technologies |
|---|---|
| Origin | USA |
| Model | 1200 |
| Product Category | Chromatography |
| Usage Duration | 5–7 years |
| Warranty | 6 months |
| Compliance | Fully Tested & Certified per ISO/IEC 17025 Traceable Procedures |
| Software | OpenLAB CDS (ChemStation-compatible) |
| Detector Type | Diode Array Detector (DAD) |
| Autosampler Temperature Range | 4–40 °C |
| Injection Volume Range | 0.1–100 µL |
| Pump Configuration | Quaternary or Binary High-Pressure Gradient System |
| Column Oven | Thermostatically Controlled (Ambient to 80 °C) |
| Degasser | Integrated Vacuum Membrane Degasser |
Overview
The Agilent 1200 Series is a modular, high-performance liquid chromatography (HPLC) platform engineered for precision, reproducibility, and long-term operational stability in regulated and research laboratory environments. Based on proven low-dispersion fluidic architecture and dual-lamp diode array detection, the system delivers robust baseline stability, high photometric accuracy, and excellent retention time repeatability—critical parameters for method transfer, QC release testing, and stability-indicating assays. Its modular design supports flexible configuration: individual modules—including quaternary pumps (G1311A), binary high-pressure pumps (G1312A), thermostatted autosamplers (G1329A/G1367D), column ovens (G1316A/G1316X), and DAD detectors (G1315B/G1315X)—are fully interoperable and retain original firmware calibration certificates post-refurbishment. All units undergo full functional validation against Agilent’s Factory Acceptance Test (FAT) protocol, including pressure integrity checks, gradient accuracy verification (±0.2% RSD), dwell volume measurement, and detector linearity assessment across 190–950 nm.
Key Features
- Modular architecture enabling scalable configurations—from entry-level isocratic systems to fully automated GMP-compliant gradient platforms
- High-pressure quaternary pump (G1311A) with integrated vacuum degasser (G1379B), delivering flow accuracy ≤ ±0.1% RSD and pulse dampening < 0.1% RMS
- Thermostatted autosampler (G1329A) with 4–40 °C temperature control, 100-position tray capacity, and programmable needle wash cycles to minimize carryover (< 0.005%)
- Diode array detector (G1315B) featuring dual tungsten-halogen and deuterium lamps, real-time spectral acquisition (1–8 nm resolution), and photometric noise < ±0.5 × 10⁻⁵ AU at 254 nm
- Column oven (G1316A) with precise temperature control (±0.1 °C) from ambient +5 °C to 80 °C, supporting method robustness under variable ambient conditions
- Fully validated firmware and hardware compatibility with Agilent OpenLAB CDS 2.x and legacy ChemStation software (licensed separately)
Sample Compatibility & Compliance
The Agilent 1200 Series accommodates standard 3.0–4.6 mm ID analytical columns (up to 250 mm length) and microbore formats (1.0–2.1 mm ID). It supports aqueous, organic, and mixed mobile phases—including TFA, HFBA, and ion-pair reagents—within pressure limits up to 400 bar. All refurbished systems are verified for compliance with ASTM E2656 (Standard Guide for HPLC System Performance Verification), USP (Chromatography), and ICH Q2(R2) guidelines for analytical method validation. Audit trails, electronic signatures, and instrument qualification documentation (IQ/OQ/PQ templates) are provided upon request to support GLP, GMP, and FDA 21 CFR Part 11 readiness.
Software & Data Management
Systems operate natively with Agilent OpenLAB CDS, offering full method development, sequence management, peak integration, and report generation. The software supports secure user role assignment, electronic lab notebook (ELN) integration, and automated data backup to network drives or NAS devices. All instruments ship with factory-reset firmware and include a software license key for ChemStation compatibility (where applicable). Raw data files (.D files) are fully compliant with AIA/ANDI standards and exportable to third-party processing tools (e.g., Chromeleon, Empower) via ASCII conversion utilities.
Applications
This platform is routinely deployed in pharmaceutical quality control (assay, related substances, dissolution), environmental analysis (PAHs, pesticides, PFAS), food safety (mycotoxins, preservatives, vitamins), and academic research (natural product purification, polymer characterization, metabolomics sample prep). Its high sensitivity and spectral fidelity make it especially suitable for stability-indicating methods where peak purity assessment via peak tracking and library matching is required. The system’s modularity also allows integration with preparative modules (G1361A pump + G1364X fraction collector) for semi-preparative scale-up.
FAQ
What documentation accompanies each refurbished Agilent 1200 system?
Each unit ships with a Certificate of Conformance, full FAT report (including pressure, gradient, and detector performance metrics), calibration logs traceable to NIST standards, and a 6-month limited warranty covering parts and labor.
Can this system be upgraded to support UHPLC capabilities?
No—the 1200 Series is designed for HPLC pressure ranges (max 400 bar); UHPLC functionality requires the 1290 Infinity II platform (1300 bar). However, compatible 2.7 µm core-shell columns can be used to enhance resolution without exceeding system pressure limits.
Is remote diagnostics supported?
Yes—when connected to a local network, the system supports Agilent’s Remote Monitoring Service (RMS) for real-time status alerts, predictive maintenance notifications, and secure technician-assisted troubleshooting.
Are spare parts and consumables available?
All OEM modules, seals, gaskets, and lamp assemblies (e.g., G1315-60101 DAD lamp, G1311-60013 pump seal kit) remain in active production and are stocked for immediate dispatch.
Does the warranty cover software licensing?
The hardware warranty does not include software licenses; however, OpenLAB CDS evaluation licenses are provided, and commercial licenses may be procured directly from Agilent or authorized resellers.
