Agilent 1200 Series HPLC System (Refurbished)

Overview

The Agilent 1200 Series HPLC System is a high-performance liquid chromatography platform engineered for robustness, method transfer fidelity, and long-term operational stability in regulated and research laboratory environments. Based on Agilent Technologies’ proven quaternary solvent delivery architecture, this refurbished system employs low-dispersion flow paths, precise gradient formation (0.1%–100% B in ≤10 µL dwell volume), and pressure-compensated pumping to ensure reproducible retention times and peak shape integrity across diverse mobile phase compositions. The system operates under standard isocratic or gradient elution modes and supports reversed-phase, normal-phase, ion-exchange, and size-exclusion chromatographic separations. Designed for compliance-ready operation, its modular architecture allows seamless integration into existing lab workflows and facilitates future upgrades to LC/MS coupling or automated sample preparation interfaces.

Key Features

• Refurbished and fully validated by SpectraLab Scientific — each unit undergoes comprehensive functional testing including pump linearity (±0.5% RSD), detector baseline noise (< ±2.5 × 10⁻⁵ AU), wavelength accuracy (±1 nm at 254 nm), and autosampler precision (≤0.5% RSD for 10 µL injections).
• G1311A Quaternary Pump with integrated pulse dampening and real-time pressure monitoring (0–400 bar range), enabling stable flow rates from 0.001 to 10.0 mL/min with ≤0.1% RSD.
• G1379B Vacuum Degasser with four-channel capacity and automatic self-purge cycle, minimizing baseline drift and bubble-induced signal artifacts.
• G1329A/G1367B Autosampler featuring refrigerated sample cooling (4–40 °C), needle wash station, and programmable injection volumes (0.1–100 µL) with low carryover (<0.005%).
• G1316A Column Oven with temperature control from ambient +5 °C to 80 °C (±0.15 °C stability), supporting method robustness across thermal gradients.
• G1315B Diode Array Detector (DAD) offering full-spectrum acquisition (190–800 nm), photometric linearity up to 2.5 AU, and real-time spectral deconvolution capabilities.
• G1330B Thermostatted Column Compartment providing independent temperature regulation for column modules, ensuring consistent retention time alignment during multi-method sequences.

Sample Compatibility & Compliance

The Agilent 1200 Series accommodates standard 2.1–4.6 mm ID analytical columns and supports mobile phases ranging from aqueous buffers (pH 1.5–12.5) to high-organic solvents (e.g., acetonitrile, methanol, THF). All fluidic components are chemically resistant to common HPLC solvents and compatible with USP , ASTM D7214, and ISO 17025-compliant method validation protocols. The system meets mechanical and electrical safety requirements per IEC 61010-1 and carries CE marking. While the refurbished units do not include original factory calibration certificates, full traceable calibration documentation—aligned with NIST-traceable standards—is provided upon request for GLP/GMP-regulated applications.

Software & Data Management

The system is compatible with Agilent ChemStation Rev. B.04.03 or later, supporting audit trail-enabled data acquisition, electronic signatures, and 21 CFR Part 11-compliant user access controls when deployed with appropriate server infrastructure. Raw data files (.D format) are natively supported for reprocessing, peak integration, and reporting. Optional integration with OpenLAB CDS enables centralized instrument management, method versioning, and automated report generation compliant with FDA and EMA submission guidelines. All refurbished systems ship with licensed software media and activation keys; network licensing options are available upon consultation.

Applications

This HPLC platform serves routine QC/QA analysis in pharmaceutical, food safety, environmental, and polymer laboratories—including assay quantitation of active pharmaceutical ingredients (APIs), residual solvent testing per ICH Q3C, pesticide residue screening in agricultural commodities, organic acid profiling in fermentation broths, and molecular weight distribution analysis of synthetic polymers. Its reliability and method reproducibility make it particularly suitable for compendial testing (USP, EP, JP), stability-indicating assays, and reference standard qualification workflows.

FAQ

Is this system eligible for extended warranty or service contracts?
Yes—extended service plans (12–36 months) covering preventive maintenance, parts replacement, and remote diagnostics support are available through authorized Agilent service partners.
Can the system be upgraded to support mass spectrometry detection?
Yes—the G1312B binary pump (optional upgrade) and G1316B column oven provide enhanced compatibility with LC/MS interfaces; API source integration requires additional hardware configuration and validation.
Are original Agilent consumables and spare parts still available?
All major consumables—including pump seals, purge valves, detector flow cells, and autosampler rotor seals—are commercially available through Agilent’s global distribution network and third-party certified suppliers.
Does the refurbishment process include firmware updates?
Yes—each unit receives firmware update to the latest stable revision supported by its hardware generation, verified via diagnostic boot sequence and module handshake tests.
What documentation accompanies the system?
Each shipment includes a Certificate of Refurbishment, functional test report, calibration summary, user manuals (English), and software installation media with valid license key.