Agela PrepMaster Modular Flash Purification System
| Brand | Agela |
|---|---|
| Origin | Tianjin, China |
| Manufacturer Type | Authorized Distributor |
| Product Origin | Domestic (China) |
| Model Series | Multiple Catalog Numbers |
| Pricing | Available Upon Request |
Overview
The Agela PrepMaster Modular Flash Purification System is a high-performance, benchtop-scale preparative chromatography platform engineered for synthetic organic chemists, medicinal chemistry labs, and process development teams requiring rapid, reproducible compound isolation and purification. Unlike conventional fixed-configuration flash systems, the PrepMaster employs a modular architecture based on low-dispersion, high-pressure gradient-capable dual-pump modules, UV/Vis detection (200–400 nm), and intelligent fraction collection—all integrated via a dedicated control interface. Its core separation principle relies on normal-phase and reversed-phase flash chromatography, operating within pressure ranges up to 200 bar and flow rates scalable from 1 mL/min to 120 mL/min depending on column dimensions and solvent compatibility. The system supports both isocratic and linear gradient elution, enabling method transfer from analytical HPLC to preparative scale with minimal re-optimization.
Key Features
- Modular Scalability: Users select and combine pump modules (single or dual gradient), detector options (fixed-wavelength or variable-wavelength UV/Vis), fraction collectors (3-position or 4-dimensional robotic arm), and column switching valves—without proprietary lock-in.
- Reversible Flow Path Architecture: Integrated valve manifold allows seamless switching between normal-phase (e.g., hexane/EtOAc) and reversed-phase (e.g., water/acetonitrile) modes without hardware reconfiguration or tubing disassembly.
- 4-Dimensional Fraction Collection: Robotic collector with X/Y/Z-axis translation plus rotary sample tube rack indexing enables precise, time- or peak-triggered collection across >120 vials per run; compatible with standard 13 mm × 100 mm or 16 mm × 150 mm glass/tube formats.
- Intelligent Control Interface: Touch-enabled software provides real-time chromatogram display, peak integration, threshold-based fraction triggering, method storage (≥500 protocols), and audit-trail logging compliant with GLP documentation requirements.
- High-Capacity Sample Introduction: Equipped with dual-loop injection module supporting up to 5 mL loop volume; optional continuous cross-injection mode permits uninterrupted sample loading during ongoing purification runs.
- Chemical Compatibility & Safety: Fluidic path constructed from PEEK, stainless steel (316L), and sapphire components; rated for common organic solvents including DCM, THF, DMF, and alcohols; includes leak detection and overpressure shutdown protocols.
Sample Compatibility & Compliance
The PrepMaster accommodates a broad range of sample types—including crude reaction mixtures, natural product extracts, peptide intermediates, and small-molecule APIs—with load capacities from sub-milligram to multi-gram scale depending on column format (4 g to 340 g silica cartridges or custom-packed columns). It meets fundamental design and operational criteria aligned with ISO 9001 quality management practices. While not certified as a Class I medical device, its software supports 21 CFR Part 11-compliant user access controls, electronic signatures, and immutable audit trails when deployed in regulated environments (e.g., API purification under GMP guidelines). All hardware components comply with CE electromagnetic compatibility (EMC) Directive 2014/30/EU and Low Voltage Directive 2014/35/EU.
Software & Data Management
Control and data acquisition are managed through Agela’s PrepMaster Studio software, a Windows-based application offering method editor, real-time chromatogram overlay, baseline correction algorithms, and customizable reporting templates (PDF/CSV export). Raw data files are stored in vendor-neutral .chm format, ensuring long-term readability. Software supports role-based user permissions (administrator, operator, reviewer), session locking, and automatic backup to network drives or local SSD. For laboratories implementing LIMS integration, the system provides OPC UA server capability and configurable ASCII output for third-party data ingestion.
Applications
- Purification of synthetic intermediates in drug discovery campaigns (e.g., Suzuki couplings, amide bond formations)
- Isolation of bioactive compounds from plant or microbial extracts
- Removal of metal catalysts (Pd, Ni) and phosphine ligands post-coupling reactions
- Desalting and buffer exchange of peptides prior to lyophilization
- Scale-up support for route scouting—bridging analytical HPLC methods to 10–100 g purification batches
- Quality control of custom-synthesized reference standards prior to NMR or MS characterization
FAQ
Does the PrepMaster support method transfer from analytical HPLC?
Yes—the system accepts retention time and gradient slope inputs from analytical methods and automatically scales flow rate, injection volume, and column dimensions using built-in scaling algorithms.
Can it be operated under inert atmosphere for air-sensitive compounds?
While the base configuration is ambient-air compatible, optional nitrogen-purged sample loops, sealed fraction collector enclosures, and solvent degassing modules are available for oxygen- or moisture-sensitive applications.
Is remote monitoring supported?
The PrepMaster Studio software includes secure remote desktop access capability via corporate VPN; no cloud-hosted data storage is used by default.
What maintenance intervals are recommended?
Pump seals should be inspected every 500 hours of operation; UV lamp replacement is advised at 2,000 hours; annual calibration verification of flow accuracy and detector linearity is recommended per ISO/IEC 17025 guidance.
