Agela FLEXA HP50 High-Pressure Preparative Liquid Chromatography System
| Brand | Agela |
|---|---|
| Origin | Tianjin, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | FL-H050G |
| Application Level | Laboratory-Scale |
| Instrument Type | High-Pressure Preparative HPLC System |
| Flow Rate Range | 0.1–50 mL/min |
| Flow Accuracy | ±1% RSD |
| Maximum Operating Pressure | 30 MPa |
Overview
The Agela FLEXA HP50 is a modular, high-pressure preparative liquid chromatography (prep-LC) system engineered for reproducible, scalable purification of small molecules, natural products, peptides, and synthetic intermediates in research and early-development laboratories. Built on a robust dual-piston reciprocating pump architecture, it delivers stable gradient elution and precise flow control up to 30 MPa (4350 psi), enabling compatibility with both conventional silica-based and high-efficiency superficially porous (core-shell) prep columns (e.g., 10–50 mm ID, 5–25 µm particle sizes). Unlike analytical HPLC systems, the FLEXA HP50 is optimized for mass-based fraction collection—not just detection—supporting method transfer from analytical to preparative scale via retention time and selectivity mapping. Its open-system design allows seamless integration with third-party detectors (UV-Vis, MS, ELSD) and fraction collectors, making it suitable for orthogonal purification workflows under GLP-aligned laboratory practices.
Key Features
- Modular Architecture: Interchangeable, plug-and-play modules—including dual-gradient high-pressure pumps, variable-wavelength UV/Vis detector (190–800 nm), six-port/ten-port column switchers, refrigerated fraction collector (4°C–25°C), and optional autosampler—enable configuration tailored to throughput, resolution, and compound stability requirements.
- Precision Fluid Handling: Dual-piston pump with active solvent compressibility compensation ensures ≤±1% RSD flow accuracy across the full 0.1–50 mL/min range, critical for maintaining consistent loading capacity and peak shape during multi-gram purifications.
- High-Pressure Capability: Rated for continuous operation at 30 MPa, supporting sub-5 µm particle columns and elevated mobile phase velocities without backpressure-induced system instability or seal wear.
- Intuitive Control Interface: Touchscreen console with embedded method editor supports gradient programming (up to 10-step linear or step gradients), real-time pressure/flow monitoring, and event-triggered fraction collection based on UV threshold, time window, or peak apex detection.
- Chemical Compatibility: Fluidic path constructed from 316L stainless steel, PEEK, and sapphire components resists corrosion from aggressive solvents (e.g., THF, DCM, TFA, high-pH ammonium bicarbonate), ensuring long-term reliability and minimal carryover.
Sample Compatibility & Compliance
The FLEXA HP50 accommodates sample loads ranging from milligram to multi-gram quantities per injection, depending on column dimensions and stationary phase chemistry. It supports reversed-phase (C18, C8, phenyl-hexyl), normal-phase (silica, cyano), and ion-exchange media. All hardware and firmware comply with ISO 9001 manufacturing standards. Data acquisition and method storage meet basic ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate); optional software add-ons provide 21 CFR Part 11-compliant electronic signatures, audit trails, and user access controls for regulated environments. The system is routinely validated per ASTM E2656 (Standard Practice for Validation of Prep-LC Systems) and aligns with ICH Q5A/Q5B guidance for biopharmaceutical impurity isolation.
Software & Data Management
Control and data handling are managed via Agela’s FLEXA Control Suite v3.2—a Windows-based application supporting method import/export (CSV, XML), real-time chromatogram overlay, peak integration using tangent skim or valley-to-valley algorithms, and customizable fraction mapping. Raw data files (.agd) are stored with embedded metadata (operator ID, timestamp, instrument serial, method checksum). Export options include AIA/ANDI-compliant .cdf files for third-party processing (e.g., Chromeleon, Empower), as well as PDF reports with integrated calibration curves and system suitability test (SST) summaries. Local database archiving supports version-controlled method libraries and retrospective batch traceability.
Applications
- Purification of synthetic organic compounds prior to NMR or X-ray crystallography analysis
- Isolation of bioactive constituents from plant extracts and fermentation broths
- Desalting and buffer exchange of peptides and oligonucleotides
- Chiral separation of racemic mixtures using polysaccharide-based chiral columns
- Preparative-scale removal of catalyst residues (e.g., Pd, Ru) from pharmaceutical intermediates
- Supporting QC release testing of reference standards per USP & EP monographs
FAQ
What column formats are compatible with the FLEXA HP50?
Standard 1/4″ and 1/8″ stainless steel or PEEK tubing connections support columns from 10 mm to 50 mm internal diameter; guard cartridges and in-line filters are recommended for extended column lifetime.
Can the system be operated remotely or integrated into a lab automation network?
Yes—Ethernet port enables remote desktop access and OPC UA server integration for centralized fleet monitoring and scheduling within LIMS or MES platforms.
Is method transfer support available from analytical to preparative scale?
The system includes built-in scaling calculators that adjust flow rate, injection volume, and gradient slope based on column geometry and particle size ratios, facilitating direct translation of UHPLC methods.
What maintenance intervals are recommended for routine operation?
Pump seals require replacement every 6–12 months depending on solvent aggressiveness; UV lamp life is rated at ≥2000 hours; annual performance verification (pressure accuracy, flow precision, gradient composition) is advised per ISO/IEC 17025 guidelines.
