Hanbon Bio-Oligo100 Automated DNA/RNA Synthesizer
| Brand | Hanbon |
|---|---|
| Origin | Jiangsu, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Origin | Domestic (China) |
| Model | 100 |
| Price Range | USD 112,000 – 140,000 (FOB Jiangsu) |
| Temperature Range | 10–40 °C |
| Reaction Units | 1 |
| Synthesis Scale | 50 μmol – 9 mmol |
Overview
The Hanbon Bio-Oligo100 is a fully automated solid-phase oligonucleotide synthesizer engineered for high-fidelity, scalable DNA and RNA synthesis in research, pharmaceutical development, and diagnostic reagent manufacturing. It operates on the phosphoramidite chemistry platform—a standardized, iterative four-step cycle (detritylation, coupling, capping, and oxidation) executed under inert atmosphere control to ensure sequence integrity and coupling efficiency >99.3% per step at optimal scales. Designed for reproducible synthesis across micromolar to millimolar scales, the system supports both standard and modified nucleoside phosphoramidites—including 2′-O-methyl, LNA, phosphorothioate, and fluorescent-dye–labeled monomers—enabling production of antisense oligonucleotides (ASOs), siRNA, aptamers, molecular beacons, and NGS library adapters. Its single-reaction-channel architecture prioritizes operational simplicity and maintenance accessibility without compromising scalability or chemical compatibility.
Key Features
- Single-channel, high-capacity synthesis platform supporting scales from 50 μmol to 9 mmol—ideal for preclinical batch synthesis, GMP-aligned process development, and large-scale probe manufacturing.
- Integrated temperature-controlled reagent delivery manifold with precise ambient regulation (10–40 °C), minimizing solvent volatility and amidite degradation during extended synthesis runs.
- Modular column holder accommodating industry-standard 10 mL, 50 mL, and 100 mL synthesis columns—enabling seamless transition from analytical-scale validation to pilot-batch production.
- Intelligent synthesis software with customizable method templates, real-time reaction monitoring (via pressure/flow sensors), and built-in error logging compliant with ALCOA+ data integrity principles.
- Chemical-resistant fluidic path constructed from PFA, PTFE, and electropolished 316L stainless steel—ensuring compatibility with acetonitrile, dichloroacetic acid, iodine/water/pyridine, and other aggressive reagents used in phosphoramidite chemistry.
- Onboard inert gas purging (argon or nitrogen) for all reagent lines and reaction vessels, maintaining <10 ppm O₂ exposure throughout synthesis to prevent oxidation side reactions.
Sample Compatibility & Compliance
The Bio-Oligo100 accommodates a broad spectrum of solid supports—including controlled-pore glass (CPG), polystyrene (PS), and macroporous polystyrene (MPPS)—with pore sizes ranging from 500 Å to 3000 Å. It supports synthesis of DNA (up to 200-mer), RNA (up to 100-mer), and chimeric sequences incorporating 2′-F, 2′-OMe, and unlocked nucleic acid (UNA) modifications. The instrument meets CE marking requirements for laboratory equipment (2014/30/EU EMC Directive and 2014/35/EU LVD Directive) and conforms to ISO 13485:2016 design controls for medical device-related reagent synthesis. While not certified as a Class I medical device itself, its output is routinely validated for use in IVD assay development under CLIA and IVDR frameworks.
Software & Data Management
The Hanbon SynthControl™ software provides role-based user access, electronic batch records (EBR), and full audit trail functionality compliant with FDA 21 CFR Part 11 Annex 11 requirements. All synthesis methods, parameter logs, reagent lot tracking, and alarm events are timestamped, digitally signed, and exportable in CSV or PDF format. The system supports optional integration with LIMS via ASTM E1578-compliant API endpoints. Data retention policies align with GLP and GMP Annex 11 expectations—supporting ≥25-year archival of raw synthesis metadata when deployed with validated network storage infrastructure.
Applications
- Preclinical development of therapeutic oligonucleotides, including splice-switching ASOs and RNAi triggers for IND-enabling toxicology studies.
- Production of high-purity molecular diagnostic probes for qPCR, digital PCR, and hybrid capture-based NGS workflows.
- Custom synthesis of labeled primers and adapters for next-generation sequencing library preparation kits.
- Academic and core facility support for structural biology (e.g., crystallization-grade RNA constructs) and synthetic biology (e.g., gene fragment assembly).
- Process optimization and scale-up studies for contract development and manufacturing organizations (CDMOs) targeting commercial-grade oligonucleotide APIs.
FAQ
What synthesis scale ranges does the Bio-Oligo100 support?
It delivers consistent performance from 50 μmol up to 9 mmol per synthesis run—covering early-stage screening through clinical trial material production.
Is the system compatible with RNA synthesis using 2′-ACE or TOM protection schemes?
Yes; the fluidic architecture and reagent delivery timing are configurable for both standard DMT-on-DNA and acid-labile RNA protecting group strategies, including 2′-TBDMS and 2′-ACE deprotection protocols.
Can the instrument be integrated into an automated lab environment?
It features RS-232, Ethernet, and optional OPC UA connectivity for scheduling via MES or robotic sample-handling platforms.
Does the system include validation documentation for GxP environments?
Hanbon supplies IQ/OQ documentation packages, traceable calibration certificates for flow and pressure sensors, and a comprehensive risk assessment report aligned with ISO 14971.
What maintenance intervals are recommended for routine operation?
Daily solvent line purge, weekly column port inspection, and quarterly pump calibration—documented in the included Preventive Maintenance Manual (PMM) per ISO/IEC 17025 guidelines.
