Domel CENTRIC 400R Benchtop High-Speed Refrigerated Centrifuge
| Brand | Domel |
|---|---|
| Model | CENTRIC 400R |
| Type | Benchtop Refrigerated Centrifuge |
| Max Speed | 18,000 rpm |
| Max RCF | 31,151 × g |
| Max Capacity | 6 × 50 mL (angular rotor) / 4 × 200 mL (swing-out rotor) |
| Temperature Range | –9 °C to +40 °C |
| Acceleration/Deceleration Steps | 10 each |
| Timer Range | 10 s – 99 min 50 s or continuous |
| Cooling Time (20 °C → 4 °C) | ≤15 min |
| Noise Level | <58 dB(A) |
| Dimensions (H×W×D) | 350 × 650 × 545 mm |
| Net Weight | 79 kg |
| Power Supply | 230 V, 50/60 Hz |
| Motor | Brushless Induction Motor |
| Compliance | Designed for GLP-compliant laboratories |
Overview
The Domel CENTRIC 400R is a high-performance benchtop refrigerated centrifuge engineered for precision separation of biological macromolecules—including nucleic acids, proteins, subcellular organelles, and viral particles—in research, clinical diagnostics, and quality control laboratories. It operates on the principle of sedimentation under controlled gravitational force (RCF), where differential particle density, size, and shape enable reproducible stratification in solution. With a maximum rotational speed of 18,000 rpm and an RCF of 31,151 × g, the CENTRIC 400R delivers sufficient force for rapid pelleting of ribosomes, membrane vesicles, and extracellular vesicles without compromising sample integrity. Its integrated refrigeration system maintains precise temperature control between –9 °C and +40 °C, ensuring thermal stability during extended runs—critical for heat-sensitive enzymes, RNA preparations, and cryopreserved samples. The unit conforms to IEC 61010-1 for laboratory equipment safety and supports routine validation protocols required under GLP and ISO 13485 frameworks.
Key Features
- Brushless induction motor delivering high torque, low maintenance, and exceptional operational longevity
- Electric lid lock with dual interlock sensors ensuring mechanical and electronic safety compliance during operation
- Programmable acceleration and deceleration profiles (10-step settings), including soft-brake and no-brake modes for delicate pellet recovery
- LED display with intuitive interface supporting time, speed, RCF, temperature, and rotor ID recognition
- Quick-cool function achieving 4 °C from ambient (20 °C) in ≤15 minutes—optimized for pre-chill workflows prior to sample loading
- Low-noise design (<58 dB[A]) achieved through balanced rotor dynamics and vibration-dampening chassis architecture
- 10 programmable method storage slots enabling repeatable, operator-independent protocol execution
Sample Compatibility & Compliance
The CENTRIC 400R accommodates a broad spectrum of sample formats via 11 interchangeable rotors—including fixed-angle, swing-out, and microplate carriers—each validated for use across defined speed and load limits. Angular rotors support high-RCF applications such as plasmid DNA purification (e.g., RA 24/2: 18,000 rpm / 31,151 × g), while swing-out configurations (e.g., RS 4/200) facilitate high-volume cell harvesting at lower RCFs. All rotors are autoclavable or chemically sterilizable per manufacturer guidelines. The system complies with key regulatory expectations for analytical instrumentation: full traceability of run parameters (time, temperature, speed, RCF), audit-ready event logs, and secure user access levels—features aligned with FDA 21 CFR Part 11 readiness when integrated with compliant LIMS or ELN platforms.
Software & Data Management
While the CENTRIC 400R operates via embedded firmware without external PC dependency, its parameter logging architecture records timestamped metadata—including rotor type, setpoints, actual values, and error codes—for every run. These logs are exportable via USB interface in CSV format for integration into laboratory information management systems (LIMS) or electronic lab notebooks (ELN). Optional firmware upgrades may introduce enhanced data encryption and digital signature capabilities to meet evolving GxP documentation requirements. Routine calibration checks for speed accuracy (±10 rpm tolerance), temperature deviation (±1 °C at setpoint), and RCF consistency are supported using NIST-traceable reference standards.
Applications
- Isolation of mitochondria, lysosomes, and synaptic vesicles from tissue homogenates
- Purification of exosomes and microvesicles from serum, plasma, and conditioned media
- Clarification of bacterial lysates prior to chromatographic analysis
- Concentration of viral vectors in gene therapy process development
- Routine QC of monoclonal antibody formulations via aggregate sedimentation profiling
- High-throughput nucleic acid extraction in molecular diagnostics workflows
FAQ
What is the minimum and maximum operating temperature range?
The refrigeration system maintains temperatures from –9 °C to +40 °C, with ±1 °C stability at any setpoint within this range.
Can the CENTRIC 400R be used for ultracentrifugation applications?
No—it is classified as a high-speed centrifuge (not ultracentrifuge), with a maximum RCF of 31,151 × g; ultracentrifugation typically requires ≥100,000 × g.
Are rotor certifications included with purchase?
Yes—each rotor ships with a Certificate of Conformance detailing material composition, balance tolerance, and maximum rated speed/RCF per ISO 21501-4.
Does the system support remote monitoring or network connectivity?
Not natively; however, USB-based data export enables offline integration with centralized lab informatics infrastructure.
How often should preventive maintenance be performed?
Domel recommends biannual inspection of refrigerant integrity, motor brushless commutation diagnostics, and door latch calibration—per the maintenance schedule in the Operator’s Manual (Ref. DOC-C400R-OM-EN v3.2).
