Shiping SPH-111B & SPH-211B Standard Full-Temperature Orbital Shaking Incubator

Overview

The Shiping SPH-111B and SPH-211B are benchtop-to-floor-standing full-temperature orbital shaking incubators engineered for reproducible, long-duration cultivation and mixing under precisely controlled thermal and mechanical conditions. These units operate on the principle of orbital shaking—generating uniform circular motion (Φ25 mm orbit) to suspend cells, homogenize suspensions, enhance gas exchange, and promote consistent mass transfer across liquid-phase biological samples. Unlike reciprocating or vortex shakers, orbital motion minimizes shear stress while maximizing aeration efficiency—critical for aerobic microbial growth, mammalian cell expansion, and enzymatic reaction kinetics. The SPH-211B variant integrates a CFC-free R134a refrigeration system enabling operation down to 4°C, making it suitable for cold-adapted cultures, protein expression studies at reduced temperatures, and temperature-sensitive hybridization protocols. Both models employ PID-based microprocessor control with dual LCD display for simultaneous monitoring of setpoint and real-time values of temperature, speed, and elapsed time.

Key Features

• Single-axis balanced drive mechanism (Chinese patent) ensures low-noise, low-vibration operation with extended motor service life and minimal energy consumption.
• AC inverter motor delivers constant torque across the full 50–300 rpm range, with ±1 rpm speed accuracy and programmable acceleration profiles to prevent sample splashing during startup.
• Forced-air convection system with optimized airflow distribution achieves ±0.5°C temperature uniformity throughout the chamber (970 × 550 × 350 mm internal volume).
• Large tempered glass observation window with integrated LED lighting enables non-invasive visual monitoring without interrupting incubation conditions.
• Full-function safety architecture includes independent overtemperature cutoff, door-activated shutdown, leakage current protection, and automatic power recovery after grid interruption.
• Electrostatically coated steel frame and mirror-finish stainless-steel interior resist corrosion and facilitate decontamination—compliant with GLP laboratory hygiene standards.

Sample Compatibility & Compliance

The SPH-111B/211B accommodates standard culture vessels including Erlenmeyer flasks (up to 2000 mL × 10), test tubes, multi-well plates, and custom bioreactor vessels mounted on its 920 × 500 mm platform. Its wide temperature range (4°C–60°C for SPH-211B; ambient +5°C–60°C for SPH-111B) supports applications from psychrophilic bacterial propagation to thermophilic enzyme assays. The unit conforms to IEC 61010-1:2010 for laboratory electrical equipment safety and incorporates design elements aligned with ISO/IEC 17025 requirements for measurement traceability—including temperature and speed calibration functions accessible via front-panel interface. Optional UV germicidal lamps (254 nm) and sterile air inlet ports enable aseptic workflows compatible with ISO 14644-1 Class 5 cleanroom adjunct operations.

Software & Data Management

While the base configuration features embedded PID controller firmware with eight-segment programmable ramp-soak profiles, optional RS-485 communication enables integration into centralized lab management systems via Modbus RTU protocol. All operational parameters—including temperature setpoints, oscillation speed, timer status, and alarm logs—are retained in non-volatile memory during power loss. Real-time data logging (timestamped temperature/speed values) can be exported manually via USB or triggered remotely using TTL-level external triggers. Audit trail functionality satisfies basic FDA 21 CFR Part 11 requirements when paired with validated third-party LIMS software, supporting GMP-aligned documentation for QC microbiology and bioprocess development labs.

Applications

• Mammalian and insect cell line expansion in serum-containing or chemically defined media
• Aerobic cultivation of E. coli, Bacillus, and Pseudomonas strains for plasmid amplification and recombinant protein production
• Solubility screening and dissolution testing per USP <711> guidelines
• Antibody-antigen binding kinetics and ELISA plate pre-incubation
• Nucleic acid hybridization (Southern/Northern blotting), chromatin immunoprecipitation (ChIP) wash steps, and library preparation for NGS
• Environmental microbiology—enrichment of heterotrophic plate count (HPC) organisms and coliform detection
• Biodegradation studies requiring sustained agitation under controlled thermal regimes

FAQ

What is the difference between SPH-111B and SPH-211B?
The SPH-211B includes an integrated R134a refrigeration system enabling operation from 4°C to 60°C, whereas the SPH-111B operates from ambient +5°C to 60°C and lacks active cooling.
Can this shaker accommodate 5-L flasks?
No—the SPH-111B/211B platform is rated for maximum flask capacity of 2000 mL × 10. For 5-L vessel support, consider the SPH-111C/211C high-clearance variant.
Is the temperature calibration traceable to national standards?
Yes—built-in temperature sensor offset adjustment allows user calibration against NIST-traceable reference thermometers, with records stored in device memory.
Does the unit support continuous operation for 500 hours?
Yes—the timer is rated for uninterrupted 500-hour cycles; however, routine maintenance (e.g., bearing inspection, refrigerant pressure check) is recommended every 6 months for SPH-211B units operating below 15°C.
Are replacement parts and service manuals available internationally?
Shiping provides English-language technical documentation and maintains an authorized distributor network in North America, EU, and APAC regions for spare parts fulfillment and certified field service support.