Chu Ding Technology HSE-12D Rotary Valve-Based Solid Phase Extraction (SPE) Manifold

Overview

The Chu Ding Technology HSE-12D Rotary Valve-Based Solid Phase Extraction (SPE) Manifold is an engineered solution for high-integrity, reproducible sample cleanup in analytical laboratories. It operates on the principle of vacuum-assisted solid-phase extraction—where liquid samples are sequentially passed through sorbent-packed cartridges under controlled negative pressure to selectively retain target analytes or interferences based on physicochemical interactions (e.g., reversed-phase, ion exchange, or mixed-mode retention). Unlike conventional liquid–liquid extraction, the HSE-12D eliminates emulsion formation, minimizes solvent consumption, and enables precise control over flow rates and solvent strength gradients via independent per-channel rotary valves. Its circular architecture and modular valve system ensure uniform vacuum distribution across all 12 ports while preventing cross-contamination between samples—a critical requirement in trace-level analysis of complex matrices.

Key Features

• 12-position rotary valve manifold with individual on/off control per channel—enabling selective activation, sequential processing, and real-time flow adjustment without interrupting ongoing extractions.
• Integrated borosilicate glass vacuum chamber and thick-walled collection bottles—rated for sustained vacuum operation up to –80 kPa and resistant to thermal shock and organic solvents.
• Chemically inert fluid path: All wetted surfaces constructed from PTFE (valve bodies, seals, tubing), 316 stainless steel (manifold base, support frame), and high-purity glass—ensuring compatibility with aggressive solvents including acetonitrile, methanol, dichloromethane, and acidic/basic eluents.
• No-fog vacuum chamber design with dual-seal gasket interface—eliminates aerosol carryover and condensation-induced channel crosstalk during volatile solvent evaporation.
• Bottom-collection bottle configuration—facilitates simultaneous collection of both eluate fractions and waste effluent in separate, labeled vessels, supporting fractionated recovery and method validation.
• Modular scalability: Compatible with standard 1 mL, 3 mL, 6 mL, and 12 mL SPE cartridges from leading vendors (e.g., Waters, Agilent, Thermo Fisher, Phenomenex), as well as custom-packed columns.

Sample Compatibility & Compliance

The HSE-12D supports a broad range of aqueous and semi-aqueous sample types—including environmental water extracts, food homogenates, biological fluids (urine, plasma, serum), beverage filtrates, and soil leachates—provided they are pre-filtered (≤0.45 µm) and pH-adjusted where necessary to optimize cartridge retention. It meets fundamental design criteria referenced in ASTM D5907 (for waterborne contaminants), ISO 17025 (for laboratory competence), and USP (chromatographic system suitability prerequisites). When operated with audit-trail-capable vacuum controllers and documented SOPs, the system supports data integrity requirements aligned with FDA 21 CFR Part 11 for electronic records in regulated environments.

Software & Data Management

While the HSE-12D is a manually operated vacuum manifold, its mechanical architecture is fully compatible with third-party vacuum controllers featuring programmable pressure ramps, timed step protocols, and USB/RS-232 output (e.g., Vacuubrand RC 6, KNF N860.3FT.18). Integration with LIMS or ELN platforms is achieved via digital I/O triggers or CSV log export from connected controllers. Each valve position includes a tactile index mark and engraved port number for unambiguous sample tracking—supporting manual batch logs that satisfy GLP documentation standards for traceability, operator signature, date/time stamp, and cartridge lot recording.

Applications

• Environmental analysis: Extraction of organochlorine pesticides, PAHs, PCBs, and heavy metal chelates from surface water, wastewater, and sediment extracts.
• Food safety testing: Isolation of veterinary drug residues (e.g., sulfonamides, fluoroquinolones, β-agonists) from meat, milk, honey, and cereal matrices prior to LC-MS/MS quantification.
• Clinical and forensic toxicology: Cleanup of urine and blood samples for amphetamines, opioids, benzodiazepines, and synthetic cannabinoids.
• Pharmaceutical QC: Removal of excipients and degradation products from tablet dissolution media prior to HPLC assay.
• Beverage quality control: Clarification of fruit juices and alcoholic beverages for mycotoxin (e.g., ochratoxin A) and pesticide screening.

FAQ

Is the HSE-12D compatible with positive-pressure SPE systems?

No—the HSE-12D is designed exclusively for vacuum-driven SPE. Positive-pressure operation requires dedicated pneumatic manifolds with regulated gas supply and pressure sensors.
Can I use this manifold for offline fraction collection in multi-step elution protocols?

Yes. The bottom-collection bottle design allows direct placement of multiple vials beneath each port, enabling discrete fraction collection during gradient elution without cartridge removal.
What maintenance is required to sustain valve integrity and vacuum performance?

PTFE valve seats should be inspected quarterly for wear; replace if leakage exceeds 10% vacuum loss over 5 minutes at –70 kPa. Glass components require cleaning with acetone followed by deionized water rinse and air drying.
Does the system include vacuum pump and pressure gauge?

No—vacuum source and monitoring instrumentation are sold separately to allow user selection based on lab infrastructure and regulatory needs.
Are replacement valves and O-rings available as spare parts?

Yes. Chu Ding Technology supplies certified PTFE/stainless steel valve assemblies (Part No. HSE-V12-VALVE-KIT) and Viton-free fluorosilicone O-ring sets (Part No. HSE-OR-12) with full traceability documentation.