CBS-B Programmable Multi-Function Automatic Fraction Collector (Square-Design Platform)

Overview

The CBS-B Programmable Multi-Function Automatic Fraction Collector is an engineered liquid-handling platform designed for precise, reproducible fractionation in preparative chromatography workflows. Based on a robust square-frame mechanical architecture with programmable X–Y dual-axis positioning, it executes timed, volumetric (drop-count), and UV-signal-triggered (peak-based) collection with sub-millisecond timing resolution and millivolt-level analog signal responsiveness (0–1000 mV input range). Its core function is to interface seamlessly with preparative HPLC systems, medium-pressure liquid chromatography (MPLC), and gravity-fed or peristaltic column elution setups—enabling automated, unattended fraction isolation under controlled environmental conditions. Unlike legacy rotary or linear collectors, the CBS-B’s orthogonal motion system minimizes cross-contamination risk between adjacent tubes and supports high-density tube array configurations without compromising positional repeatability (< ±0.1 mm typical XY accuracy). It operates as a standalone unit or integrates via TTL/RS-232 into larger chromatographic ecosystems, satisfying fundamental requirements for method transfer, regulatory traceability, and scalable purification protocols.

Key Features

• Square-platform mechanical design with precision-guided X–Y stepper motor actuation for stable, low-vibration tube positioning
• Three independent collection modes: programmable time-based (1 s – 9999 min), drop-count-based (1–9999 drops), and analog peak-triggered (0–1000 mV DC input)
• High-contrast blue-backlit LCD display with intuitive Chinese-language menu navigation—no external PC required for basic operation
• Non-volatile memory retention: up to 10 user-defined collection programs preserved for ≥10 years after power loss
• Modular tube rack compatibility: accepts interchangeable trays supporting 16, 36, 100, or 169 tubes (e.g., optional SP-36L tray enables extended capacity without hardware modification)
• Integrated waste trough with overflow detection circuitry and dedicated drainage port—designed for continuous operation with organic and aqueous mobile phases
• Compact footprint (358 × 345 × 250 mm) optimized for benchtop integration in ISO Class 5–7 cleanrooms or GLP-compliant analytical labs

Sample Compatibility & Compliance

The CBS-B accommodates standard borosilicate glass and polypropylene test tubes (up to 12 mL volume per position), with chemical resistance validated for common HPLC solvents including acetonitrile, methanol, ethanol, chloroform, ethyl acetate, and aqueous buffers (pH 2–12). Its sealed electronics enclosure meets IP20 environmental rating for laboratory use. While not certified to IEC 61010-1 as a standalone safety-certified instrument, its operational parameters align with general laboratory equipment safety expectations under ISO/IEC 17025-accredited environments. Data integrity features—including persistent program storage and timestamped run logs—support audit readiness for GLP and GMP-aligned workflows where fraction traceability is critical (e.g., API purification, natural product isolation, or biomolecule characterization).

Software & Data Management

Operation is fully self-contained via the onboard microcontroller; no proprietary software installation is required. All parameters—including timing intervals, drop calibration factors, peak threshold offsets, and tray geometry mapping—are configured directly through the front-panel interface. Collection event logs (start time, mode used, tube index, elapsed duration, and trigger signal value) are stored internally and can be exported manually via RS-232 serial link to external terminals or LIMS-compatible databases. The device does not implement FDA 21 CFR Part 11 electronic signature functionality but provides sufficient metadata granularity for manual audit trail reconstruction in regulated settings when paired with validated chromatography data systems (CDS).

Applications

• Preparative HPLC fractionation of synthetic intermediates, pharmaceutical impurities, and reference standards
• Purification of peptides, oligonucleotides, and recombinant proteins using ion-exchange or size-exclusion chromatography
• Natural product isolation from plant extracts or microbial fermentation broths via MPLC or flash chromatography
• Method development studies requiring parallel fraction screening under variable gradient conditions
• Automated solvent removal pre-concentration workflows where timed collection precedes lyophilization or rotary evaporation
• Teaching laboratories performing undergraduate chromatography experiments with reproducible, hands-off fraction recovery

FAQ

Does the CBS-B support real-time UV absorbance integration with third-party detectors?
Yes—it accepts analog 0–1000 mV DC output signals from most commercial UV-Vis detectors (e.g., Waters 2489, Agilent G1315, Shimadzu SPD-20A) without signal conditioning.
Can I change tube rack configurations mid-run?
No—tube tray selection must be defined prior to program execution; however, the SP-36L and 100-tube trays are mechanically interchangeable between runs.
Is drop calibration required for volumetric collection?
Yes—users must perform initial drop-volume calibration using a calibrated cylinder and representative solvent viscosity to establish accurate mL/drop coefficients.
What is the maximum operating ambient temperature?
The unit is rated for continuous operation at 15–35 °C and relative humidity ≤80% non-condensing.
Does it include validation documentation?
Factory calibration certificates and IQ/OQ templates are available upon request for qualified customers engaged in regulated manufacturing or testing environments.