Corning 6575 Universal Clear PCR Sealing Tape
| Brand | Corning |
|---|---|
| Origin | USA |
| Manufacturer Type | Authorized Distributor |
| Product Origin | Imported |
| Model | 6575 Universal Clear PCR Sealing Tape |
| Pricing | Available Upon Request |
Overview
Corning® 6575 Universal Clear PCR Sealing Tape is a precision-engineered, pressure-sensitive optical sealing film designed specifically for real-time quantitative polymerase chain reaction (qPCR) applications. Unlike conventional adhesive tapes or mineral oil overlays, this sealing tape provides a chemically inert, optically transparent barrier that maintains full compatibility with fluorescence-based detection systems—including SYBR Green, TaqMan probes, and other common qPCR chemistries. Its formulation ensures minimal autofluorescence across UV–visible excitation ranges (300–700 nm), enabling high signal-to-noise ratio measurements without spectral interference. The tape adheres uniformly to standard 96-well and 384-well PCR microplates (including skirted, semi-skirted, and non-skirted formats), forming a hermetic seal that withstands thermal cycling from –70 °C to +100 °C—covering freeze-thaw storage, denaturation, annealing, and extension steps without delamination or edge lifting.
Key Features
- Optical Clarity & Low Autofluorescence: Engineered for maximal light transmission (>95% at 480–520 nm), supporting accurate fluorescence quantification in real-time PCR instruments from Bio-Rad, Thermo Fisher, Roche, and Agilent.
- Pressure-Sensitive Adhesion: Forms immediate, uniform bond upon light fingertip pressure—no heat activation or solvent required—ensuring reproducible sealing across all wells without bubble entrapment.
- Evaporation Control: Reduces sample loss to <0.5% per 40-cycle run when used with thermal cyclers equipped with heated lids; eliminates need for mineral oil overlay, minimizing contamination risk and simplifying workflow.
- Cryogenic & Thermal Stability: Maintains structural integrity and adhesive performance from –70 °C (long-term frozen storage) to +100 °C (denaturation phase), with no curling, cracking, or residue transfer.
- Chemical Resistance: Compatible with aqueous buffers (e.g., Tris-HCl, TE), common PCR master mixes, glycerol (≤20%), DMSO (≤10%), and ethanol—validated against ISO 10993-5 cytotoxicity requirements for labware contact.
Sample Compatibility & Compliance
The 6575 sealing tape is compatible with all standard PCR-grade polycarbonate and polypropylene microplates meeting ANSI/SBS dimensional specifications (ANSI SLAS 4–2004). It has been verified for use in GLP- and GMP-regulated environments where traceability and consistency are critical: lot-specific certificates of analysis (CoA) are available upon request, including peel adhesion (ASTM D3330), optical transmittance (ASTM D1003), and outgassing profiles (NASA ASTM E595). The product is not intended for sterilization via autoclaving or gamma irradiation; however, it is supplied in ISO Class 7 cleanroom-packaged rolls to ensure low particulate and endotoxin levels (<0.03 EU/mL by LAL assay).
Software & Data Management
While the sealing tape itself is a consumable and does not interface directly with instrument software, its consistent optical properties support reliable integration into automated qPCR data acquisition pipelines. When used with compliant microplates, it enables unambiguous Ct value determination and inter-run comparability—essential for FDA 21 CFR Part 11–aligned workflows requiring audit trails and electronic signature support. Corning provides digital lot documentation accessible via QR code on packaging, linking to CoA, stability data, and material safety information (SDS) in English, German, and Japanese.
Applications
- Real-time quantitative PCR (qPCR) and digital PCR (dPCR) assays requiring high-fidelity fluorescence detection
- Long-term cryopreservation of PCR-ready plates at –80 °C prior to thermal cycling
- High-content screening (HCS) workflows involving post-PCR immunostaining or enzymatic readouts
- Protein crystal imaging under inverted microscopes—its transparency and flatness minimize optical distortion and refractive index mismatch
- Multi-step nucleic acid amplification workflows integrating RT-qPCR, multiplex PCR, and melt curve analysis
FAQ
Is Corning 6575 tape compatible with 384-well low-profile plates?
Yes—it conforms reliably to shallow well geometries and exhibits no edge lift during ramp rates up to 5 °C/sec.
Can it be used for RNA stability studies involving repeated freeze-thaw cycles?
Yes—validated for ≥5 freeze-thaw cycles between –70 °C and 25 °C with no measurable increase in RNase activity or sample leakage.
Does the tape leave adhesive residue on plate surfaces after removal?
No—designed for clean removal using standard lab tweezers; residual tack is undetectable by FTIR spectroscopy (4000–400 cm⁻¹).
What is the shelf life under recommended storage conditions?
24 months when stored sealed at 15–25 °C and 30–60% RH; avoid direct UV exposure and ambient ozone sources.
