Agilent 1100 Series HPLC System (Refurbished)
| Brand | Agilent Technologies |
|---|---|
| Origin | USA |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | Agilent 1100 |
| Instrument Type | Fast Liquid Chromatograph |
| Flow Rate Range | 0.001–10.000 mL/min |
| Maximum Operating Pressure | 40 MPa |
| Sample Tray Capacity | 100 positions (1.5 mL vials) |
| Injection Volume Range | 1–50 µL |
| Column Oven Temperature Range | Ambient +10 °C to 60 °C |
| UV-Vis Detection Wavelength Range | 190–700 nm |
| Data Acquisition Rate | 100 Hz |
Overview
The Agilent 1100 Series HPLC System is a robust, modular high-performance liquid chromatography platform engineered for precision, reproducibility, and operational stability in regulated and research laboratory environments. Designed around a dual-piston reciprocating pump architecture with active solvent compressibility compensation, the system delivers exceptional flow accuracy (<0.1% RSD) and gradient fidelity across its full 0.001–10.000 mL/min range. Its 40 MPa maximum pressure rating supports both conventional 5 µm particle-packed columns and sub-3 µm superficially porous or fully porous media used in fast LC and UHPLC-compatible method development. The integrated diode array detector (DAD) provides real-time spectral acquisition from 190–700 nm at up to 100 Hz, enabling peak purity assessment, spectral library matching, and multi-wavelength quantitation without post-run reprocessing.
Key Features
- Modular architecture supporting configurable configurations: quaternary pump, autosampler, thermostatted column compartment, DAD, and optional fluorescence or refractive index detectors
- Autosampler with 100-position 1.5 mL vial capacity and programmable injection volumes from 1 to 50 µL with <1% RSD precision
- Column oven with precise temperature control from ambient +10 °C to 60 °C (±0.15 °C stability), critical for retention time reproducibility in method transfer
- Dual-beam optical design with deuterium and tungsten lamps ensuring stable baseline performance and extended lamp lifetime
- Low-dispersion flow cell (10 mm path length, 8 µL volume) optimized for high-resolution separations and low-dwell-volume gradient methods
- Comprehensive leak detection and pressure monitoring with automatic shutdown protocols to protect columns and system integrity
Sample Compatibility & Compliance
The Agilent 1100 accommodates standard 4.6 mm × 50–250 mm analytical columns and microbore formats down to 2.1 mm ID. It is routinely deployed in laboratories adhering to ISO/IEC 17025, USP , and ICH Q2(R2) validation guidelines. All hardware modules undergo full functional refurbishment—including pump seal replacement, detector lamp calibration, autosampler syringe cleaning and precision verification, and column oven thermal mapping—per Agilent-recommended service protocols. Systems are delivered with documented traceability to NIST-traceable standards and include a comprehensive compliance report covering pressure calibration, flow accuracy verification, UV absorbance linearity (per NIST SRM 930e), and dwell volume measurement.
Software & Data Management
The system operates under Agilent ChemStation Rev. A.10.02 or later, providing full GLP/GMP-compliant data acquisition, processing, and reporting. Audit trail functionality meets FDA 21 CFR Part 11 requirements, including electronic signatures, user access controls, and immutable record retention. Method validation templates support IQ/OQ/PQ documentation, while raw data files (.D format) are structured for long-term archival and third-party software interoperability (e.g., OpenLab CDS, Empower). Batch sequence management enables unattended overnight operation with automated recalibration and system suitability testing prior to sample injection.
Applications
This refurbished Agilent 1100 system is validated for routine use in pharmaceutical QC (assay, related substances, dissolution), environmental analysis (PAHs, pesticides, PFAS), food safety testing (mycotoxins, preservatives, vitamins), and academic research requiring high-fidelity LC-DAD quantitation. Its reliability in gradient elution of polar compounds (e.g., organic acids, nucleotides, small-molecule metabolites) and compatibility with reversed-phase, HILIC, and ion-pairing chemistries make it suitable for method development workflows where instrument familiarity and regulatory acceptance are essential. The 100-Hz acquisition rate ensures accurate integration of narrow peaks generated under fast-gradient conditions.
FAQ
Is this system qualified for GxP-regulated environments?
Yes—each unit undergoes full IQ/OQ qualification per Agilent’s recommended protocol, with documentation provided. Optional PQ support is available upon request.
What warranty and service support accompany the refurbished system?
A standard 12-month parts-and-labor warranty is included; extended service plans with on-site engineer response SLAs are available.
Can the system be upgraded to support modern connectivity or additional detectors?
Yes—the modular design permits integration of newer Agilent modules (e.g., 1260 Infinity II DAD, ALS, or VWD) via compatible interface kits and firmware updates.
Are original Agilent consumables and spare parts still available?
All core consumables—including pump seals, injector rotors, DAD flow cells, and column ovens—are actively supported by Agilent and global distributors through 2027.
Does the system include validated methods or application notes?
A curated library of Agilent-certified application notes (e.g., USP monographs, EPA methods, AOAC guidelines) is supplied with the system, along with method transfer checklists and system suitability templates.
