Agilent 1100 Series Autosampler (Refurbished)
| Brand | Agilent Technologies |
|---|---|
| Origin | USA |
| Model | 1100 |
| Instrument Type | HPLC Autosampler |
| Sample Capacity | Up to 100 × 2 mL vials (standard tray) |
| Injection Volume Range | 0.1–100 µL (standard) |
| Carryover | <0.05% with needle wash cycle |
| Precision | RSD < 0.3% (typical, 10 µL injection) |
| Temperature Control Range | 4–40 °C (1 °C increment, optional thermostatted models) |
| Maximum Operating Pressure | 400 bar |
| Compatible Formats | 96-well & 384-well microplates, 2 mL vials, 6 mL vials, custom tubes |
| Compliance | Designed for GLP/GMP environments |
Overview
The Agilent 1100 Series Autosampler is a precision-engineered sample introduction module designed for integration with Agilent’s 1100 Series HPLC systems. Based on robust syringe-driven positive displacement technology, it delivers high reproducibility and minimal carryover across diverse analytical workflows. Unlike pressure-driven or loop-based injectors, the 1100 autosampler employs a sealed, air-free fluid path with active needle washing—ensuring consistent injection volume accuracy and long-term stability in regulated environments. Its modular architecture supports both routine QC analysis and method development in pharmaceutical, academic, and contract research laboratories. As a refurbished unit, this instrument undergoes full functional validation—including leak testing, injection precision verification, temperature calibration (where applicable), and firmware integrity checks—prior to shipment.
Key Features
- Syringe-based positive displacement injection mechanism for precise, pulse-free sample delivery
- Configurable sample trays: standard 100-position 2-mL vial rack; optional 40-position 2-mL or 15-position 6-mL configurations
- Microplate compatibility: supports dual 96-well or 384-well plate carriers (G1329B microplate sampler option)
- Low-volume injection capability: standard range 0.1–100 µL; optional ultra-low volume kits extend down to 0.01 µL
- Integrated needle wash station with programmable solvent selection and dwell time
- Thermostatted versions available (G1329A): temperature-controlled sample compartment (4–40 °C, ±0.5 °C stability)
- High-pressure tolerance: rated for continuous operation up to 400 bar, compatible with UHPLC-capable columns when paired with appropriate pump modules
- Modular design enables field-upgradeable options including stackable trays, barcode readers, and external dilution modules
Sample Compatibility & Compliance
The Agilent 1100 autosampler accommodates standard ANSI/SLAS-compliant vials (12×32 mm), crimp-top and snap-cap formats, as well as 96- and 384-well microplates (SBS footprint). Sample loops, capillary connections, and needle assemblies are constructed from inert materials—including PEEK, stainless steel, and sapphire—to minimize adsorption and degradation of sensitive analytes (e.g., peptides, oligonucleotides, labile metabolites). The system meets mechanical and electrical safety requirements per IEC 61010-1 and is fully compatible with Agilent ChemStation and OpenLab CDS software platforms. When deployed in validated environments, it supports ALCOA+ data integrity principles and can be configured to generate electronic records compliant with FDA 21 CFR Part 11, EU Annex 11, and ISO/IEC 17025 documentation standards.
Software & Data Management
Control and method programming are executed via Agilent ChemStation (Rev. A.10.x or later) or OpenLab CDS (v2.5+), enabling full sequence definition, injection parameter scripting (e.g., partial loop fill, needle wash cycles, post-injection flush), and real-time status monitoring. Audit trail functionality logs all user actions—including method edits, sequence changes, and calibration events—with immutable timestamps and operator identification. Data export conforms to ASTM E1957 and AIA/CDIF chromatographic data interchange standards. Raw signal files (.D directories) retain full metadata: injection time, vial position, wash cycle count, pressure trace snippets (if pump-integrated), and hardware error flags.
Applications
- Pharmaceutical stability-indicating assays requiring low carryover and high inter-day repeatability
- High-throughput screening (HTS) of compound libraries using 384-well plate formats
- Regulatory bioanalysis (PK/PD studies) under GLP conditions with full traceability
- Method transfer between R&D and QC labs due to standardized hardware interface and method portability
- Environmental residue analysis where matrix complexity demands rigorous needle cleaning protocols
- Academic teaching labs requiring rugged, serviceable instrumentation with documented maintenance history
FAQ
Is this unit factory-refurbished or third-party refurbished?
This unit has undergone certified refurbishment by an Agilent-authorized service partner, including replacement of wear-prone components (syringe seals, rotor seals, needle, wash port valves), full hydraulic pressure testing, and firmware update to the latest stable release.
Does it include a warranty?
Yes—a 12-month limited hardware warranty is included, covering parts and labor for defects in materials or workmanship under normal laboratory use.
Can it be integrated with non-Agilent HPLC systems?
Mechanical and electrical interfaces follow standard Agilent GPIB and RS-232 protocols; analog trigger outputs and TTL-compatible start signals allow integration with select third-party pumps and detectors—but full sequence synchronization requires Agilent CDS software.
What documentation accompanies the unit?
Each shipment includes a Certificate of Refurbishment, calibration report (injection volume accuracy, carryover test results, temperature uniformity map), service history log, and original Agilent operational manuals in English.
Is remote diagnostic support available?
Yes—Agilent Remote Assistance (via secure VPN) is supported for troubleshooting, provided the host PC meets minimum network security requirements and runs current OpenLab CDS client software.
