Qiangle LSHZ-300 Cryogenic Water Bath Shaker

Overview

The Qiangle LSHZ-300 Cryogenic Water Bath Shaker is an engineered platform for precise temperature-controlled agitation of liquid samples in life science laboratories. It operates on the principle of orbital shaking within a thermally regulated water bath, enabling simultaneous control of both thermal environment and mechanical mixing intensity. Unlike air-cooled shakers, the LSHZ-300 integrates a dedicated refrigeration circuit with high-efficiency compressor cooling (280 W), allowing stable sub-ambient operation down to −10 °C — critical for preserving enzyme activity, preventing thermal denaturation of nucleic acids, or maintaining viability of sensitive microbial cultures during extended incubation. Its stainless-steel water bath chamber accommodates up to fourteen 250 mL flasks or culture vessels, supporting scalable parallel processing while maintaining uniform temperature distribution (±0.3 °C at 25 °C, ±0.5 °C across full range) and consistent oscillation dynamics across all positions.

Key Features

• Refrigerated temperature control from −10 °C to +99 °C, achieved via dual-mode thermal regulation (compressor-assisted cooling + PID-controlled heating)
• Adjustable orbital oscillation frequency from 40 to 280 rpm, with digital feedback loop ensuring speed stability within ±1 rpm under load
• Robust 1500 W power architecture with independent compressor circuitry, optimized for continuous-duty operation in 24/7 laboratory environments
• Stainless-steel water bath tank (depth ≥120 mm) designed for compatibility with standard Erlenmeyer flasks, test tubes, and multi-well plates using optional clamping accessories
• Microprocessor-based controller with real-time display of setpoint and actual values for both temperature and rpm, plus programmable ramping profiles
• CE-compliant safety design per IEC 61010-1, including overtemperature cutoff, motor overload protection, and leak detection circuitry

Sample Compatibility & Compliance

The LSHZ-300 supports aqueous and buffered solutions across common life science applications — including bacterial and yeast fermentation, protein solubilization, cell lysis buffer equilibration, and immunoassay plate washing protocols. Its temperature uniformity and low-vibration orbital motion minimize shear stress on fragile cells or aggregated biomolecules. The unit meets essential electrical and mechanical safety standards required for installation in ISO 17025-accredited testing labs and GxP-regulated environments. While not inherently 21 CFR Part 11 compliant, its digital interface supports audit-trail-capable data logging when integrated with validated third-party software systems adhering to ALCOA+ principles. Documentation includes factory calibration certificates traceable to NIM (National Institute of Metrology, China), and optional IQ/OQ documentation packages are available upon request.

Software & Data Management

The onboard controller stores up to 10 user-defined programs with independent temperature/rpm/time parameters. Data output is accessible via RS-232 serial port (standard) or optional USB-to-serial adapter for direct transfer to PC-based lab information management systems (LIMS). Exported logs include timestamped entries for setpoint, measured temperature, actual rpm, and system status flags (e.g., compressor active, alarm triggered). When used with compliant external software, the device supports electronic record retention aligned with GLP and GMP documentation requirements, including operator ID assignment and change history tracking.

Applications

• Molecular biology: Plasmid amplification in LB medium at controlled 37 °C with gentle agitation to prevent foam formation
• Pharmaceutical QC: Dissolution testing of solid dosage forms under USP conditions requiring precise temperature maintenance
• Bioprocessing: Seed train expansion of mammalian cell lines at hypothermic conditions (e.g., 33 °C) to extend viability and reduce metabolic byproduct accumulation
• Environmental microbiology: Enumeration of psychrotrophic bacteria in food samples via enrichment at 7 °C with continuous shaking
• Proteomics: In-solution digestion of denatured proteins at 37 °C with constant mixing to enhance trypsin accessibility

FAQ

Does the LSHZ-300 require external coolant or antifreeze additives to reach −10 °C?
Yes. Operation below 0 °C requires addition of a non-toxic, water-miscible cryogenic fluid (e.g., ethylene glycol/water mixture) to the bath reservoir. Pure water freezes at 0 °C and cannot sustain sub-zero temperatures.
Is the oscillation amplitude adjustable?
No. The LSHZ-300 employs a fixed 25 mm orbital diameter optimized for efficient mixing without splashing or vortex formation in standard laboratory vessels.
Can this unit be stacked or installed inside a cold room?
It is not rated for cold room installation. Ambient operating conditions must remain between 15 °C and 30 °C with relative humidity ≤80% non-condensing to ensure reliable compressor performance and condensate management.
What maintenance intervals are recommended?
Compressor oil level and refrigerant pressure should be verified annually by qualified service personnel. Bath water should be replaced weekly or after each use involving biological contaminants, and the interior wiped with 70% ethanol between runs.
Is there a built-in timer function?
No. The unit operates in continuous mode only; external programmable timers or building automation systems must be used for timed shutdown sequences.