Hesix HR26M Vertical High-Speed Refrigerated Titanium Alloy Continuous-Flow Centrifuge

Overview

The Hesix HR26M is a vertically oriented, high-speed refrigerated continuous-flow centrifuge engineered for large-volume bioprocessing applications requiring sustained separation performance under precisely controlled thermal conditions. It operates on the principle of sedimentation-based continuous separation: sample feed enters the titanium alloy continuous-flow rotor at a defined volumetric rate while rotating at up to 25,000 rpm; particles—ranging from whole cells and subcellular organelles to virus particles and protein aggregates—are pelleted against the rotor wall by centrifugal forces exceeding 58,000 × g, while clarified supernatant exits continuously via an integrated outlet channel. Unlike batch-mode ultracentrifuges, the HR26M enables uninterrupted processing of fermentation broths, cell lysates, or clarified harvest streams—critical for scalable downstream operations in vaccine manufacturing, therapeutic enzyme purification, and industrial biotechnology. Its vertical architecture optimizes floor space utilization in GMP-compliant cleanrooms and central processing labs, while its titanium rotor construction ensures exceptional corrosion resistance and mechanical integrity during prolonged operation with saline, detergent-containing, or mildly acidic/alkaline biological fluids.

Key Features

• High-strength forged titanium alloy continuous-flow rotor (3 L working volume), certified for ≥10,000 operational hours at rated speed and temperature
• Refrigeration system using environmentally compliant R404a refrigerant and hermetically sealed non-CFC compressor, delivering stable thermal control from –20 °C to +40 °C with ±1 °C accuracy
• Pre-cooling and standby cooling functions enable rapid thermal equilibration prior to run initiation and maintenance of setpoint during idle periods
• TFT-LCD capacitive touchscreen interface with real-time dual-parameter display (set vs. actual speed, temperature, time, RCF); supports English and Chinese language modes
• Triple-layer safety architecture: austenitic 304 stainless steel centrifuge chamber, powder-coated steel housing, reinforced front panel, and mechanically interlocked lid with auto-engaging latch
• AC frequency-controlled motor with microprocessor-based speed regulation (±50 rpm precision) and dynamic imbalance compensation
• 16 programmable protocols stored internally, supporting repeatable method execution across shifts and operators

Sample Compatibility & Compliance

The HR26M accommodates a broad spectrum of biological and environmental samples, including microbial fermentation broths, mammalian and insect cell culture harvests, tissue homogenates, plasma-derived fractions, seawater/estuarine suspensions, and nanoparticle dispersions. Its continuous-flow configuration is validated for use in human vaccine production workflows—including inactivated whole-virus vaccines (e.g., avian influenza, foot-and-mouth disease, porcine reproductive and respiratory syndrome)—where regulatory adherence to process consistency and contamination control is mandatory. The instrument holds CFDA registration and is manufactured under ISO 13485:2016-certified quality management systems, ensuring traceability of materials, calibration records, and design history files. While not inherently 21 CFR Part 11–compliant out-of-the-box, its audit-ready data logging (timestamped speed, temperature, duration, rotor ID) supports integration into validated electronic lab notebook (ELN) or MES environments meeting GLP/GMP documentation requirements.

Software & Data Management

The embedded firmware logs all critical run parameters—including start/stop timestamps, rotor serial number, actual vs. target RCF, cumulative runtime, and thermal deviation history—with automatic timestamping and non-volatile storage. Export is supported via USB port in CSV format for offline analysis in Excel or statistical software (e.g., JMP, Minitab). Optional RS-232 or Ethernet connectivity modules (sold separately) allow remote monitoring and centralized fleet management in multi-instrument laboratories. All user actions—including parameter edits, protocol recalls, and door opening events—are recorded in an immutable event log accessible via service mode, fulfilling basic ALCOA+ data integrity principles (Attributable, Legible, Contemporaneous, Original, Accurate).

Applications

• Continuous clarification of >100 L/h bacterial or yeast fermentation broths for recombinant protein recovery
• Harvest and concentration of mammalian suspension cells (CHO, HEK293) without shear-induced lysis
• Downstream processing of viral vectors and bacteriophage preparations in gene therapy development
• Isolation of extracellular vesicles (exosomes) and ribonucleoprotein complexes from biofluids
• Concentration of phytoplankton, zooplankton, or suspended solids from marine/estuarine water samples
• Separation of immune globulins (IgG, IgM) from plasma-derived intermediates
• Recovery of intracellular enzymes (e.g., uricase, asparaginase) post-cell disruption
• Preparative-scale isolation of inclusion bodies or membrane protein aggregates

FAQ

What is the maximum continuous throughput capacity of the HR26M?

The titanium continuous-flow rotor supports a nominal processing rate of 3 L/h at optimal rotor loading and flow dynamics; actual throughput depends on particle size distribution, viscosity, and desired separation resolution.

Can the HR26M be used for sterile processing?

Yes—the rotor and fluid path components are autoclavable (121 °C, 20 min), and the sealed refrigeration system prevents condensate ingress into the chamber; full aseptic validation requires additional facility-level controls (e.g., HEPA-filtered inlet gas, closed-loop fluid transfer).

Is rotor balancing required before each run?

No—continuous-flow operation eliminates the need for manual balancing; however, rotor integrity checks (crack inspection, surface finish assessment) must be performed per scheduled maintenance intervals.

Does the HR26M support FDA 21 CFR Part 11 compliance?

The base unit provides audit-trail-capable data logging but requires third-party software integration (e.g., LabVantage, Thermo Fisher SampleManager) to achieve full electronic signature, role-based access, and electronic record retention functionality.

What certifications apply to the HR26M for international export?

It carries CE marking (per Machinery Directive 2006/42/EC and EMC Directive 2014/30/EU), RoHS 2011/65/EU compliance, and ISO 13485:2016 certification—serving as foundational documentation for regulatory submissions in ASEAN, GCC, and Latin American markets.