Revvity Zephyr G3 NGS Liquid Handling Workstation
| Brand | Revvity |
|---|---|
| Origin | USA |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Category | Imported Instrument |
| Model | Zephyr G3 NGS |
| Plate Capacity | 96-well format |
| Pipetting Range | 1–200 µL |
| Sample Throughput | 8–48 NGS libraries per run (including exome libraries) |
| Dimensions (H × W × D) | 648 mm × 749 mm × 559 mm (25.5" × 29.5" × 22") |
Overview
The Revvity Zephyr G3 NGS Liquid Handling Workstation is a benchtop, fully integrated automation platform engineered specifically for reproducible, walk-away execution of next-generation sequencing (NGS) library preparation workflows. It operates on the principle of positive-displacement liquid handling with air-gap and tip-touch optimized dispensing—ensuring high accuracy and low carryover across viscous, volatile, or low-volume reagents common in adapter ligation, fragmentation, end-repair, A-tailing, indexing PCR, and cleanup steps. Designed to enforce physical separation between pre- and post-PCR workspaces—a critical requirement for contamination control in molecular diagnostics and clinical genomics—the Zephyr G3 conforms to ISO 15189 and CLIA laboratory design best practices. Its compact footprint integrates seamlessly into regulated environments while supporting both research-grade discovery pipelines and GLP-compliant assay development.
Key Features
- Pre-validated, vendor-agnostic protocol library covering Illumina, Thermo Fisher Ion Torrent, PacBio, and Oxford Nanopore-compatible kits—including exome capture, targeted panel, whole-genome, and RNA-seq workflows
- Flexible throughput architecture: supports 8–48-library parallel processing per run with dynamic deck reconfiguration; full 96-well plate capacity for magnetic bead-based size selection and post-PCR purification
- High-precision 1–200 µL pipetting range with dual-channel or 8-channel disposable tip options, calibrated per ISO 8655 and traceable to NIST standards
- Integrated thermal modules (heating/cooling blocks and thermocyclers) and magnetic separation racks enable end-to-end automation without external instrumentation
- Graphical drag-and-drop method editor with real-time simulation, error logging, and audit-trail-enabled method versioning compliant with FDA 21 CFR Part 11 requirements
- Modular deck layout adheres to ANSI/SLAS microplate footprint standards, facilitating cross-platform method transfer and validation across laboratories
Sample Compatibility & Compliance
The Zephyr G3 accommodates standard SBS-format microplates (96-well, 384-well), tube racks (1.5 mL, 2.0 mL), and custom labware via user-defined coordinate mapping. It handles diverse sample types including fragmented genomic DNA, FFPE-derived nucleic acids, cfDNA, and cDNA—compatible with enzymatic and mechanical shearing inputs. All liquid handling operations meet ISO 5725 precision criteria for repeatability (CV < 3% at ≥10 µL) and trueness (recovery 95–105%). The system is validated for use in laboratories operating under CAP, CLIA, and EU IVDR frameworks, with documentation packages available for IQ/OQ/PQ execution and regulatory submissions.
Software & Data Management
Powered by Revvity’s Zephyr Control Software v4.x, the workstation provides role-based access control, electronic signature support, and automated generation of raw data logs (CSV, XML) synchronized with LIMS via HL7 or RESTful API. All method changes, run logs, and calibration records are timestamped and digitally signed, fulfilling ALCOA+ (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) data integrity principles. Audit trails are immutable and exportable for internal review or regulatory inspection. Optional integration with Revvity’s AssayMAP® cloud analytics enables QC trend analysis and predictive maintenance alerts.
Applications
- Automated NGS library construction for oncology panels, inherited disease screening, and infectious agent detection
- High-fidelity post-PCR cleanup and size selection using SPRI-based magnetic beads (e.g., AMPure XP, Agencourt RNAClean XP)
- Exome enrichment workflows requiring precise hybridization capture timing and stringent wash stringency control
- Scalable transition from manual to automated library prep during assay validation or clinical test implementation
- Multi-omics sample prep harmonization—coordinating DNA, RNA, and methylation library generation on a single platform
FAQ
Does the Zephyr G3 support third-party reagents and consumables?
Yes—it accepts all ANSI/SLAS-compliant tips, plates, and tubes; method templates are adaptable to non-Revvity kits via volume and timing parameter adjustment.
Can it be integrated into an existing LIMS or ELN environment?
Yes—through configurable HL7 v2.x messaging, ODBC database connectors, or secure HTTPS-based webhooks for bidirectional data exchange.
Is remote monitoring and troubleshooting supported?
Yes—via Revvity Remote Connect, which enables encrypted screen sharing, log file retrieval, and firmware update deployment without on-site service visits.
What regulatory documentation is provided for GxP environments?
A complete validation toolkit is included: URS, FRS, DQ/IQ/OQ/PQ protocols, calibration certificates, and 21 CFR Part 11 compliance evidence pack.
How frequently does the system require preventive maintenance?
Recommended every 6 months or after 1,000 operational hours, with automated PM scheduling and parts-lifetime tracking embedded in the software dashboard.
