Hanil J-075(A/T) Tubular Continuous-Flow Centrifuge

Overview

The Hanil J-075(A/T) Tubular Continuous-Flow Centrifuge is an industrial-grade, high-speed separation system engineered for continuous processing of biological, microbial, and botanical suspensions. Based on the hydrodynamic principle of tubular bowl centrifugation—where sample flow is axial while sedimentation occurs radially under sustained high gravitational fields—the J-075(A/T) achieves efficient solid-liquid phase separation without batch interruption. Its 2.2 L rotor chamber operates at up to 19,000 rpm, generating a maximum relative centrifugal force (RCF) of 15,800 × g, enabling rapid pelleting of submicron particles, whole cells, organelles, and precipitated biomolecules. Designed for integration into pilot-scale or GMP-aligned downstream processes, the unit delivers a nominal throughput of 500 L/h with consistent residence time control—critical for maintaining product integrity in sensitive bioprocessing applications such as monoclonal antibody harvest, yeast lysate clarification, or algal biomass concentration.

Key Features

• Continuous-flow architecture eliminates manual batch loading/unloading, reducing operator intervention and contamination risk.
• Quadruple mechanical door interlock system complies with IEC 61010-1 safety requirements and prevents operation with the front access door open.
• Tubular rotor geometry ensures laminar flow profiles and uniform shear distribution—minimizing cellular lysis or protein denaturation during high-throughput processing.
• Corrosion-resistant stainless-steel housing (AISI 316L grade components in wetted zones) withstands repeated exposure to acidic, alkaline, or saline process streams.
• Integrated vibration-damping base and precision-balanced spindle assembly limit operational noise to ≤70 dB(A), meeting ISO 1996-2 ambient noise guidelines for laboratory and cleanroom environments.
• PTFE-coated sample collection surfaces facilitate non-adherent recovery of fragile pellets—ideal for subsequent lyophilization or formulation steps.
• Digital LCD interface displays real-time RPM, RCF, elapsed time, and system status; all parameters are adjustable via tactile membrane keypad with password-protected configuration modes.
• Front-access door design enables rapid rotor inspection, cleaning validation, and maintenance without disassembling peripheral plumbing or drive components.

Sample Compatibility & Compliance

The J-075(A/T) accommodates a broad range of suspension types including bacterial cultures (E. coli, Bacillus spp.), mammalian cell harvests (CHO, HEK293), microalgae (Chlorella, Spirulina), plant extracts, and colloidal nanoparticle dispersions. It supports both aqueous and low-viscosity organic-aqueous mixtures (e.g., ethanol/water extracts) within pH 3–11 and temperature ranges of 4–40 °C (ambient-cooled operation). The system meets essential regulatory prerequisites for analytical and manufacturing use: compliance with ISO 22870 (in vitro diagnostic medical devices), adherence to ISO 13485 quality management principles for device manufacturing, and compatibility with GLP/GMP documentation workflows—including audit-trail-ready parameter logging when interfaced with validated external data acquisition systems.

Software & Data Management

While the J-075(A/T) operates as a standalone instrument with embedded firmware, its digital control module provides RS-232 and optional Ethernet (Modbus TCP) connectivity for integration into centralized lab automation platforms. All setpoints (speed, run time, acceleration/deceleration ramp rates) and real-time operational data (actual RPM, RCF, motor current, thermal load) can be exported in CSV format for traceability. When deployed in regulated environments, users may configure external SCADA or LIMS interfaces to satisfy FDA 21 CFR Part 11 requirements—including electronic signatures, user-level access control, and immutable event logs—for full data integrity assurance.

Applications

• Primary harvest of recombinant proteins from microbial fermentation broths.
• Clarification of clarified lysates prior to chromatographic purification.
• Concentration and washing of vaccine antigens (e.g., viral vectors, VLPs).
• Recovery of intracellular enzymes from thermophilic bacteria.
• Downstream processing of CRISPR-Cas ribonucleoprotein complexes.
• Preparative isolation of exosomes from conditioned cell culture media.
• Continuous removal of cell debris in perfusion bioreactor effluent streams.

FAQ

What is the recommended maintenance interval for the J-075(A/T)?
Routine inspection of rotor integrity, door seal condition, and drive belt tension is advised every 500 operating hours; full calibration verification (speed/RCF accuracy, timer drift) should occur annually per ISO/IEC 17025 guidelines.
Can the J-075(A/T) be validated for GMP production use?
Yes—the unit’s mechanical design, material certifications (EN 10204 3.1), and repeatable performance metrics support IQ/OQ/PQ protocol development; Hanil provides Factory Acceptance Test (FAT) documentation upon request.
Is rotor balancing required before each run?
No—the tubular rotor is dynamically balanced at the factory and sealed; imbalance-induced vibration is mitigated by the integrated active damping system and does not require user recalibration.
Does the system support sterile processing?
While not autoclavable, the rotor chamber and fluid pathways are CIP-compatible using validated sodium hydroxide or hydrogen peroxide solutions; full-system SIP requires integration with external steam generators.
What safety certifications does the J-075(A/T) hold?
It carries CE marking per Machinery Directive 2006/42/EC and EMC Directive 2014/30/EU; electrical safety conforms to UL 61010-1 and IEC 61010-1:2010 third edition standards.