Hanuo HN-6020SE Microbial Sample Preprocessing Instrument

Overview

The Hanuo HN-6020SE Microbial Sample Preprocessing Instrument is a benchtop thermal oscillation platform engineered for precise, reproducible conditioning of microbial suspensions prior to downstream analytical workflows—including flow cytometry, colony-forming unit (CFU) enumeration, viability staining, and nucleic acid extraction. It operates on the principle of controlled convective heating combined with low-frequency, high-amplitude horizontal shaking to ensure uniform thermal exposure and suspension homogeneity without shear-induced cell lysis or clumping. Unlike generic incubators or shakers, the HN-6020SE integrates real-time thermal regulation with programmable agitation—enabling standardized re-suspension, dispersion, and temperature equilibration of bacterial cultures, yeast pellets, biofilm disaggregates, and environmental isolates. Its compact footprint and sealed chamber design minimize ambient interference, making it suitable for biosafety level 1 (BSL-1) laboratories and cleanroom-adjacent workflows.

Key Features

• Brushless DC motor drive ensures silent operation (<45 dB(A)), electromagnetic compatibility with sensitive instrumentation (e.g., flow cytometers), and zero maintenance over extended service life.
• Dual-layer overtemperature protection: hardware-based thermal cutoff (independent of control board) and software-monitored PID loop with automatic shutdown at ≥65°C.
• Lid-open safety interlock halts oscillation and heating instantly upon chamber access—preventing accidental thermal shock or mechanical injury during sample loading/unloading.
• Power-loss recovery retains all active parameters and resumes operation from the exact timepoint and setpoint after grid interruption—critical for unattended overnight protocols.
• Onboard temperature calibration function allows user-initiated offset correction against traceable NIST-certified reference thermometers, ensuring long-term metrological consistency.
• Intuitive 3.5-inch TFT LCD interface displays real-time temperature, speed, elapsed time, remaining time, and system status—supporting simultaneous monitoring of up to four independent parameters without menu nesting.

Sample Compatibility & Compliance

The HN-6020SE accommodates standard microbiological vessels including 15 mL and 50 mL conical centrifuge tubes, multi-well plates (up to 96-well format with optional adapter), Eppendorf tubes (1.5–2.0 mL), and custom glass vials (diameter ≤60 mm). Its 230 × 230 mm platform and 182 mm internal height permit vertical stacking of tube racks while maintaining stable oscillation dynamics. The instrument is validated for use with Gram-positive and Gram-negative bacteria (e.g., E. coli, S. aureus, B. subtilis), yeasts (S. cerevisiae, C. albicans), and spore suspensions. It complies with ISO 15195:2018 (reference measurement systems), CLSI EP26-A (preanalytical phase validation), and supports adherence to GLP documentation requirements through timestamped operational logs (accessible via USB export). While not FDA-cleared, its design aligns with 21 CFR Part 11 data integrity principles when paired with validated LIMS integration.

Software & Data Management

The embedded firmware records full-cycle metadata—including start/stop timestamps, target vs. actual temperature profiles, RPM history, and fault codes—for each run. Logs are stored in CSV format on removable microSD card (included) and can be exported for audit trails, SOP verification, or method transfer documentation. No proprietary software is required; raw files are human-readable and compatible with Excel, Python pandas, or laboratory informatics platforms. Remote parameter adjustment is not supported—intentionally limiting attack surface per cybersecurity best practices for research infrastructure.

Applications

• Standardized resuspension of cryopreserved bacterial stocks prior to flow cytometric viability assessment using SYTO 9/PI dual staining.
• Controlled dispersion of Pseudomonas aeruginosa biofilms for antibiotic susceptibility testing under physiologically relevant shear conditions.
• Thermal activation of heat-shock competent cells prior to plasmid transformation protocols.
• Equilibration of clinical sputum or stool suspensions to 37°C before automated CFU plating or MALDI-TOF MS preparation.
• Pre-analytical conditioning of environmental water samples containing mixed microbial consortia for subsequent qPCR or metagenomic library prep.

FAQ

Does the HN-6020SE support programmable multi-step temperature ramps?
No. It operates in single-setpoint mode only—optimized for isothermal incubation with agitation. For ramped protocols, external controller integration is required.
Can the instrument be used inside a CO₂ incubator?
No. Its rated operating ambient is 15–30°C and 30–80% RH non-condensing. Enclosure within humidified CO₂ environments may compromise thermal sensor accuracy and electrical insulation.
Is the oscillation motion truly horizontal and sinusoidal?
Yes. The eccentric cam-driven mechanism produces pure lateral displacement (20 mm peak-to-peak) with minimal vertical coupling (<0.3 mm), verified by laser vibrometry per ISO 20486:2018.
What validation documentation is provided?
Factory-issued IQ/OQ protocol templates, temperature mapping report (9-point chamber validation at 37°C and 60°C), and motor torque certification are included with shipment.
How often does the built-in temperature calibration require verification?
Annual verification against an accredited reference thermometer is recommended; more frequent checks are advised if used in regulated GxP environments or following equipment relocation.