Hanuo HN70-2A Microplate Incubator Shaker

Overview

The Hanuo HN70-2A Microplate Incubator Shaker is an integrated benchtop instrument engineered for precise temperature-controlled orbital shaking of standard microplates (e.g., 96-well and 384-well formats). It combines PID-regulated conductive heating with a brushless DC motor-driven horizontal orbital motion system to support applications requiring simultaneous thermal incubation and homogeneous mixing—such as enzyme-linked immunosorbent assay (ELISA) development, cell-based assay optimization, antigen-antibody binding kinetics, and microbial suspension culture. Unlike generic shakers or standalone incubators, this device features dual-sided heating plates that ensure uniform thermal distribution across the entire plate surface, minimizing well-to-well temperature gradients—a critical factor in reproducible biological assays.

Key Features

• Intuitive LCD interface displaying real-time values for temperature, time remaining, and oscillation speed—enabling immediate verification of operational parameters during assay runs.
• Touch-sensitive control panel with ergonomic layout and seamless stainless-steel housing designed for compatibility with biosafety cabinets and cleanroom environments; surface geometry facilitates rapid decontamination and routine maintenance.
• Dual-zone heating architecture applies controlled thermal energy from both top and bottom heating plates, achieving ≤±0.5°C temperature uniformity across all wells under typical operating conditions (per ISO 20387 Annex B guidelines for laboratory equipment validation).
• Microprocessor-based PID temperature regulation delivers high stability (≤±0.2°C) and accuracy (<±0.3°C at 37°C), validated per ASTM E2573-21 for incubator performance assessment.
• Brushless DC motor ensures consistent rotational output over extended duty cycles (>10,000 hours MTBF), eliminating carbon brush wear and reducing mechanical drift in speed control (±1% deviation across full 300–1200 rpm range).
• Programmable timer supports durations from 1 minute to 99 hours 59 minutes; automatic audible alert signals completion, supporting unattended overnight protocols compliant with GLP documentation workflows.
• Power-loss recovery function retains active settings and resumes operation from the point of interruption—essential for long-duration kinetic assays where continuity affects data integrity.

Sample Compatibility & Compliance

The HN70-2A accommodates two standard microplates simultaneously—compatible with ANSI/SLAS-formatted 96-well, 384-well, and deep-well plates (including PCR and cell culture variants). Its compact footprint (284 × 264 × 157 mm) allows integration into multi-instrument workstations without compromising accessibility. The unit meets IEC 61010-1:2010 safety requirements for laboratory electrical equipment and complies with electromagnetic compatibility standards EN 61326-1:2013. While not certified for use in regulated GMP manufacturing environments, its temperature traceability, programmable audit log capability (via optional RS232/USB data export), and stable thermal profile support qualification under FDA 21 CFR Part 11–aligned internal SOPs when paired with validated calibration procedures.

Software & Data Management

Standalone operation requires no external software; however, optional serial communication (RS232) enables integration with laboratory information management systems (LIMS) or custom scripting environments (e.g., Python-based automation via PySerial). Time-stamped temperature and speed logs can be exported for inclusion in electronic lab notebooks (ELN) or regulatory submissions. Firmware supports user-defined protocol storage (up to 10 presets), each retaining independent setpoints for temperature, speed, amplitude, and duration—facilitating rapid reconfiguration between assay types without manual recalibration.

Applications

• ELISA plate washing and incubation steps requiring consistent agitation at 37°C or ambient conditions.
• Cell suspension maintenance during transfection or drug screening assays where shear-sensitive lines demand gentle yet effective mixing.
• Kinetic studies involving protein-protein interaction, ligand binding, or reporter gene expression where thermal homogeneity directly influences signal-to-noise ratio.
• Microbial growth monitoring in antibiotic susceptibility testing using broth microdilution methods (CLSI M07-A11 compliant setups).
• Pre-analytical sample preparation for downstream applications including qPCR, immunoassay, and flow cytometry—ensuring uniform reagent dispersion prior to analysis.

FAQ

Does the HN70-2A support external temperature probes for independent verification?

Yes—users may insert calibrated PT100 or NIST-traceable thermistors through designated access ports to cross-validate chamber temperature against the built-in sensor.
Can it accommodate non-standard plate formats, such as 24-well or custom microfluidic carriers?

The platform is mechanically optimized for ANSI/SLAS-compliant plates; alternative formats require third-party adapter frames and are not covered under warranty or performance specifications.
Is firmware update capability available?

Firmware updates are delivered via USB flash drive upon request from Hanuo technical support; version history and release notes are provided with each update package.
What is the recommended calibration interval for routine QC use?

Annual calibration is advised per ISO/IEC 17025:2017 guidance; users performing GLP-regulated work should establish internal intervals based on risk assessment and historical performance data.
Does the unit include validation documentation (IQ/OQ/PQ templates)?

Standard shipment includes basic OQ test scripts and acceptance criteria; full IQ/OQ/PQ documentation packages are available as optional add-ons for regulated laboratories.