Hanuo HN-08 Stomacher-Type Aseptic Homogenizer

Overview

The Hanuo HN-08 Stomacher-Type Aseptic Homogenizer is a precision-engineered laboratory instrument designed for gentle, reproducible mechanical homogenization of biological and food samples under controlled aseptic conditions. Based on the stomacher principle—where sample suspension is subjected to rhythmic, bidirectional compression between two parallel plates—the HN-08 delivers consistent shear forces without generating significant thermal load or cellular disruption beyond intended lysis. This makes it especially suitable for microbiological applications where viability preservation, colony-forming unit (CFU) recovery accuracy, and avoidance of cross-contamination are critical. The device operates within a fully enclosed stainless-steel chamber, isolating the sample from internal hardware via single-use sterile filter bags (17 × 30 cm), eliminating the need for post-run sterilization or cleaning of internal components. Its compact footprint (380 × 220 × 330 mm) and 22 kg mass ensure stable operation on standard laboratory benches while supporting routine high-throughput workflows in QC, clinical microbiology, and regulatory-compliant testing environments.

Key Features

• Stainless-steel working chamber with full-opening door and transparent polycarbonate observation window for real-time process monitoring.
• Large backlit LCD display with intuitive navigation interface supporting multilingual menu options (English default).
• Three independent programmable protocols—each storing custom combinations of time (1 s to 100 min, or continuous), beating frequency (3–12 cycles/sec), and beater gap (0–40 mm).
• Adjustable beater gap mechanism enables optimization for viscous, fibrous, or particulate-laden samples—critical for tissue homogenization and heterogeneous food matrices.
• No-contact sample processing: All material remains confined within certified sterile, low-binding polyethylene filter bags—eliminating carryover risk and reducing validation burden.
• Thermally neutral operation: No measurable temperature rise observed during standard runs (≤5 min at 8 cycles/sec), preserving microbial integrity and enzymatic activity.
• Minimal maintenance design: No internal seals, gaskets, or lubricated moving parts exposed to sample pathways—reducing downtime and calibration frequency.

Sample Compatibility & Compliance

The HN-08 accommodates a broad spectrum of sample types—including raw meat, poultry, seafood, leafy vegetables, dairy products, cosmetic emulsions, pharmaceutical suspensions, and environmental swab eluates—within its 3–400 mL volume range. Its stomacher-based action ensures efficient release of microorganisms from biofilm-embedded surfaces and intracellular compartments without excessive cell wall rupture. The system complies with core requirements of ISO 6887-1:2017 (microbiological examination of food and animal feeding stuffs), ASTM D5600-20 (standard guide for homogenization of environmental samples), and USP and (microbiological enumeration tests). When used with validated sterile bags meeting EN 14683:2019 filtration specifications, the platform supports GLP and GMP-aligned workflows, including audit-ready documentation of run parameters and operator identity via optional LIMS integration.

Software & Data Management

While the HN-08 operates as a standalone benchtop instrument, its embedded firmware logs all executed protocols—including timestamp, duration, frequency, gap setting, and user ID—into non-volatile memory (retention ≥10 years). These records can be exported via USB interface in CSV format for traceability and regulatory reporting. The system supports 21 CFR Part 11–compliant configurations when paired with third-party laboratory information management systems (LIMS) that enforce electronic signatures, audit trails, and role-based access control. No proprietary software installation is required; data files are natively readable in Excel, Python pandas, or statistical analysis platforms such as JMP or Minitab.

Applications

• Preparation of homogenates for aerobic plate counts, coliform detection, and pathogen enrichment per ISO 6579-1 and FDA BAM protocols.
• Standardized tissue dissociation for microbiome profiling, metagenomic DNA extraction, and culture-independent diagnostics.
• Homogenization of pharmacopeial dosage forms (e.g., creams, ointments, suspensions) prior to microbial limits testing (USP , EP 2.6.12).
• Environmental monitoring of cleanroom surfaces, water filters, and HVAC components using membrane filtration-compatible suspensions.
• QC release testing of ready-to-eat foods, infant formula, and dietary supplements under SQF, BRCGS, and FSSC 22000 schemes.

FAQ

What sterile bag specifications are compatible with the HN-08?
The instrument is optimized for 17 × 30 cm polyethylene filter bags with pore sizes ranging from 50 µm to 200 µm—certified for sterility (ISO 11137) and endotoxin-free handling.
Can the HN-08 be validated for ISO 17025-accredited laboratories?
Yes. Its programmable repeatability (±0.5% CV for time/frequency settings), mechanical stability, and parameter logging capability support IQ/OQ/PQ documentation per ISO/IEC 17025:2017 Annex A.3.
Is external cooling required during extended operation?
No. Thermal profiling confirms ambient temperature drift remains within ±0.8 °C at the bag surface over 15-minute continuous runs at maximum frequency—no active cooling is necessary.
How often does the beater assembly require recalibration?
The mechanical drive system is factory-calibrated and requires no field recalibration; periodic verification using certified reference weights and timing analyzers is recommended annually per ISO/IEC 17025.
Does the HN-08 support remote monitoring or Ethernet connectivity?
Not natively—but RS-232 and USB host interfaces enable integration with building automation systems or centralized lab monitoring platforms via middleware adapters.