DLAB TopPette Single-Channel Adjustable-Volume Manual Pipette
| Brand | DLAB |
|---|---|
| Origin | Beijing, China |
| Model | 20–200 µL |
| Volume Range | 20–200 µL |
| Maximum Capacity | 200 µL |
| Accuracy | ±2.5% |
| Precision (CV) | ±0.15% |
| Operating Principle | Air-displacement |
| Sterilization | Autoclavable (121 °C, 20 min) |
| Tip Compatibility | Standard conical universal pipette tips (300 µL) |
| Ergonomic Design | Full-hand grip, low actuation force, rotatable volume dial, lockable volume setting |
| Serviceability | Modular tip cone assembly, tool-free calibration access, replaceable filter barrier |
Overview
The DLAB TopPette Single-Channel Adjustable-Volume Manual Pipette is an engineered solution for precise, repeatable liquid handling in routine and regulated laboratory environments. Based on the air-displacement principle, it delivers consistent volumetric transfer across its calibrated range (20–200 µL) by generating a controlled vacuum within a sealed piston chamber. This mechanism ensures compatibility with aqueous, mildly viscous, and volatile liquids—provided appropriate tip selection and technique are applied. Designed for daily use in QC/QA labs, clinical diagnostics, academic research, and pharmaceutical development, the TopPette series adheres to ISO 8655-2:2022 requirements for manual piston-operated pipettes, supporting traceable performance verification and long-term metrological integrity.
Key Features
- Ergonomic full-hand grip with contoured geometry and balanced mass distribution—reduces operator hand fatigue during high-throughput workflows.
- Large, high-contrast digital volume window with tactile feedback dial; volume settings are mechanically locked to prevent accidental drift during operation or transport.
- Autoclavable lower assembly (tip cone and seal components) rated for repeated cycles at 121 °C for 20 minutes—ensuring compliance with GLP/GMP hygiene standards.
- Integrated hydrophobic filter barrier in the tip cone prevents aerosol contamination of the internal piston and cylinder, extending service life and safeguarding sample integrity.
- Modular tip cone design allows rapid disassembly using standard service tools—enabling field calibration, O-ring replacement, and piston cleaning without specialized equipment.
- Low-force plunger activation (≤1.8 N for aspiration, ≤2.2 N for dispensing) meets ISO 8655-5 ergonomic thresholds for sustained use.
- Universal conical tip interface compatible with all major 300 µL tip brands—validated per ISO 8655-3 for tip retention force and seal reliability.
Sample Compatibility & Compliance
The TopPette 20–200 µL pipette is validated for use with deionized water, buffered saline solutions (e.g., PBS), ethanol/water mixtures ( 10 mPa·s), corrosive (e.g., concentrated acids/bases), or dense organic solvents without prior validation. All units undergo factory calibration against NIST-traceable gravimetric standards per ISO 8655-6, with certificate of conformity supplied. The device supports audit-ready documentation: calibration logs, maintenance records, and user-defined SOPs can be maintained in accordance with FDA 21 CFR Part 11 when integrated into validated electronic lab notebook (ELN) or LIMS platforms.
Software & Data Management
As a fully manual instrument, the TopPette requires no embedded firmware or software. However, its metrological performance integrates seamlessly into digital quality systems via external calibration management software (e.g., MET/CAL, PipetteTrack, or LabVantage). Each unit carries a unique serial number engraved on the body for asset tracking. Calibration intervals, tolerance limits, and pass/fail criteria may be defined per ISO/IEC 17025 or internal QA protocols. Filter replacement and tip cone sterilization events should be recorded manually or via barcode-scanned log entries to satisfy GLP audit trails.
Applications
- Preparation of standard curves and dilution series in ELISA, qPCR, and spectrophotometric assays.
- Transfer of reagents and samples in clinical chemistry analyzers and hematology workstations.
- Cell seeding and media addition in 24-, 48-, and 96-well microplates.
- Sample aliquoting for biobanking, stability studies, and reference material preparation.
- Calibration of automated liquid handlers using gravimetric verification protocols.
FAQ
Is the TopPette 20–200 µL pipette autoclavable?
Yes—the lower tip cone assembly (including piston seal and filter housing) is fully autoclavable at 121 °C for 20 minutes. The upper body must be disassembled first; refer to DLAB Service Manual SM-TP-01 for approved sterilization sequence.
What calibration standard does DLAB follow?
All TopPette pipettes are calibrated gravimetrically per ISO 8655-6 using Class E1 stainless steel weights and certified analytical balances traceable to NIST. A factory calibration certificate is included with each unit.
Can this pipette be used with filtered tips?
Yes—standard 300 µL universal conical filtered tips (e.g., DLAB FT-300, Eppendorf epT.I.P.S., or Thermo Fisher Finntip) are fully compatible and recommended for applications requiring aerosol containment.
Does the volume lock function meet ISO 8655-2 requirements?
Yes—the mechanical volume locking mechanism prevents unintended adjustment during pipetting or storage, satisfying Clause 5.3.2 of ISO 8655-2:2022 for “protection against unintentional setting changes.”
How often should recalibration be performed?
DLAB recommends quarterly verification for routine use; more frequent checks (e.g., daily or per-shift) are required in GxP-regulated environments or after impact, disassembly, or exposure to extreme environmental conditions.
